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#27264 LPL/HTGL Activity Control Plus Kit - IBL

  • LPL/HTGL Activity Control Plus Kit
  • LPL/HTGL Activity Control Plus Kit
  • LPL/HTGL Activity Control Plus Kit
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Intended Use:
Research reagents
Sample Types:
Human
Package Size1:
1 Kit

Product Overview

Product Code 27264
Product Name LPL/HTGL Activity Control Plus Kit - IBL
Maker Name Immuno-Biological Laboratories Co., Ltd.
Intended Use Research reagents
Species Human
Storage Condition 2 - 10 ℃
Poisonous and Deleterious Substances Applicable
Cartagena Not Applicable
Package Size 1 1 Kit
Remarks1 Control set is included in this kit.
Please refer to the following product if you don't need the control set.
#27185 LPL/HTGL Activity Assay Kit - IBL

Product Description

This kit can measure the activities of lipoprotein lipase (LPL) and hepatic triglyceride lipase (HTGL) in post-heparin plasma. LPL plays a central role in lipoprotein metabolism by catalyzing hydrolysis of triglycerides (TGs) in chylomicrons and VLDL particles. Patients with LPL deficiency present with marked hypertriglyceridemia with accumulation of chylomicrons.
HTGL is synthesized by hepatocytes and bound to heparin sulfate proteoglycans at the surface of liver sinusoidal capillaries which hydrolyzes TGs and phospholipids in chylomicron remnants, intermediate density lipoproteins, and HDLs. Patients with HTGL deficiency present with hypercholesterolemia and hypertriglyceridemia and accumulation of β-VLDLs, chylomicron remnants, IDLs and TG-rich LDLs and HDLs.

Principal for Measurement
LPL and HTGL act on a natural substrate, diglyceride to liberate monoglyceride. This is hydrolyzed by monoglyceride lipase (absolutely high specificity for monoglyceride) into glycerol and free fatty acid. Glycerol kinase acts on glycerol to glycerol-3-phosphate, which is in turn acts on by glycerol-3-phosphate oxidase to generate hydrogen peroxide.

Peroxidase converts the hydrogen peroxide, 4-Aminoantipyrine and TOOS(N-ethyl-N-(2-hydroxy-3-sulfopropyl)-m-toluidine) into quinoneimine dye. The rate of
formation of the dye, measured as an increase in absorbance at 550nm, is proportional to the lipase.

LPL requires apolipoprotein CII (apoCII) for the lipase activity, in contrast HTGL. The both lipase activities with apoCII and without apoCII are measured with 2 channel of autoanalyzer.

LPL and HTGL activities can be calculated the difference between with apoCII and without apoCII.

Features
- LPL and HTGL activities can be measured simultaneously.
- Excellent reproducibility can be obtained with an easy operation.
- No disturbance caused by co-existing substances.  
- It can be applied for standard automated machines.

Measuring Samples: 
EDTA-plasma (Postheparin)

Measurement range: 
HTGL activity: 135 ~ 431 U/L
LPL activity: 30 ~ 153 U/L
(Used Tokyo Boeki Biolis24iP type automated analyzer)
Please note: Internal testing method is applied.

Sensitivity: 
With R1A , the increase of Optical Density (OD) of reagent blank should be less than 5mAbs, and the difference between calibrator and reagent blank should be over than 20mAbs.
With R1B , the increase of Optical Density (OD) of reagent blank should be less than 5mAbs, and the difference between calibrator and reagent blank should be over than 28mAbs.

Control set is included in this kit.
Please refer to the following product if you don't need the control set.
#27185 LPL/HTGL Activity Assay Kit - IBL


Keywords:
Lipase / Lipid Metabolism / Dyslipidemia

 

FAQ

  • Question
    Q.How many samples can be measured?
  • Answer
    A.It depends on type of automated analyzers.
    For example, 50 samples can be measured using by Tokyo Boeki Biolis24iP type automated analyzer.

Note: This product is for research use only. It cannot be used as a diagnostic tool or drug.