Customized Services related FAQ

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99999
Collecting Mice Ascites
  • Question
    Q.What are the terms and conditions regarding the cell line after all the processes are finished?
  • Answer
    A.Customers can choose to return the cell line to the customer, have it stored at our laboratory, or dispose it. A storage agreement must be concluded if you wish to have us store the cell line at our laboratory.
  • Question
    Q.Is it possible to use the service even if the number of mice is less than 10?
  • Answer
    A.Yes, but there an additional charge (30,000 yen) will be applied.
  • Question
    Q.What is the volume of ascites collected?
  • Answer
    A.The average volume is around 2 mg/mL (2mL) per mouse.
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Cell Culturing
  • Question
    Q.Can you measure the concentration of cells in a cell culture supernatant?
  • Answer
    A.Yes. Please contact us for further details.
  • Question
    Q.What are the terms and conditions regarding the cell line after all the processes are finished?
  • Answer
    A.Customers can choose to return the cell line to the customer, have it stored at our laboratory, or dispose it. A storage agreement must be concluded if you wish to have us store the cell line at our laboratory.
  • Question
    Q.What are the terms and conditions for accepting cells at your laboratory?
  • Answer
    A.We need to confirm whether the cells have undergone the mycoplasma negative test before we accept them.
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Development of Monoclonal Antibodies
  • Question
    Q.How many mouse is used in development?
  • Answer
    A.Four mice are used.
  • Question
    Q.Is it possible to obtain an established cell line?
  • Answer
    A.Yes, we deliver all the established cell lines to customers.
  • Question
    Q.Is it possible to send cell culture supernatants that are not yet finished?
  • Answer
    A.Yes, unfinished supernatants can be sent.　Please contact us to learn more.
  • Question
    Q.Can you develop a kit reagent using the developed antibodies?
  • Answer
    A.Yes. Please contact us for further details.
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Development of Labeled Antibodies
  • Question
    Q.Can you label commercialized antibodies?
  • Answer
    A.Yes. However, in some cases we need to purify the antibodies.
  • Question
    Q.How do the antibodies behave after labeling?
  • Answer
    A.There are times we have observed a decline in the number of antibodies. It depends on the antibodies. We recommend you conduct tests with small quantities of antibodies.
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Development of Polyclonal Antibodies
  • Question
    Q.How many times is immunization conducted?
  • Answer
    A.It is conducted a maximum of eight times.
  • Question
    Q.What is the immunization cycle?
  • Answer
    A.Once every two weeks.
  • Question
    Q.What amount of immunogen is required for rabbits?
  • Answer
    A.Approximately 3 mg is required.
  • Question
    Q.How much immunogen is required for mice or rats?
  • Answer
    A.Approximately 1.5 mg is required.
  • Question
    Q.Is it possible to develop antibodies even if the protein is insoluble?
  • Answer
    A.Yes. Please contact us for further details.
  • Question
    Q.Is it possible to develop antibodies even if the molecular weight is small?
  • Answer
    A.Yes. Please contact us for further details.
  • Question
    Q.Can you develop a kit reagent using the developed antibodies?
  • Answer
    A.Yes. Please contact us for further details.
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Synthesizing Peptides
  • Question
    Q.Is it possible to select specific amino-acid sequences?
  • Answer
    A.Yes. Please send us the amino-acid sequences you would like.
  • Question
    Q.Is it possible to perform modifications, such as phosphorylation?
  • Answer
    A.Yes. Please send us the amino-acid sequences you would like.
  • Question
    Q.What is the purity level?
  • Answer
    A.The purity level is greater than 90%.
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Development of ELISA Systems
  • Question
    Q.Can you use commercialized antibodies to construct the ELISA system?
  • Answer
    A.Yes. However, in some cases we need to purify the antibodies.
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Purifying Antibodies
  • Question
    Q.How much antibody can be collected in affinity?
  • Answer
    A.Approximately 2 to 20 mg IgG from 20 mL of antiserum.
  • Question
    Q.Can you adjust the concentration?
  • Answer
    A.Yes we can.　Please contact us for further details.
  • Question
    Q.What is the volume of antibodies that can be collected when protein A is used for purification?
  • Answer
    A.Approximately 10 mg IgG from 1 mL of antiserum.