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#27258 Human Osteopontin N-Half Assay Kit - IBL

Intended Use:
Research reagents
Measuring Method:
ELISA
Sample Types:
Human
Measuring Samples:
EDTA-plasma, Urine, Synovial fluid, Cell culture supernatant
Measurement Range:
6.25 - 400 pmol/L
Package Size1:
96 Well

※ The product indicated as "Research reagents" in the column Intended Use cannot be used
for diagnostic nor any medical purpose.

Product Overview

Product Code 27258
Product Name Human Osteopontin N-Half Assay Kit - IBL
Intended Use Research reagents
Measuring Method ELISA
Conjugate HRP
Species Human
Measuring Samples EDTA-plasma, Urine, Synovial fluid, Cell culture supernatant
Measurement Range 6.25 - 400 pmol/L
Primary Reaction 60 minutes at 37 ℃
Secondary Reaction 30 minutes at 2 - 8 ℃
Sensitivity 3.09 pmol/L
Storage Condition 2 - 8 ℃
Poisonous and Deleterious Substances Not Applicable
Cartagena Not Applicable
Measuring Service Not Available
Package Size 1 96 Well

Product Description

Osteopontin (OPN) is a secretory glycoprotein first isolated from the bone. At present, it is known as a highly acidic calcium-binding phosphorylated protein with sugar chain bonds, that is secreted from diverse cells, including osteoblasts, renal tubular cells, macrophages, activated T lymphocytes and vascular smooth muscle cells. Its molecular weight can vary depending on the sugar chain formation and phosphorylation, and has been reported to range from 44 to 66 kDa. One major characteristic of OPN is that its molecule contains an Arg-Gly-Asp (RGD) amino acid sequence. This motif is also seen in fibronectin, vitronectin and other extracellular proteins. It is known that OPN binds through this motif to members of the integrin family (e.g., αvβ3) of cell surface receptors. Another unique characteristic of OPN is that it can assume various molecular forms in vivo by undergoing RNA splicing, saccharification, phosphorylation, sulfation, degradation by protease, etc. OPN is considered to coexist with thrombin locally in tissues such as injured tissue, inflamed tissue, vascularized tissue, and tumor tissue. Coexistence with thrombin increases the likelihood of proteolysis, and this process may have an important physiological role. The cleavage of OPN with thrombin causes the hidden sequence of the adhering sites to be exposed. It has been reported that the fragments of OPN produced following cleavage by thrombin react with α9β1 integrin. Furthermore, in view of the finding that OPN has many cell-binding sites and can react with various receptors, interactions between OPN and these cells or receptors may play some unknown roles. This kit can specifically measure the N-terminal OPN fragment (hereinafter called “OPN N-Half”) cleaved by thrombin. OPN molecules without thrombin cleavage, on the other hand, are hardly detected by the kit.

FAQ

  • Question
    Q.Can this kit be used for crab-eating macaque samples?
  • Answer
    A.We do not have any data related to those types of samples.
  • Question
    Q.Can this kit be used for rat samples?
  • Answer
    A.No, it cannot.
  • Question
    Q.Can this kit be used for bovine samples?
  • Answer
    A.No, it cannot.
  • Question
    Q.Is composition of EIA buffer of each ELISA kit all same? Can it be mixed to use?
    ELISA common FAQ
  • Answer
    A.No it isn't. As constitute of each EIA buffer is different, it cannot be mixed with other lots or EIA buffers contained in other kind of ELISA kits.
  • Question
    Q.What is the composition of concentrated wash buffer?
    ELISA common FAQ
  • Answer
    A.It contains ordinary Tween and phosphate buffer (0.05% Tween-20 in PB).
  • Question
    Q.What is the feature of the plate?
    ELISA common FAQ
  • Answer
    A.We use plate that is flat bottom and removable strip type plate (8wellx 12 strips).
  • Question
    Q.Can I re-use standard after reconstitution?
    ELISA common FAQ
  • Answer
    A.Not recommended to re-use standard after reconstitution. Please use it at once after the reconstitution.
    Please note that there are some exceptions. One time freeze-thaw the standard is acceptable for use after reconstitution for some ELISAs.
    Please check the details on each product datasheet.
  • Question
    Q.What is different between reagent blank and test sample blank?
    ELISA common FAQ
  • Answer
    A.Reagent blank means a well is only added EIA buffer and the purpose is confirming whether the measuring value is influenced by lack of washing process. Test sample blank means a well is added EIA buffer and HRP antibody and the purpose is to calculate the background.
  • Question
    Q.How many samples can be measured by this kit?
    ELISA common FAQ
  • Answer
    A.The pre-coated plate contained in our ELISA kit is 96 wells plate. We recommend to use 16 wells (2 slits) for standard and 80 wells (10 slits) for 40 samples in duplicate.
  • Question
    Q.What is LOD (Limit of Detection)?
    ELISA common FAQ
  • Answer
    A.It (LOD) is defined as sensitivity that is calculated using the NCCSL method. Please refer to a datasheet of each product.
  • Question
    Q.What is LOQ (Limit of Quantification)?
    ELISA common FAQ
  • Answer
    A.It (LOQ) is the lowest value of measurement (standard) range. Please refer to a datasheet of each product.

Note: This product is for research use only. It cannot be used as a diagnostic tool or drug.