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#27706 Mouse/Rat Intact Proinsulin Assay Kit - IBL

  • #27706 Mouse/Rat Intact Proinsulin ELISA Kit
  • #27706 Mouse/Rat Intact Proinsulin ELISA Kit
  • #27706 Mouse/Rat Intact Proinsulin ELISA Kit
  • #27706 Mouse/Rat Intact Proinsulin ELISA Kit
  • #27706 Mouse/Rat Intact Proinsulin ELISA Kit
  • #27706 Mouse/Rat Intact Proinsulin ELISA Kit
  • #27706 Mouse/Rat Intact Proinsulin ELISA Kit
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Intended Use:
Research reagents
Measuring Method:
ELISA
Sample Types:
Mouse, Rat
Measuring Samples:
Mouse serum and EDTA-plasma Rat serum and EDTA-plasma Cell culture supernatant
Measurement Range:
14.1 ~ 900 pg/mL (0.16 ~ 10 pmol/L)
Package Size1:
96 Well

Product Overview

Product Code 27706
Product Name Mouse/Rat Intact Proinsulin Assay Kit - IBL
Maker Name Immuno-Biological Laboratories Co., Ltd.
Intended Use Research reagents
Measuring Method ELISA
Conjugate HRP
Species Mouse, Rat
Measuring Samples Mouse serum and EDTA-plasma Rat serum and EDTA-plasma Cell culture supernatant
Measurement Range 14.1 ~ 900 pg/mL (0.16 ~ 10 pmol/L)
Primary Reaction Overnight at 2 ~ 8°C
Secondary Reaction 60 minutes at 2 ~8°C
Sensitivity 6.83 pg/mL (0.076 pmol/L)
Specificity Substance Cross Reactivity
Mouse Insulin   0.1%
Storage Condition 2~8°C
Poisonous and Deleterious Substances Not Applicable
Cartagena Not Applicable
Measuring Service Available
Package Size 1 96 Well

Product Description

Proinsulin is a precursor of insulin that is synthesized in the β cells of the islet of Langerhans of pancreas. 
Most of the proinsulin synthesized in the rough endoplasmic reticulum of β cells is stored in the secretory granules. Proinsulin is cleaved by prohormone converting enzyme (PC) 1/3 at the junction of the C-peptide with the B-chain of insulin, subsequently it is cleaved by PC2 at the junction of the C-peptide with the A-chain of insulin, consequently it is divided into insulin that consists of 21 amino-acid A chain and 30 amino-acid β chain and c-peptide that consists of 33 amino acid. 
It has been considered that the conversion rate from proinsulin to insulin in type 2 diabetes patients decreases due to dropping down of PC1/3 enzymatic activity by abnormal function of pancreatic β cells. Thus, intact proinsulin level in blood or the ratio of intact proinsulin and insulin (P/I ratio) can be considered to become a potential biomarker that reflects functional change of the PC1/3 activity at pancreaticβ cell.
Especially, It has been known that the P/I ratio is a valuable pancreatic β cell evaluation method that does not require a loading test as it can be measured using blood samples in fasting. 
This ELISA kit can be used for measuring intact proinsulin, not including cleaved intermediate products, in mouse and rat samples.

 

References

FAQ

  • Question
    Q.Is composition of EIA buffer of each ELISA kit all same? Can it be mixed to use?
  • Answer
    A.No it isn't. As constitute of each EIA buffer is different, it cannot be mixed with other lots or EIA buffers contained in other kind of ELISA kits.
  • Question
    Q.What is the composition of concentrated wash buffer?
  • Answer
    A.It contains ordinary Tween and phosphate buffer (0.05% Tween-20 in PB).
  • Question
    Q.What is the feature of the plate?
  • Answer
    A.We use plate that is flat bottom and removable strip type plate (8wellx 12 strips).
  • Question
    Q.Can I re-use standard after reconstitution?
  • Answer
    A.Not recommended to re-use standard after reconstitution. Please use it at once after the reconstitution.
  • Question
    Q.What is different between reagent blank and test sample blank?
  • Answer
    A.Reagent blank means a well is only added EIA buffer and the purpose is confirming whether the measuring value is influenced by lack of washing process. Test sample blank means a well is added EIA buffer and HRP antibody and the purpose is to calculate the background.
  • Question
    Q.How many samples can be measured by this kit?
  • Answer
    A.The pre-coated plate contained in our ELISA kit is 96 wells plate. We recommend to use 16 wells (2 slits) for standard and 80 wells (10 slits) for 40 samples in duplicate.

Note: This product is for research use only. It cannot be used as a diagnostic tool or drug.