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#10777 Anti-Human Gd-IgA1(KM55) Rat IgG MoAb

  • IHC using KM55
Intended Use:
Research reagents
Application:
IHC
Package Size1:
100 μg
Package Size2:
10 μg
Note on Application Abbreviations
IHC:Immunohistochemistry

※ The product indicated as "Research reagents" in the column Intended Use cannot be used
  for diagnostic nor any medical purpose.
※ The datasheet listed on this page is sample only. Please refer to the datasheet
  enclosed in the product purchased before use.

Product Overview

Product Overview

Product Code 10777
Product Name Anti-Human Gd-IgA1(KM55) Rat IgG MoAb
Maker Name Immuno-Biological Laboratories Co., Ltd.
Intended Use Research reagents
Application IHC
Species Human
Immunizing antigen Human IgA1 hinge region peptide with GalNAc H-C223PST*PPT*PS*PS*TPPT*PSPS240-NH2(*with GalNAc)
Source Mouse-Rat hybridoma
Clone Name KM55
Subclass Rat IgG2b
Purification Method Affinity purified with protein A
Package Form Lyophilized product from PBS containing 1 % BSA and 0.05 % NaN3
Storage Condition 2 ~ 8 ℃
Poisonous and Deleterious Substances Applicable
Cartagena Not Applicable
Package Size 1 100 μg
Package Size 2 10 μg
Remarks1 The commercial use of products without our permission is prohibited. Please make sure to contact us and obtain permission.

Product Description

Product Description

Galactose-deficient IgA1 (Gd-IgA1) attracts a lot of attentions as a critical effector molecule in the pathogenesis and progression of IgA nephropathy (IgAN) in recent studies. It has been suggested that several O-link glycans modified regions exist in the heavy chain hinge region of human IgA1 molecule andGd-IgA1 circulates in blood stream of the patients with the pathological condition of IgAN. The deposition of Gd-IgA1 in glomeruli is considered to be involved in IgAN (Multi-hit Hypothesis of Gd-IgA1). The measuring system using snail (Helix aspersa; HAA) lectin that is extracted from snail and Gd-IgA1 ELISA (Code: 27600 Gd-IgA1 (Galactose-deficient IgA1) Assay Kit using this antibody (KM55) have been used in numerous studies and it has been suggested that serum level of Gd-IgA1 in patients with IgAN is signicficantly elevated compare with the level of healthy subject or patients with renal disease other than IgAN in such studies. In addition, this antibody (KM55) can detect Gd-IgA1 in tissue by IHC technique differently from the feature of HAA lectin and it has been revealed that Gd-IgA1 specicillay exists in glomeruli of the patients of IgAN from the studies using this antibody. It has been expected that clinical significance of Gd-IgA1 wil be revealed by further studies using this antibody.

References

References

Note: Retrieve by PMID number in displayed by abstract: http://www.ncbi.nlm.nih.gov

FAQ

FAQ

  • Question
    Q.What is the recommended concentration of this antibody for use?
  • Answer
    A.It is 100μg/mL.
  • Question
    Q.I am struggling for staining using this antibody. Can you recommend any method for IHC?
  • Answer
    A.Please refer to the method written in this article.
  • Question
    Q.How much is approx. normal IgA in blood?
  • Answer
    A.Total IgA is approx 1-4mg/mL in blood.
  • Question
    Q.How much is approx. normal Gd-IgA in blood?
  • Answer
    A.As it is approx 1,000 ng/mL in blood, more than 200 fold dilution is recommended for measurement.
  • Question
    Q.Where the antibody (Anti-Gd-IgA1(KM55) rat IgG) recognize?
  • Answer
    A.It recognizes PST(GalNAc)PP.
    Please refer to this article.
  • Question
    Q.According to the data sheet of the antibody (KM55), immunogen activation treatment is required when performing immunohistochemical staining (IHC) using KM55 with formalin fixed paraffin embedded tissues.
    Why is the treatment required?
  • Answer
    A.The targeted antigen might be masked by the fixation process in immunohistochemical staining.
    For avoiding such masking effects, immunogen activation (enzymatic treatment or heat treatment) is required for exposing the targeted antigen.
    Subtilisin A treatment is recommended for IHC using the antibody (KM55).
  • Question
    Q.I am considering to conduct immunohistochemistry. How many prepalates can be stained with 100ug?
  • Answer
    A.KM55 is 100ug/1mL.
    Usually, 0.1-0.2mL/preparate is used. This means 5-10 prepalates can be stained.

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