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#27261 Human DMP1 Assay Kit - IBL

  • Human DMP1
  • Human DMP1
  • Human DMP1
  • Human DMP1
  • Human DMP1
  • Human DMP1
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Intended Use:
Research reagents
Measuring Method:
ELISA
Sample Types:
Human
Measuring Samples:
Serum, EDTA-plasma, Urine, Cell culture supernatant
Measurement Range:
3.6 ~ 230 pmol/L
Package Size1:
96 Well

Product Overview

Product Code 27261
Product Name Human DMP1 Assay Kit - IBL
Maker Name Immuno-Biological Laboratories Co., Ltd.
Intended Use Research reagents
Measuring Method ELISA
Conjugate HRP
Species Human
Measuring Samples Serum, EDTA-plasma, Urine, Cell culture supernatant
Measurement Range 3.6 ~ 230 pmol/L
Primary Reaction Overnight at 2 - 8 ℃
Secondary Reaction 60 minutes at 2 - 8 ℃
Sensitivity 0.57 pmol/L
Specificity Substance Cross Reactivity
Mouse DMP-1 ≦0.1%
Rat DMP-1 ≦0.1%
Storage Condition 2 - 8 ℃
Poisonous and Deleterious Substances Not Applicable
Cartagena Not Applicable
Measuring Service Available
Package Size 1 96 Well

Product Description

Dentin Matrix Protein 1(DMP1) is a constitutive protein in extracellular matrix of osteocyte and it belongs to Small Integrin-Binding Ligand, N-linked Glycoprotein (SIBLING) family. It is synthesized as a full-length 105kDa proprotein and it is divided into 37kDa at N terminal side and 57kDa at C terminal side in the secretion process. It has a lot of acidic domains and it binds with calcium because of the feature that become negative charged within tissues and it has an important role for bone mineralization. DMP1 expresses in osteocyte while other constitutive proteins in extracellular matrix such as Osteopontin, Osteocalcin and Bone sialoprotein express in osteoblast cells. Osteocyte which is a main constituent cell of bone regulates activities of osteoclastic cells and osteoblast cells and it adjusts bone remodeling process. It also acts as an endocrine cell which secrets FGF-23. DMP1 in blood is considered as a candidate of biomarker which reflects activities of osteocyte and it is also suggested to become useful information for research of bone metabolism and phosphate metabolism. The concentration of DMP1 in human blood can be quantitatively measured by this ELISA kit.

FAQ

  • Question
    Q.Is composition of EIA buffer of each ELISA kit all same? Can it be mixed to use?
  • Answer
    A.No it isn't. As constitute of each EIA buffer is different, it cannot be mixed with other lots or EIA buffers contained in other kind of ELISA kits.
  • Question
    Q.What is the composition of concentrated wash buffer?
  • Answer
    A.It contains ordinary Tween and phosphate buffer (0.05% Tween-20 in PB).
  • Question
    Q.What is the feature of the plate?
  • Answer
    A.We use plate that is flat bottom and removable strip type plate (8wellx 12 strips).
  • Question
    Q.Can I re-use standard after reconstitution?
  • Answer
    A.Not recommended to re-use standard after reconstitution. Please use it at once after the reconstitution.
  • Question
    Q.What is different between reagent blank and test sample blank?
  • Answer
    A.Reagent blank means a well is only added EIA buffer and the purpose is confirming whether the measuring value is influenced by lack of washing process. Test sample blank means a well is added EIA buffer and HRP antibody and the purpose is to calculate the background.
  • Question
    Q.How many samples can be measured by this kit?
  • Answer
    A.The pre-coated plate contained in our ELISA kit is 96 wells plate. We recommend to use 16 wells (2 slits) for standard and 80 wells (10 slits) for 40 samples in duplicate.

Note: This product is for research use only. It cannot be used as a diagnostic tool or drug.