Product Search

#27812 Human Phospho Tau(181P) Assay Kit - IBL

  • #27812 Human Phospho Tau(181P) ELISA Kit
  • #27812 Human Phospho Tau(181P) ELISA Kit
  • #27812 Human Phospho Tau(181P) ELISA Kit
  • #27812 Human Phospho Tau(181P) ELISA Kit
  • 4908_ext_08_en_0_S.jpg
  • 4908_ext_08_en_1_S.jpg
  • 4908_ext_08_en_6_S.jpg
  • 4908_ext_08_en_2_S.jpg
Intended Use:
Research reagents
Measuring Method:
Sample Types:
Measuring Samples:
Measurement Range:
3.6 - 230 pg/mL
Package Size1:
96 Well

※ The product indicated as "Research reagents" in the column Intended Use cannot be used
for diagnostic nor any medical purpose.

Product Overview

Product Code 27812
Product Name Human Phospho Tau(181P) Assay Kit - IBL
Maker Name Immuno-Biological Laboratories Co., Ltd.
Intended Use Research reagents
Measuring Method ELISA
Conjugate HRP
Species Human
Measuring Samples CSF
Measurement Range 3.6 - 230 pg/mL
Primary Reaction Overnight at 2 - 8°C
Secondary Reaction 30 minutes at 2 - 8°C
Sensitivity 3.06 pg/mL (Calculated by 2SD methods using the standard.)
Specificity Specifically detect Human phospho Tau p181 in human CSF.
Storage Condition 2 - 8°C
Poisonous and Deleterious Substances Not Applicable
Cartagena Not Applicable
Measuring Service Available
Package Size 1 96 Well

Product Description

Tau protein is a 45-50kDa microtubule-associated protein which consists of 352-441 amino acids. It has a role of stabilizing microtubules that transport intracellular substances in nerve cells.
It has been known that tauopathy such as Alzheimer type dementia (AD) and frontotemporal lobar degeneration (FTLD) is developed due to abnormal accumulation of Tau.
3 features of AD, senile plaque, neurofibrillary tangle and neuronal cell death have been observed in the brain of AD patients and it has been considered that neurofibrillary tangle is triggered by abnormal accumulation of Tau.
The main element of neurofibrillary tangle is called PHF (paired helical filament) and it is accumulated in nerve cells.
Overexpressed phosphorylated tau is aggregated and it becomes a main cause of PHF.
It has been thought that GSK-3β is involved in the process of phosphorylation of tau and Aβ promotes the activity for phosphorylation of tau.
It has been observed that the concentration of Tau is very high in CSF of patients with neurodegenerative diseases such as Alzheimer's disease as it is released in CSF due to neuron is destroyed while it is rarely detected in CSF of healthy subjects.
This ELISA can quantitatively measure human phosphorylated Tau (181) in CSF.

The control set is available for sale on request. Learn More

Tau, p Tau, pTau, Phospho Tau


  • Question
    Q.Is composition of EIA buffer of each ELISA kit all same? Can it be mixed to use?
    ELISA common FAQ
  • Answer
    A.No it isn't. As constitute of each EIA buffer is different, it cannot be mixed with other lots or EIA buffers contained in other kind of ELISA kits.
  • Question
    Q.What is the composition of concentrated wash buffer?
    ELISA common FAQ
  • Answer
    A.It contains ordinary Tween and phosphate buffer (0.05% Tween-20 in PB).
  • Question
    Q.What is the feature of the plate?
    ELISA common FAQ
  • Answer
    A.We use plate that is flat bottom and removable strip type plate (8wellx 12 strips).
  • Question
    Q.Can I re-use standard after reconstitution?
    ELISA common FAQ
  • Answer
    A.Not recommended to re-use standard after reconstitution. Please use it at once after the reconstitution.
    Please note that there are some exceptions. One time freeze-thaw the standard is acceptable for use after reconstitution for some ELISAs.
    Please check the details on each product datasheet.
  • Question
    Q.What is different between reagent blank and test sample blank?
    ELISA common FAQ
  • Answer
    A.Reagent blank means a well is only added EIA buffer and the purpose is confirming whether the measuring value is influenced by lack of washing process. Test sample blank means a well is added EIA buffer and HRP antibody and the purpose is to calculate the background.
  • Question
    Q.How many samples can be measured by this kit?
    ELISA common FAQ
  • Answer
    A.The pre-coated plate contained in our ELISA kit is 96 wells plate. We recommend to use 16 wells (2 slits) for standard and 80 wells (10 slits) for 40 samples in duplicate.
  • Question
    Q.What is LOD (Limit of Detection)?
    ELISA common FAQ
  • Answer
    A.It (LOD) is defined as sensitivity that is calculated using the NCCSL method. Please refer to a datasheet of each product.
  • Question
    Q.What is LOQ (Limit of Quantification)?
    ELISA common FAQ
  • Answer
    A.It (LOQ) is the lowest value of measurement (standard) range. Please refer to a datasheet of each product.
  • Question
    Q.Do you have any protocol for preparation of mice and/or rat brain
    tissue extracts?
    (ELISA common FAQ * Specified FAQ for ELISA that is applicable for brain tissue extract.)
  • Answer
    A.CHAPS is recommended for extractin of wild type (W.T.) mice or rat brain tissue.
    Please refer to the following link for the brain tissue extraction process using CHAPS.

    Guanidine solution is recommended for extraction of Transgenic (Tg) mice or rat brain tissue.
    Please refer to the following link and the following two scientific articles for brain tissue extraction process using Guanidine.

    Presenilin-2 mutation causes early amyloid accumulation and memory impairment in a transgenic mouse model of Alzheimer's disease. Toda T et al. J Biomed Biotechnol. 2011;2011:617974.

    Methylene blue modulates β-secretase, reverses cerebral amyloidosis, and improves cognition in transgenic mice. Mori T et al. J Biol Chem. 2014 Oct 31;289(44):30303-17.

    * The ELISA kit #27712 Aβ42 and #27714 Aβ40 have been upgraded to #27719 Aβ (1-42)(FL) and #27718 Aβ (1-40)(FL).

Related Products

Note: This product is for research use only. It cannot be used as a diagnostic tool or drug.