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#27416 Mouse sAPPβ-w Assay Kit - IBL

  • #27416 Mouse sAPPβ-w ELISA Kit
  • #27416 Mouse sAPPβ-w ELISA Kit
  • #27416 Mouse sAPPβ-w ELISA Kit
  • #27416 Mouse sAPPβ-w ELISA Kit
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Intended Use:
Research reagents
Measuring Method:
ELISA
Sample Types:
Mouse
Measuring Samples:
Plasma, Cell culture supernatant
Measurement Range:
13 ~ 800 pg/mL
Package Size1:
96 Well

Product Overview

Product Code 27416
Product Name Mouse sAPPβ-w Assay Kit - IBL
Maker Name Immuno-Biological Laboratories Co., Ltd.
Intended Use Research reagents
Measuring Method ELISA
Conjugate HRP
Species Mouse
Measuring Samples Plasma, Cell culture supernatant
Measurement Range 13 ~ 800 pg/mL
Primary Reaction 2~8°C Overnight
Secondary Reaction 2~8°C 60minutes
Sensitivity 2.87 pg/mL
Specificity Mouse sAPPα <1%
Storage Condition 2 ~ 8 ℃
Poisonous and Deleterious Substances Not Applicable
Cartagena Not Applicable
Measuring Service Not Available
Package Size 1 96 Well

Product Description

Alzheimer's disease (AD) was first reported by A. Alzheimer, a German neuropathologist in 1907 and is considered as a major factor of dementia. Regarding the cause of Alzheimer’s disease, the Amyloid cascade hypothesis that suggests accumulating of peptide so-called Amyloidβ (Aβ) in the brain become a trigger and it causes death of neuron is widely supported. It is known that Aβ is produced by cleaving by 2 types of secretase called β secretase and γ secretase. Firstly it is cleaved by β secretase and subsequently cleaved by γ secretase from Amyloid Precursor Protein “APP” that is membrane protein consists of 695, 751 or 770 amino acids.Since soluble APPβ (sAPPβ) produced by β-secretase cleavage corresponds to Aβ production accordingly, it is desired to measure sAPPβ in parallel with Aβ. On the one hand, it is considered that the metabolic pathway of APP is normally started by cleaving by α-secretase rather than β-secretase and soluble APPα (sAPPα) is produced and subsequently molecule called “P3” is cleaved by γ-secretase.Current drug research and development of Alzheimer’s disease has been progressing based on the Amyloid cascade hypothesis and especially Aβ production suppressed by regulation of secretase activity is focused. Measuring sAPPα and sAPPβ in parallel with Aβ for research and evaluation of secretase inhibitor is valuable.This kit can only measure soluble sAPPβ in these 2 types of soluble APP in mouse samples.

FAQ

  • Question
    Q.Is composition of EIA buffer of each ELISA kit all same? Can it be mixed to use?
    ELISA common FAQ
  • Answer
    A.No it isn't. As constitute of each EIA buffer is different, it cannot be mixed with other lots or EIA buffers contained in other kind of ELISA kits.
  • Question
    Q.What is the composition of concentrated wash buffer?
    ELISA common FAQ
  • Answer
    A.It contains ordinary Tween and phosphate buffer (0.05% Tween-20 in PB).
  • Question
    Q.What is the feature of the plate?
    ELISA common FAQ
  • Answer
    A.We use plate that is flat bottom and removable strip type plate (8wellx 12 strips).
  • Question
    Q.Can I re-use standard after reconstitution?
    ELISA common FAQ
  • Answer
    A.Not recommended to re-use standard after reconstitution. Please use it at once after the reconstitution.
    Please note that there are some exceptions. One time freeze-thaw the standard is acceptable for use after reconstitution for some ELISAs.
    Please check the details on each product datasheet.
  • Question
    Q.What is different between reagent blank and test sample blank?
    ELISA common FAQ
  • Answer
    A.Reagent blank means a well is only added EIA buffer and the purpose is confirming whether the measuring value is influenced by lack of washing process. Test sample blank means a well is added EIA buffer and HRP antibody and the purpose is to calculate the background.
  • Question
    Q.How many samples can be measured by this kit?
    ELISA common FAQ
  • Answer
    A.The pre-coated plate contained in our ELISA kit is 96 wells plate. We recommend to use 16 wells (2 slits) for standard and 80 wells (10 slits) for 40 samples in duplicate.
  • Question
    Q.What is LOD (Limit of Detection)?
    ELISA common FAQ
  • Answer
    A.It (LOD) is defined as sensitivity that is calculated using the NCCSL method. Please refer to a datasheet of each product.
  • Question
    Q.What is LOQ (Limit of Quantification)?
    ELISA common FAQ
  • Answer
    A.It (LOQ) is the lowest value of measurement (standard) range. Please refer to a datasheet of each product.
  • Question
    Q.Do you have any protocol for preparation of mice and/or rat brain
    tissue extracts?
    (ELISA common FAQ * Specified FAQ for ELISA that is applicable for brain tissue extract.)
  • Answer
    A.CHAPS is recommended for extractin of wild type (W.T.) mice or rat brain tissue.
    Please refer to the following link for the brain tissue extraction process using CHAPS.
    http://www.ibl-japan.co.jp/files/topics/4944_ext_02_0.pdf

    Guanidine solution is recommended for extraction of Transgenic (Tg) mice or rat brain tissue.
    Please refer to the following link and the following two scientific articles for brain tissue extraction process using Guanidine.
    http://www.ibl-japan.co.jp/files/topics/4945_ext_02_0.pdf

    Presenilin-2 mutation causes early amyloid accumulation and memory impairment in a transgenic mouse model of Alzheimer's disease. Toda T et al. J Biomed Biotechnol. 2011;2011:617974.
    https://www.ncbi.nlm.nih.gov/pubmed/?term=21234330

    Methylene blue modulates β-secretase, reverses cerebral amyloidosis, and improves cognition in transgenic mice. Mori T et al. J Biol Chem. 2014 Oct 31;289(44):30303-17.
    https://www.ncbi.nlm.nih.gov/pubmed/?term=25157105

    * The ELISA kit #27712 Aβ42 and #27714 Aβ40 have been upgraded to #27719 Aβ (1-42)(FL) and #27718 Aβ (1-40)(FL).

Note: This product is for research use only. It cannot be used as a diagnostic tool or drug.