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#27820 sCLEC-2 ELISA Kit - IBL

  • 27820 sCLEC-2 ELISA Kit - IBL
  • 27820 sCLEC-2 ELISA Kit - IBL
  • 27820 sCLEC-2 ELISA Kit - IBL
Intended Use：
Research reagents
Measuring Method：
ELISA
Sample Types：
Human
Measuring Samples：
Human citrated plasma
Measurement Range：
6.25 ～ 400 pg/mL
Package Size1：
96Well

※ The product indicated as "Research reagents" in the column Intended Use cannot be used
　 for diagnostic nor any medical purpose.
※ The datasheet listed on this page is sample only. Please refer to the datasheet
　 enclosed in the product purchased before use.

Product Overview

Product Overview

Product Code 27820
Product Name sCLEC-2 ELISA Kit - IBL
Maker Name Immuno-Biological Laboratories Co., Ltd.
Intended Use Research reagents
Measuring Method ELISA
Conjugate HRP
Species Human
Measuring Samples Human citrated plasma
Measurement Range 6.25 ～ 400 pg/mL
Primary Reaction 60 minutes at 37℃
Secondary Reaction 60 minutes at 2 ～ 8℃
Sensitivity 1.47 pg/mL
Specificity Substance　　　　　　　　　Cross reactivity(%)
Human Platelet Factor 4　　　　　　＜0.1
Human β-Thrombin　　　　　　　 ＜0.1
Human P-Selectin　　 ＜0.1
Storage Condition 2 ～ 8 ℃
Poisonous and Deleterious Substances Not Applicable
Cartagena Not Applicable
Measuring Service Available
Package Size 1 96Well

Product Description

Product Description

CLEC-2 (C-type lectin-like receptor 2) is identified as the receptor for the platelet-activating snake venom, rhodocytin, and is predominantly expressed in platelets and megakaryocytes. It has been implicated in various physiological and pathological processes, including cancer metastasis and thrombosis.
Platelets are activated by factors such as collagen and shear stress when the vascular wall is damaged by trauma or inflammation. During this process, membrane-bound CLEC-2 is cleaved by platelet-derived proteases and shed into the bloodstream as soluble CLEC-2 (sCLEC-2). Furthermore, CLEC-2 is also considered to be released in association with platelet-derived microparticles generated during platelet activation, suggesting that sCLEC-2 is present in the circulation in multiple forms, including microparticle-associated forms.

#27820 sCLEC-2 ELISA Kit – IBL is available to detect both shed-form and Microparticle-associated.

It has been reported that blood levels of sCLEC-2 are increased in arterial thrombosis, such as cerebral infarction and myocardial infarction, and further studies are warranted.
Citrated plasma is recommended for measurement.

References

References

Note: Retrieve by PMID number in displayed by abstract: http://www.ncbi.nlm.nih.gov

FAQ

FAQ

  • Question
    Q.Is there any correlation with PHC's certified test reagents (STACIA)?
  • Answer
    A.A：Yes.y = 1.054x - 36.24、r = 0.975
  • Question
    Q.Are citrated plasma samples the only option?
  • Answer
    A.Please use citrated plasma.
    Citrated plasma should be collected in accordance with the “Consensus on the Handling of Specimens for Coagulation Testing” 7) by the Japanese Society of Laboratory Hematology. Plasma separation must be performed within 4 hours after blood collection, and the separated plasma must be aliquoted promptly.
  • Question
    Q.Is there any interferance by platelet?
  • Answer
    A.When platelet count in a specimen exceeds 20,000/uL, the sCLEC-2 value will exceed the 95% range of sCLEC-2 values in healthy individuals, therefore, measurements must be performed by specimens obtained in accordance with the “Consensus on the Handling of Specimens for Coagulation Testing.
  • Question
    Q.Are there any Interferance substances?
  • Answer
    A.Hemolyzed hemoglobin (up to 530 mg/dL),
    Free bilirubin (up to 5.2 mg/dL),
    Conjugated bilirubin (up to 10 mg/dL),
    Chyle (up to 1420 FTU),
    Triglyceride (up to 500 mg/dL) and
    Rheumatoid Factor (up to 250 IU/mL)
    have no influence on the assay results.

    Measurement values may be lower with high bilirubin samples. Please dilute appropriately.
  • Question
    Q.Can EDTA Plasma be measured？
  • Answer
    A.We do not recommend EDTA plasma. Higher value are observed in a certain number of cases.
  • Question
    Q.What is the recommended sample dilution factor?
  • Answer
    A.Samples should be diluted 4- to 8-fold using the dilution buffer provided with the kit. If the measured values exceed the measurement range, additional dilution should be performed as necessary.
  • Question
    Q.Is composition of EIA buffer of each ELISA kit all same? Can it be mixed to use?
    ELISA common FAQ
  • Answer
    A.No it isn't. As constitute of each EIA buffer is different, it cannot be mixed with other lots or EIA buffers contained in other kind of ELISA kits.
  • Question
    Q.What is the composition of concentrated wash buffer?
    ELISA common FAQ
  • Answer
    A.It contains ordinary Tween and phosphate buffer (0.05% Tween-20 in PB).
  • Question
    Q.What is the feature of the plate?
    ELISA common FAQ
  • Answer
    A.We use plate that is flat bottom and removable strip type plate (8wellx 12 strips).
  • Question
    Q.Can I re-use standard after reconstitution?
    ELISA common FAQ
  • Answer
    A.Not recommended to re-use standard after reconstitution. Please use it at once after the reconstitution.
    Please note that there are some exceptions. One time freeze-thaw the standard is acceptable for use after reconstitution for some ELISAs.
    Please check the details on each product datasheet.
  • Question
    Q.What is different between reagent blank and test sample blank?
    ELISA common FAQ
  • Answer
    A.Reagent blank means a well is only added EIA buffer and the purpose is confirming whether the Test sample value is influenced by lack of washing process or other operations. Test sample blank means a well is added EIA buffer and HRP antibody and the purpose is to calculate the background.
  • Question
    Q.How many samples can be measured by this kit?
    ELISA common FAQ
  • Answer
    A.The pre-coated plate contained in our ELISA kit is 96 wells plate. We recommend to use 16 wells (2 slits) for standard and 80 wells (10 slits) for 40 samples in duplicate.
  • Question
    Q.What is LOD (Limit of Detection)?
    ELISA common FAQ
  • Answer
    A.It (LOD) is defined as sensitivity that is calculated using the NCCSL method. Please refer to a datasheet of each product.
  • Question
    Q.What is LOQ (Limit of Quantification)?
    ELISA common FAQ
  • Answer
    A.It (LOQ) is the lowest value of measurement (standard) range. Please refer to a datasheet of each product.
  • Question
    Q.What is the definition of Over Night (O/N) reaction?
    ELISA common FAQ
  • Answer
    A.It means that the reaction is required more than 16 hours unless otherwise specifically defined it on a datasheet of each ELISA product.
  • Question
    Q.What is the specification of quality control for ELISA product release?
    ELISA common FAQ
  • Answer
    A.The information of specification is available on individual lot specific CoA. Please contact us with your reference lot number for obtaining of specific CoA.
  • Question
    Q.What is the number (e.g. 432143214321) at the edge of strips of the plate?
    ELISA common FAQ
  • Answer
    A.According to the plate maker (ThermoFisher), it does not have any specific meaning as it is just the number of molds.
  • Question
    Q.How to wash an ELISA plate?
    ELISA common FAQ
  • Answer
    A.Washing it by an auto-washer is highly recommended.
    If it is not available, please refer to the demo video (only 2 mins) using a washing bottle.
  • Question
    Q.The wells turned black during the test with the kit.
    ELISA common FAQ
  • Answer
    A.It is possible that the wells were not washed sufficiently during the washing process after the HRP-labeled antibody reaction.
    Be sure to wash the wells enough times as described in the data sheet with washing buffer of more than 350 µL.
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