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#18771 Anti- Cre recombinase Rabbit IgG Affinity Purify

  • WB
  • TG-Brain
  • TG-Kidney
  • TG-Pancreas
Intended Use：
Research reagents
Application：
WB, IHC
Package Size1：
50 μg
Package Size2：
5 μg
Note on Application Abbreviations
WB:Western Blotting
IHC:Immunohistochemistry

※ The product indicated as "Research reagents" in the column Intended Use cannot be used
　 for diagnostic nor any medical purpose.
※ The datasheet listed on this page is sample only. Please refer to the datasheet
　 enclosed in the product purchased before use.

Product Overview

Product Overview

Product Code 18771
Product Name Anti- Cre recombinase Rabbit IgG Affinity Purify
Intended Use Research reagents
Application WB, IHC
Immunizing antigen Synthetic peptide of the middle part　of Cre　recombinase(MLHRRSGLPRPSDSNAV)
Purification Method Affinity Purified with antigen peptide
Specificity Confirmed by western blotting using Cre Cos/TNE sup.
Package Form Lyophilized product from PBS containing 1 % BSA and 0.05 % NaN3
Storage Condition 2 - 8℃
Poisonous and Deleterious Substances Applicable
Cartagena Not Applicable
Package Size 1 50 μg
Package Size 2 5 μg
Remarks1 The commercial use of products without our permission is prohibited. Please make sure to contact us and obtain permission.

Product Description

Product Description

Cre is a 38 kDa recombinase protein from bacteriophage P1 which mediates intramolecular and intermolecular site specific recombination between loxP sites. The role of Cre is to resolve dimers of P1 that arise after replication in order to allow partitioning of the two P1 molecules at cell division. A loxP site (locus of X-ing over) consists of two 13 bp inverted repeats separated by an 8 bp asymmetric spacer region. One molecule of Cre binds per inverted repeat or two Cre molecules line up at one loxP site. The recombination occurs in the asymmetric spacer region. Those 8 bases are also responsible for the directionality of the site. Two loxP sequences in opposite orientation to each other invert the intervening piece of DNA, two sites in direct orientation dictate excision of the intervening DNA between the sites leaving one loxP site behind. Thus, this precise removal of DNA can be used to eliminate an endogenous gene or transgene activate a transgene.
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