Outsourcing services for immunity, cell culture, and gene engineering research!
We provide customized services in response to customer needs, drawing upon on our extensive experience and wealth of expertise in the immunity, cell culture, and gene engineering fields. Depending on the nature of the request, we sometimes conclude confidentiality agreements prior to starting the provision of services. All the steps of the antibody production process, from immunization to purification, are conducted internally. We make it our mission to meet the individual needs of each customer.
- 1. Development of Polyclonal Antibodies
- 2. Synthesizing Peptides
- 3. Binding Carrier Proteins
- 4. Development of Monoclonal Antibodies
- 5. Collecting Mice Ascites
- 6. Purifying Antibodies
- 7. Development of ELISA Systems
- 8. Immunohistochemical Staining (IHC)
- 9. Development of Labeled Antibodies
- 10. Development of Bio Columns (Antibodies, Various Proteins, etc.)
- 11. Gene Engineering
- 12. Cell Culturing
- 13. Supplying and Maintaining Cells for Experiments
- 14. Frozen Cell Storage Service
- 15. Contract in vitro testing service using PXB-cells LA
1. Development of Polyclonal Antibodies
We provide services for the development of various types of monoclonal antibodies, including purified proteins, synthesized peptides, pharmaceutical analogs, low molecular weight compounds, Host Cell Protein (HCP), and idiotype antibodies. Note* Maximum number of animals (excluding goats) is three. Please contact us if you require more than three animals for immunization.
2. Synthesizing Peptides
We provide services for synthesizing peptides used as immunogen, such as consultation on selecting an amino-acid sequence. We can develop high titer antibodies at reasonable cost because we manage the entire development process, from the synthesis of peptides to the fabrication of antibodies.
3. Binding Carrier Proteins
We bind carrier proteins to synthesized peptides used for immunogen, under the appropriate binding conditions, for the purpose of developing antibodies.
4. Development of Monoclonal Antibodies
We apply our expertise in immunity and cell cultures for developing monoclonal antibodies, including the immunization of mice and rabbits, cell fusion, and second cloning. We select reliable clones based on immunization, screenings, and in-depth communication with clients.
|Development of Monoclonal Antibodies per Immunogen
5. Collecting Mice Ascites
We collect mice ascites after we receive hybridoma (cell line) from clients. We have the ability to meet customer needs by applying our accumulated expertise and experience to collect mice ascites from hybridoma that normally considered difficult to work with. We can look after 10 to 1,000 mice.
|Per BALB/C Mouse
|Per Nude Mouse
|Per SCID Mouse
6. Purifying Antibodies
We provide services for purifying antibodies, such as affinity chromatography from various antiserums, ascites, fusion cell culture supernatants, and gel filtration. We can handle bulk purifying antibodies in quantities of more than 1,000 mg.
(Protein A or Protein G)
|From IgG ( up to 50 mg) to F( ab' )2 Purification
(Pepsin Decomposition / Gel Filtration)
|From IgG F (ab’)2 (up to 20 mg) to Fab’ Purification
(Reduction / Gel Filtration)
|From IgG (up to 20 mg) to Fab Purification
(Papain Decomposition, FC Removal / Gel Filtration)
7. Development of ELISA Systems
We develop ELISA systems by utilizing our know-how in commercializing IBL ELISA kits, managing the entire process from antibody development to the establishment of the ELISA system. We can also establish ELISA systems using antibodies held by customers. We plan and propose the appropriate assay design, such as the sandwich method or competition method, to fit the intended purpose. We work hard to establish ELISA system that offer outstanding performance, such as making it easy to draw the appropriate standard curve and obtain excellent recovery measurement values. Please contact us for further details.
8. Immunohistochemical Staining (IHC)
We provide immunohistochemical staining (IHC) services for research purposes. We conduct HE staining and IHC on the behalf of customers, and can also develop and stain cell block arrays. Please contact us for further details.
9. Development of Labelled Antibodies
We provide services for the development of labelled antibodies. We can label compounds (such as Biotin and FITC) to proteins and antibodies. The binding method and binding ratio are always discussed prior to the provision of service. We can also perform labeling after Fab’ fraction or F(ab’)2. Please contact us for further details.
10. Development of Bio Columns (Antibodies and Proteins, etc)
We provide services for developing bio columns that bind antibodies or proteins for the purposes of extracting or purifying bio materials. Please contact us for further details.
11. Gene Engineering
We provide gene engineering services.
12. Cell Culturing
We provide services for culturing bulk quantities of various types of cell lines. We always discuss the culturing method, collection method, and supernatant concentration with the customer prior to the provision of service. We can also handle low endotoxins for in vivo or in vitro experiments. Please contact us for further details.
13. Supplying and Maintaining Cells for Experiments
We provide services for maintaining customer cells (such as cancer cells, normal cells, and gene introduced cells) and supplying cells. We can accommodate requests for quantities as small as five T-25 flasks. Please contact us for further details.
14. Frozen Cell Storage Service
We provide services for freezing and storing cells from customers. The cells are stored frozen in liquid nitrogen (gas phase) under strict control. Please contact us for further details.
15. Contract in vitro testing service using PXB-cells LA
PXB-cells LA (manufactured by PhoenixBio Co., Ltd.) is a fresh human hepatocyte isolated from PXB mice (for lipid research).
Human fatty liver model and normal human liver one using PXB-cells LA can be used for lipid metabolism research, drug screening, and in vitro research of functional substances.
IBL provides comprehensive testing services as follows.
- ✓Cell culture of PXB-cells LA
- ✓Applying testing materials
- ✓Collect supernatant and/or cells
- ✓Initial screening
- ✓Detailed analysis
Testing samples are needed to be sent to our testing lab located in Japan and the shipping cost is covered by you.