Product related FAQ

Note: Please click product codes or arrows located right hand side of each FAQ box to open and close FAQ. Please click a product name for checking the product details.

Display the Number of Items

ELISA - IBL

  • Question
    Q.What is recommended dilution ratio?
  • Answer
    A.8 fold dilution is acceptable for the measurement, however, we recommend to dilute mouse urine sample more than 16 fold to avoid the data becomes range over.
  • Question
    Q.Which isoform of FGF21 can detect by this kit?
  • Answer
    A.This assay kit react to both full length FGF 21 (1-181) and FGF21 (7-181). Therefore, this kit can measure total FGF21.
  • Question
    Q.How much is the volume of sample required for measurement?
  • Answer
    A.5μL is recommended.
  • Question
    Q.How long does the plate coated biotinylated antibody become stable for use?
  • Answer
    A.We have confirmed that it is stable for a week.
  • Question
    Q.How long does streptavidin plate become stable for use after first opening the pack?
  • Answer
    A.We have confirmed that it is stable at 37℃ for 2 weeks.
  • Question
    Q.How much is the volume of sample required for measurement?
  • Answer
    A.5μL is recommended.
  • Question
    Q.How long does the plate coated biotinylated antibody become stable for use?
  • Answer
    A.We have confirmed that it is stable for a week.
  • Question
    Q.How long does streptavidin plate become stable for use after first opening the pack?
  • Answer
    A.We have confirmed that it is stable at 37℃ for 2 weeks.
  • Question
    Q.How much is the volume of sample required for measurement?
  • Answer
    A.2μL is acceptable for measuring GIP Total using samples collected after meal, however at least 5μL is required for measuring it using samples collected in fasting.
  • Question
    Q.How long does the plate coated biotinylated antibody become stable for use?
  • Answer
    A.We have confirmed that it is stable for a week.
  • Question
    Q.How long does streptavidin plate become stable for use after first opening the pack?
  • Answer
    A.We have confirmed that it is stable at 37℃ for 2 weeks.
  • Question
    Q.How much is the volume of sample required for measurement?
  • Answer
    A.2μL is acceptable for measuring GIP Total using samples collected after meal, however at least 5μL is required for measuring it using samples collected in fasting.
  • Question
    Q.How long does the plate coated biotinylated antibody become stable for use?
  • Answer
    A.We have confirmed that it is stable for a week.
  • Question
    Q.How long does streptavidin plate become stable for use after first opening the pack?
  • Answer
    A.We have confirmed that it is stable at 37℃ for 2 weeks.
99999
99999 ELISA - IBL common FAQ
  • Question
    Q.Is composition of EIA buffer of each ELISA kit all same? Can it be mixed to use?
  • Answer
    A.No it isn't. As constitute of each EIA buffer is different, it cannot be mixed with other lots or EIA buffers contained in other kind of ELISA kits.
  • Question
    Q.What is the composition of concentrated wash buffer?
  • Answer
    A.It contains ordinary Tween and phosphate buffer (0.05% Tween-20 in PB).
  • Question
    Q.What size of oligomer can be detected by this assay kit?
  • Answer
    A.Any oligomer that is bigger than Amyloidβ dimer and has a recognized region of 82E1 (N-terminal of 1-16) can be detected by this assay kit.
  • Question
    Q.Can this kit detect protofibril and fibril?
  • Answer
    A.It cross reacts with both protofibril and fibril.
  • Question
    Q.3 recognized parts of 82E1 antibody (a.a. 1-16) are existed if the sample is trimer. In that case, does the conjugated antibody binds to 2parts or only one part?
  • Answer
    A.82E1 antibody is used for as capture antibody (precoated one) and detection antibody (HRP conjugated one). Theoretically, 2 regions of trimer can bind to the detection antibody if 1 region is captured by captured antibody. However, this is only the case that actual molecule detected holds the N-terminal region of Aβ1-16.
  • Question
    Q.Is it depending on the size of oligomer to determine the quantity of the detection antibody that binds to one oligomer?
  • Answer
    A.It depends on molecule of oligomer. It does not detect monomer. We recommend to use both products #27718 Human Amyloidβ (1-40) (FL) Assay Kit - IBL and #27719 Human Amyloidβ (1-42) (FL) Assay Kit - IBL by depending on purposes.
  • Question
    Q.What is the condition of DPP 4 inhibitor?
  • Answer
    A.Only EDTA-plasma collected using collecting tubes contained DPP4 inhibitor can be used for measuring of GLP-1 active form. BD™ P800 GLP-1, GIP, Glucagon, Ghrelin storing vacuumed collecting tube should be used for collecting blood or the blood should be blocked its activity by DPP4 inhibitor prior it is measured. The amount of DPP4 inhibitor for use is 10μL per 1mL blood or 20μL per 1mL plasma.
  • Question
    Q.How can I store the standard after reconstitute it?
  • Answer
    A.It cannot be recommended to be stored after reconstitution as it is designed for use one-off. However, if you need to reuse it due to uncontrolable situation, please store it in frozen.
  • Question
    Q.Does this assay kit detect any specific type of VEGF or any type of VEGF such as A, B, C and D?
  • Answer
    A.It cross reacts with VEGF-A. Isoforms like 120, 164 and 188 can be measured as mixed substances if the sample is lysate.
  • Question
    Q.Is it possible to measure VEGF expressed in lysate of mice muscle?
  • Answer
    A.If it is an ordinary tissue lysate, it can be measured by the assay kit.
  • Question
    Q.What is the structure and host of the standard?
  • Answer
    A.It is originated from CHO supernatant and it is full length Flag-Tag.
  • Question
    Q.Can you verify that it is HIV or HBV negative?
  • Answer
    A.The plasma used as the material was verified as negative and not cross reactive against HbsAG, anti-HCV, anti-HBc by the method of approved by FDA.
  • Question
    Q.Can this kit be used for serum?
  • Answer
    A.No it cannot be used for normal serum.
  • Question
    Q.Is the extraction process required for serum samples?
  • Answer
    A.Yes, it is. Please refer to “12. Endothelin extraction process” on page 2 of the datasheet for additional information.
  • Question
    Q.What is the recommended dilution ratio for plasma samples?
  • Answer
    A.We recommend 40 fold dilution.
  • Question
    Q.Can this kit be used for serum or plasma samples?
  • Answer
    A.We do not have any data related to those types of samples.
  • Question
    Q.What is the epitope of the HRP conjugated antibody (Human APP (R101A4) Mouse IgG Fab’ A.P.)?
  • Answer
    A.We have not confirmed the epitope.
  • Question
    Q.What is the epitope of the pre-coated antibody (anti-human Gal-3 (50A3) mouse IgG MoAb)?
  • Answer
    A.The epitope of 50A3 is the 64 amino acid residue of the N-terminal region of Galectin-3.
  • Question
    Q.Does this antibody (the anti-human Gal-3 (50A3)) only bind to the 64th a.a. residue of Galectin-3?
  • Answer
    A.The antibody reacts to a.a. parts 1 to 64, which means the antibody can detect any part of Galectin-3 found in this portion (1 to 64). Thus, the antibody can also detect the full length of Galectin-3.
  • Question
    Q.Can this kit detect enzymatic activity?
  • Answer
    A.No it cannot.
  • Question
    Q.What is the standard derived from?
  • Answer
    A.It is derived from the supernatant of the human nerve glioblastoma cells (U251).
  • Question
    Q.Can this kit be used for heparin-plasma samples?
  • Answer
    A.We do not recommend using the kit for heparin plasma samples.
  • Question
    Q.Can this kit be used for serum samples?
  • Answer
    A.No, it cannot.
  • Question
    Q.Can this kit be used for serum samples?
  • Answer
    A.Osteopontin is rapidly digested by Thrombin in serum samples. For this reason, we do not recommend using serum samples for the assay because the value would be much lower.
  • Question
    Q.There are two types of EDTA-plasma tubes (Na and K). Which one is best to use?
  • Answer
    A.Either tube can be used.
  • Question
    Q.What is the epitope of the pre-coated antibody (Anti-Human OPN (O-17) Rabbit IgG A.P.)?
  • Answer
    A.The epitope is IPVKQADSGSSEEKQ.
  • Question
    Q.Can this kit be used to measure crab-eating macaque samples?
  • Answer
    A.We do not have any data related to those types of samples.
  • Question
    Q.Can this kit be used for citrated-plasma samples?
  • Answer
    A.We do not recommend using the kit for citrated-plasma samples.
  • Question
    Q.Can this kit be used for mouse or rat samples?
  • Answer
    A.No, they cannot.