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#27706 Mouse/Rat Intact Proinsulin Assay Kit - IBL

  • #27706 Mouse/Rat Intact Proinsulin ELISA Kit
  • #27706 Mouse/Rat Intact Proinsulin ELISA Kit
  • #27706 Mouse/Rat Intact Proinsulin ELISA Kit
  • #27706 Mouse/Rat Intact Proinsulin ELISA Kit
  • #27706 Mouse/Rat Intact Proinsulin ELISA Kit
  • #27706 Mouse/Rat Intact Proinsulin ELISA Kit
  • #27706 Mouse/Rat Intact Proinsulin ELISA Kit
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Intended Use:
Research reagents
Measuring Method:
ELISA
Sample Types:
Mouse, Rat
Measuring Samples:
Serum, EDTA-plasma, Cell culture supernatant
Measurement Range:
1.4 - 90 pg/mL (0.16 - 10 pmol/L)
Package Size1:
96 Well

※ The product indicated as "Research reagents" in the column Intended Use cannot be used
for diagnostic nor any medical purpose.

Product Overview

Product Code 27706
Product Name Mouse/Rat Intact Proinsulin Assay Kit - IBL
Maker Name Immuno-Biological Laboratories Co., Ltd.
Intended Use Research reagents
Measuring Method ELISA
Conjugate HRP
Species Mouse, Rat
Measuring Samples Serum, EDTA-plasma, Cell culture supernatant
Measurement Range 1.4 - 90 pg/mL (0.16 - 10 pmol/L)
Primary Reaction Overnight at 2 ~ 8°C
Secondary Reaction 60 minutes at 2 ~8°C
Sensitivity 0.68 pg/mL (0.076 pmol/L)
Specificity Substance Cross Reactivity
Mouse Insulin   0.1%
Storage Condition 2~8°C
Poisonous and Deleterious Substances Not Applicable
Cartagena Not Applicable
Measuring Service Available
Package Size 1 96 Well

Product Description

Proinsulin is a precursor of insulin that is synthesized in the β cells of the islet of Langerhans of pancreas. 
Most of the proinsulin synthesized in the rough endoplasmic reticulum of β cells is stored in the secretory granules. Proinsulin is cleaved by prohormone converting enzyme (PC) 1/3 at the junction of the C-peptide with the B-chain of insulin, subsequently it is cleaved by PC2 at the junction of the C-peptide with the A-chain of insulin, consequently it is divided into insulin that consists of 21 amino-acid A chain and 30 amino-acid β chain and c-peptide that consists of 33 amino acid. 
It has been considered that the conversion rate from proinsulin to insulin in type 2 diabetes patients decreases due to dropping down of PC1/3 enzymatic activity by abnormal function of pancreatic β cells. Thus, intact proinsulin level in blood or the ratio of intact proinsulin and insulin (P/I ratio) can be considered to become a potential biomarker that reflects functional change of the PC1/3 activity at pancreaticβ cell.
Especially, It has been known that the P/I ratio is a valuable pancreatic β cell evaluation method that does not require a loading test as it can be measured using blood samples in fasting. 
This ELISA kit can be used for measuring intact proinsulin, not including cleaved intermediate products, in mouse and rat samples.


Keywords:
GIP / GLP-1 / Insulin / Incretin

 

References

FAQ

  • Question
    Q.Is composition of EIA buffer of each ELISA kit all same? Can it be mixed to use?
    ELISA common FAQ
  • Answer
    A.No it isn't. As constitute of each EIA buffer is different, it cannot be mixed with other lots or EIA buffers contained in other kind of ELISA kits.
  • Question
    Q.What is the composition of concentrated wash buffer?
    ELISA common FAQ
  • Answer
    A.It contains ordinary Tween and phosphate buffer (0.05% Tween-20 in PB).
  • Question
    Q.What is the feature of the plate?
    ELISA common FAQ
  • Answer
    A.We use plate that is flat bottom and removable strip type plate (8wellx 12 strips).
  • Question
    Q.Can I re-use standard after reconstitution?
    ELISA common FAQ
  • Answer
    A.Not recommended to re-use standard after reconstitution. Please use it at once after the reconstitution.
    Please note that there are some exceptions. One time freeze-thaw the standard is acceptable for use after reconstitution for some ELISAs.
    Please check the details on each product datasheet.
  • Question
    Q.What is different between reagent blank and test sample blank?
    ELISA common FAQ
  • Answer
    A.Reagent blank means a well is only added EIA buffer and the purpose is confirming whether the measuring value is influenced by lack of washing process. Test sample blank means a well is added EIA buffer and HRP antibody and the purpose is to calculate the background.
  • Question
    Q.How many samples can be measured by this kit?
    ELISA common FAQ
  • Answer
    A.The pre-coated plate contained in our ELISA kit is 96 wells plate. We recommend to use 16 wells (2 slits) for standard and 80 wells (10 slits) for 40 samples in duplicate.
  • Question
    Q.What is LOD (Limit of Detection)?
    ELISA common FAQ
  • Answer
    A.It (LOD) is defined as sensitivity that is calculated using the NCCSL method. Please refer to a datasheet of each product.
  • Question
    Q.What is LOQ (Limit of Quantification)?
    ELISA common FAQ
  • Answer
    A.It (LOQ) is the lowest value of measurement (standard) range. Please refer to a datasheet of each product.
  • Question
    Q.Do you have any protocol for preparation of mice and/or rat brain
    tissue extracts?
    (ELISA common FAQ * Specified FAQ for ELISA that is applicable for brain tissue extract.)
  • Answer
    A.CHAPS is recommended for extractin of wild type (W.T.) mice or rat brain tissue.
    Please refer to the following link for the brain tissue extraction process using CHAPS.
    http://www.ibl-japan.co.jp/files/topics/4944_ext_02_0.pdf

    Guanidine solution is recommended for extraction of Transgenic (Tg) mice or rat brain tissue.
    Please refer to the following link and the following two scientific articles for brain tissue extraction process using Guanidine.
    http://www.ibl-japan.co.jp/files/topics/4945_ext_02_0.pdf

    Presenilin-2 mutation causes early amyloid accumulation and memory impairment in a transgenic mouse model of Alzheimer's disease. Toda T et al. J Biomed Biotechnol. 2011;2011:617974.
    https://www.ncbi.nlm.nih.gov/pubmed/?term=21234330

    Methylene blue modulates β-secretase, reverses cerebral amyloidosis, and improves cognition in transgenic mice. Mori T et al. J Biol Chem. 2014 Oct 31;289(44):30303-17.
    https://www.ncbi.nlm.nih.gov/pubmed/?term=25157105

    * The ELISA kit #27712 Aβ42 and #27714 Aβ40 have been upgraded to #27719 Aβ (1-42)(FL) and #27718 Aβ (1-40)(FL).

Note: This product is for research use only. It cannot be used as a diagnostic tool or drug.