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#27402 Human Total HGF Assay Kit - MCM

Intended Use:
Research reagents
Measuring Method:
ELISA
Sample Types:
Human
Measuring Samples:
EDTA-plasma, Serum, Cell culture supernatant
Measurement Range:
78.13 ~ 5,000 pg/mL
Package Size1:
96 Well

※ The product indicated as "Research reagents" in the column Intended Use cannot be used
  for diagnostic nor any medical purpose.
※ The datasheet listed on this page is sample only. Please refer to the datasheet
  enclosed in the product purchased before use.

Product Overview

Product Overview

Product Code 27402
Product Name Human Total HGF Assay Kit - MCM
Intended Use Research reagents
Measuring Method ELISA
Conjugate HRP
Species Human
Measuring Samples EDTA-plasma, Serum, Cell culture supernatant
Measurement Range 78.13 ~ 5,000 pg/mL
Primary Reaction 60 minutes at 37 ℃
Secondary Reaction 30 minutes at 37 ℃
Sensitivity 11.00 pg/mL
Specificity Compound Cross Reactivity
Human Total HGF 100.0%
Human HAI-1 ≦0.1%
Human HAI-2 ≦0.1%
Human c-Met ≦0.1%
Human HGFA ≦0.1%
Storage Condition 2 - 8 ℃
Poisonous and Deleterious Substances Not Applicable
Cartagena Not Applicable
Measuring Service Not Available
Package Size 1 96 Well

Product Description

Product Description

Hepatocyte growth factor/scatter factor (HGF) is a liver regeneration and growth factor that was isolated and purified from the plasma of the person who suffers fulminant hepatitis. In addition blood and tissue concentrations being assessed during liver injury, increases in concentration in body fluids have been reported in other types of disease besides liver disease (e.g., inflammatory diseases, neoplastic diseases, and fibrosis). HGF is secreted as an inactive single-chain pro-form, and it exerts its physiological activity after processing by HGF activator (HGFA), coagulation factor VIIa, etc., and conversion to activated HGF, a heterodimer. This product is a kit that measures human HGF by the WHO standard.

FAQ

FAQ

  • Question
    Q.Is composition of EIA buffer of each ELISA kit all same? Can it be mixed to use?
    ELISA common FAQ
  • Answer
    A.No it isn't. As constitute of each EIA buffer is different, it cannot be mixed with other lots or EIA buffers contained in other kind of ELISA kits.
  • Question
    Q.What is the composition of concentrated wash buffer?
    ELISA common FAQ
  • Answer
    A.It contains ordinary Tween and phosphate buffer (0.05% Tween-20 in PB).
  • Question
    Q.What is the feature of the plate?
    ELISA common FAQ
  • Answer
    A.We use plate that is flat bottom and removable strip type plate (8wellx 12 strips).
  • Question
    Q.Can I re-use standard after reconstitution?
    ELISA common FAQ
  • Answer
    A.Not recommended to re-use standard after reconstitution. Please use it at once after the reconstitution.
    Please note that there are some exceptions. One time freeze-thaw the standard is acceptable for use after reconstitution for some ELISAs.
    Please check the details on each product datasheet.
  • Question
    Q.What is different between reagent blank and test sample blank?
    ELISA common FAQ
  • Answer
    A.Reagent blank means a well is only added EIA buffer and the purpose is confirming whether the measuring value is influenced by lack of washing process. Test sample blank means a well is added EIA buffer and HRP antibody and the purpose is to calculate the background.
  • Question
    Q.How many samples can be measured by this kit?
    ELISA common FAQ
  • Answer
    A.The pre-coated plate contained in our ELISA kit is 96 wells plate. We recommend to use 16 wells (2 slits) for standard and 80 wells (10 slits) for 40 samples in duplicate.
  • Question
    Q.What is LOD (Limit of Detection)?
    ELISA common FAQ
  • Answer
    A.It (LOD) is defined as sensitivity that is calculated using the NCCSL method. Please refer to a datasheet of each product.
  • Question
    Q.What is LOQ (Limit of Quantification)?
    ELISA common FAQ
  • Answer
    A.It (LOQ) is the lowest value of measurement (standard) range. Please refer to a datasheet of each product.

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