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#27181 Human ApoB-100 Assay Kit - IBL

  • #27181 Human ApoB-100 ELISA Kit
  • #27181 Human ApoB-100 ELISA Kit
  • #27181 Human ApoB-100 ELISA Kit
  • #27181 Human ApoB-100 ELISA Kit
  • #27181 Human ApoB-100 ELISA Kit
  • #27181 Human ApoB-100 ELISA Kit
  • #27181 Human ApoB-100 ELISA Kit
Intended Use：
Research reagents
Measuring Method：
ELISA
Sample Types：
Human
Measuring Samples：
Serum, EDTA-plasma, Cell culture supernatant
Measurement Range：
0.13 - 8.4 μg/mL
Package Size1：
96 Well

※ The product indicated as "Research reagents" in the column Intended Use cannot be used
　 for diagnostic nor any medical purpose.
※ The datasheet listed on this page is sample only. Please refer to the datasheet
　 enclosed in the product purchased before use.

Product Overview

Product Overview

Product Code 27181
Product Name Human ApoB-100 Assay Kit - IBL
Intended Use Research reagents
Measuring Method ELISA
Conjugate HRP
Species Human
Measuring Samples Serum, EDTA-plasma, Cell culture supernatant
Measurement Range 0.13 - 8.4 μg/mL
Primary Reaction 60 minutes at 37 ℃
Secondary Reaction 30 minutes at 2 - 8 ℃
Sensitivity 0.03 μg/mL
Specificity Substance Cross Reactivity
Human ApoB-100 100.0%
Human ApoB-48 < 0.1 %
Storage Condition 2 - 8 ℃
Poisonous and Deleterious Substances Not Applicable
Cartagena Not Applicable
Measuring Service Available
Package Size 1 96 Well

Product Description

Product Description

Though the lipids (such as fat and cholesterol) are not soluble in water, apolipoproteins are carrier proteins that combine with lipids to form lipoprotein particles which are water-soluble and can be carried through water-based circulation (i.e., blood, lymph). Apolipoproteins are classified according to their forms and functions, and there are six major classes and several sub-classes. ApoB (ApolipoproteinB) is the primary apolipoprotein of LDL and is considered to reflect change of LDL level in blood. There are two types apolipoprotein in ApoB. One is ApoB-100 which correlates with cholesterol level well and is released as LDL and VLDL. Another is ApoB-48 which presents being incorporated into chylomicrons and correlates well with tryglyceride. Generally, the concentration of ApoB in blood has been measured as the total of ApoB-100 and ApoB-48. This kit can measure only ApoB-100 in plasma samples using ApoB-100 specific antibodies which don’t recognize ApoB-48. This highly sensitive sandwich ELISA can also measure ApoB-100 in cell culture media or various fractionated lipoprotein samples.

FAQ

FAQ

  • Question
    Q.Can you verify that it is HIV or HBV negative?
  • Answer
    A.The plasma used as the material was verified as negative and not cross reactive against HbsAG, anti-HCV, anti-HBc by the method of approved by FDA.
  • Question
    Q.What is used for the standard?
  • Answer
    A.It is ApoB 100 that is purified from human plasma.
  • Question
    Q.Is composition of EIA buffer of each ELISA kit all same? Can it be mixed to use?
    ELISA common FAQ
  • Answer
    A.No it isn't. As constitute of each EIA buffer is different, it cannot be mixed with other lots or EIA buffers contained in other kind of ELISA kits.
  • Question
    Q.What is the composition of concentrated wash buffer?
    ELISA common FAQ
  • Answer
    A.It contains ordinary Tween and phosphate buffer (0.05% Tween-20 in PB).
  • Question
    Q.What is the feature of the plate?
    ELISA common FAQ
  • Answer
    A.We use plate that is flat bottom and removable strip type plate (8wellx 12 strips).
  • Question
    Q.Can I re-use standard after reconstitution?
    ELISA common FAQ
  • Answer
    A.Not recommended to re-use standard after reconstitution. Please use it at once after the reconstitution.
    Please note that there are some exceptions. One time freeze-thaw the standard is acceptable for use after reconstitution for some ELISAs.
    Please check the details on each product datasheet.
  • Question
    Q.What is different between reagent blank and test sample blank?
    ELISA common FAQ
  • Answer
    A.Reagent blank means a well is only added EIA buffer and the purpose is confirming whether the Test sample value is influenced by lack of washing process or other operations. Test sample blank means a well is added EIA buffer and HRP antibody and the purpose is to calculate the background.
  • Question
    Q.How many samples can be measured by this kit?
    ELISA common FAQ
  • Answer
    A.The pre-coated plate contained in our ELISA kit is 96 wells plate. We recommend to use 16 wells (2 slits) for standard and 80 wells (10 slits) for 40 samples in duplicate.
  • Question
    Q.What is LOD (Limit of Detection)?
    ELISA common FAQ
  • Answer
    A.It (LOD) is defined as sensitivity that is calculated using the NCCSL method. Please refer to a datasheet of each product.
  • Question
    Q.What is LOQ (Limit of Quantification)?
    ELISA common FAQ
  • Answer
    A.It (LOQ) is the lowest value of measurement (standard) range. Please refer to a datasheet of each product.
  • Question
    Q.What is the definition of Over Night (O/N) reaction?
    ELISA common FAQ
  • Answer
    A.It means that the reaction is required more than 16 hours unless otherwise specifically defined it on a datasheet of each ELISA product.
  • Question
    Q.What is the specification of quality control for ELISA product release?
    ELISA common FAQ
  • Answer
    A.The information of specification is available on individual lot specific CoA. Please contact us with your reference lot number for obtaining of specific CoA.
  • Question
    Q.What is the number (e.g. 432143214321) at the edge of strips of the plate?
    ELISA common FAQ
  • Answer
    A.According to the plate maker (ThermoFisher), it does not have any specific meaning as it is just the number of molds.
  • Question
    Q.How to wash an ELISA plate?
    ELISA common FAQ
  • Answer
    A.Washing it by an auto-washer is highly recommended.
    If it is not available, please refer to the demo video (only 2 mins) using a washing bottle.
  • Question
    Q.The wells turned black during the test with the kit.
    ELISA common FAQ
  • Answer
    A.It is possible that the wells were not washed sufficiently during the washing process after the HRP-labeled antibody reaction.
    Be sure to wash the wells enough times as described in the data sheet with washing buffer of more than 350 µL.
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