IBL News

Any IBL product introduced in this IBL news is applicable for research use only and it cannot be used for diagnosis or medical purpose.

A research group led by Dr. James L. Kirkland, Department of Internal Medicine, Division of General Medicine, Mayo Clinic, conducted 1) experiments using various primary human cells co-cultured with culture supernatants of senescent and non-senescent cells.
They conducted 2) an experiment in which senolytics was administered to aged mice, obese mice, and mice transplanted with senescent cells.
They also conducted 3) a study in which a combination of senolytics and drug was administered to patients with idiopathic pulmonary fibrosis. 

In these, they assessed α-Klotho expression in cells and/or urinary α-Klotho in mice and humans.

As a result, 1) α-Klotho expression was decreased in various non-senescent cells exposed to secretome from senescent cells, and 2) in young mice transplanted with senescent cells, α-Klotho expression was decreased in the brain and in the urine.
They found that in aged mice, diet-induced obese mice, and mice transplanted with senescent cells, selective genetic and pharmacological depletion of senescent cells increased α-Klotho levels in the urine and in the kidney and brain in these mice. 
And they reported 3) that administration of a combination of senolytics and drug to human patients with idiopathic pulmonary fibrosis restored urinary α-Klotho levels.

(Article)
Zhu Y et al. Orally-active, clinically-translatable senolytics restore α-Klotho in mice and humans. EBioMedicine. 2022 Mar;77:103912.

(Background)　
 α-Klotho has been studied as an anti-aging protein that may help mitigate the deleterious changes and diseases associated with aging.
Indeed, decreased α-Klotho has been reported as a hallmark of various diseases and aging.
Previous nonclinical studies have suggested that boosting α-Klotho expression may have therapeutic potentials, but no practical means of increasing α-Klotho levels were known.

In this report, it is noteworthy that orally ingestible senolytics improved aging and restored α-Klotho levels in mice and humans. 

In this paper, our ELISA kits below are used to measure α-Klotho in mouse and human urine.

#27601 Mouse soluble α-Klotho Assay Kit - IBL
#27998 Human soluble α-Klotho Assay Kit - IBL 

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