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ELISA - IBL

  • Question
    Q.Can this kit be used for mouse or rat samples?
  • Answer
    A.No, it cannot.
    A monoclonal antibody (82E1) which is used as the labeled antibody in this kit, specifically reacts to N-terminal of Human Aβ.
    Since the N-terminal sequence of Aβ is different between human and rodents (mouse or rat), it does not cross reacts to mouse and/or rat Aβ.
  • Question
    Q.Can this kit be used for bovine samples?
  • Answer
    A.No, it cannot.
  • Question
    Q.Can this kit be used for bovine samples?
  • Answer
    A.No, it cannot.
  • Question
    Q.Does this kit detect Insulin I or Insulin II?
  • Answer
    A.This kit can be used for quantitatively measuring total insulin including Insulin I, Insulin II and Proinsulin.
  • Question
    Q.Does Neuramidase treatment require for the sample?
  • Answer
    A.No it doesn't. There is no much different for the result between with and without Neuramidase.
  • Question
    Q.What is epitope of the antibodies used in this kit?
  • Answer
    A.The HRP conjugated labeled antibody (Anti-Human APP (R101A4) Mouse IgG Fab’ Affinity Purify) used in this kit detects domain at N-Terminal.
    The epitope of the precoated plate antibody (Anti-Human sAPPα (2B3) Mouse IgG MoAb Affinity Purify at C-Terminal) used in this kit is DAEFRHDSGYEVHHQK.
  • Question
    Q.Does this kit cross reacts to mouse sAPPα if TG mice that express human sAPPα is used for the assay?
  • Answer
    A.No, it does not cross react to mouse sAPPα. This kit only detects human sAPPα.
  • Question
    Q.Does this kit cross react to BACE2?
  • Answer
    A.Not, it doesn't.
  • Question
    Q.What is recommended dilution ratio for human serum samples?
  • Answer
    A.The recommended dilution ratio for human serum sample is 2 fold.
  • Question
    Q.What type of soluble APP can be measured by this kit?
  • Answer
    A.This kit cross reacts to any type of human soluble APP.
  • Question
    Q.Does it cross react to mouse or rat Aβ (1-40)?
  • Answer
    A.No it doesn't.
  • Question
    Q.Does it cross react to mouse or rat Aβ (1-42)?
  • Answer
    A.No it doesn't.
  • Question
    Q.Can mouse or rat samples be used for this kit?
  • Answer
    A.Wild type mouse or rat samples cannot be used, but APP-transgenic (Tg2576) mouse can be used for the kit.
  • Question
    Q.Is there any recommended buffer solution for cell extraction?
  • Answer
    A.We recommend to use our product #19022 IBLysis.
  • Question
    Q.What is the epitope of the antibodies used in this kit?
  • Answer
    A.The epitope of HRP conjugated labeled antibody (Anti-Human Aβ (N) (82E1) Mouse IgG MoAb Affinity Purify at N-Terminal) used in this kit is DAEFRHDSGYEVHHQK.
    The epitope of the precoated plate antibody (Anti-Human APP-C Rabbit IgG Affinity Purify at C terminal) used in this kit is NGYENPTYKFFEQMQN.
  • Question
    Q.Can serum samples be used for this kit?
  • Answer
    A.No, it cannot.
  • Question
    Q.Can serum samples be used for this kit?
  • Answer
    A.Not recommended to use serum samples. Please use EDTA-plasma samples.
  • Question
    Q.What is recommended anticoagulant?
  • Answer
    A.We use 2Na.
  • Question
    Q.Can VEGF-R2 be measured by this kit?
  • Answer
    A.No, it cannot.
  • Question
    Q.What is recommended dilution ratio for serum samples?
  • Answer
    A.It is 2 to 8 fold.
  • Question
    Q.What is the condition of DPP 4 inhibitor?
  • Answer
    A.Only EDTA-plasma collected using collecting tubes contained DPP4 inhibitor can be used for measuring of GLP-1 active form. BD™ P800 GLP-1, GIP, Glucagon, Ghrelin storing vacuumed collecting tube should be used for collecting blood or the blood should be blocked its activity by DPP4 inhibitor prior it is measured. The amount of DPP4 inhibitor for use is 10μL per 1mL blood or 20μL per 1mL plasma.
  • Question
    Q.What kind of degradative enzyme inhibitor is suitable for use?
  • Answer
    A.We use DPP4 inhibitor.
  • Question
    Q.What is recommended dilution ratio for blood samples?
  • Answer
    A.Please start to dilute from 2 fold.
  • Question
    Q.Can serum samples be used for this kit?
  • Answer
    A.Not recommended to use serum samples.
  • Question
    Q.Can serum sample be used for this kit?
  • Answer
    A.Not recommended to use serum samples. Please refer to the following article for comparison data between serum and plasma. https://www.ncbi.nlm.nih.gov/pubmed/?term=25045139
  • Question
    Q.Can frozen serum sample be used for this kit?
  • Answer
    A.Yes, it can. However, stability test of the samples should be conducted and confirmed by each institute.
  • Question
    Q.What is recommended dilution ratio?
  • Answer
    A.8 fold dilution is acceptable for the measurement, however, we recommend to dilute mouse urine sample more than 16 fold to avoid the data becomes range over.
  • Question
    Q.Can rat sample be used for this kit?
  • Answer
    A.No, it cannot.
  • Question
    Q.Is stability data available?
  • Answer
    A.It is available on the following relevant product page as images.
    http://www.ibl-japan.co.jp/en/search/product/detail/id=4678
  • Question
    Q.Which isoform of FGF21 can detect by this kit?
  • Answer
    A.This assay kit react to both full length FGF 21 (1-181) and FGF21 (7-181). Therefore, this kit can measure total FGF21.
  • Question
    Q.How much is the volume of sample required for measurement?
  • Answer
    A.5μL is recommended.
  • Question
    Q.How long does the plate coated biotinylated antibody become stable for use?
  • Answer
    A.We have confirmed that it is stable for a week.
  • Question
    Q.How long does streptavidin plate become stable for use after first opening the pack?
  • Answer
    A.We have confirmed that it is stable at 37℃ for 2 weeks.
  • Question
    Q.How much is the volume of sample required for measurement?
  • Answer
    A.5μL is recommended.
  • Question
    Q.How long does the plate coated biotinylated antibody become stable for use?
  • Answer
    A.We have confirmed that it is stable for a week.
  • Question
    Q.How long does streptavidin plate become stable for use after first opening the pack?
  • Answer
    A.We have confirmed that it is stable at 37℃ for 2 weeks.
  • Question
    Q.How much is the volume of sample required for measurement?
  • Answer
    A.2μL is acceptable for measuring GIP Total using samples collected after meal, however at least 5μL is required for measuring it using samples collected in fasting.
  • Question
    Q.How long does the plate coated biotinylated antibody become stable for use?
  • Answer
    A.We have confirmed that it is stable for a week.
  • Question
    Q.How long does streptavidin plate become stable for use after first opening the pack?
  • Answer
    A.We have confirmed that it is stable at 37℃ for 2 weeks.