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Product News2024/01/04
Serum Klotho levels were measured using our ELISA kit in the National Health and Nutrition Examination Survey (NHANES).

Any IBL product introduced in this IBL news is applicable for research use only and it cannot be used for diagnosis or medical purpose.
Serum Klotho levels were measured using our ELISA kit in the National Health and Nutrition Examination Survey (NHANES).
The National Health and Nutrition Examination Survey (NHANES) is a semi-annual survey conducted by the Centers for Disease Control and Prevention (CDC) to monitor the health and nutritional status of the U.S. population.
On this survey, the CDC selected our ELISA kit below to measure serum Klotho levels in a total of more than 13,000 samples collected between 2007 and 2016.

The results of the measurements on Klotho were published in April 2021.
Secondary use of the published data is permitted, and after publication, third-party statistical analyses have been conducted, and as of September 2023, 54 articles have been published.
These articles provide statistical analysis of the correlation or association between serum Klotho concentrations and various components of the study subjects, such as disease, mortality, and diets. The following is a list of the subject items (including correlations and associations under limited conditions) for which correlations or associations were found for each study subject for the 54 articles.


Category Disease Correlation or Association Factors PMID
Disease Inflammation Uric acid, CRP, White blood cell, Mean platelet volume (MPV) 35603960
Cancer Cancer prevalence, Estradiol concentration, Testosterone concentration 35841322
Hypertension Hypertension prevalence (post-menopausal women) 37528365
Hyperlipidemia Hyperlipidemia prevalence, Triglyceride concentration 37641103
Hyperuricemia Hyperuricemia prevalence (Middle age - Elderly) 36411464
Osteoporosis Osteoporosis prevalence (post-menopausal women), Total bone mineral density, Thoracic bone mineral density, Lumbar bone mineral density, Trunk bone mineral density 37452417
Cardiovascular Disease,  Heart Failure Congestive heart failure prevalence, Myocardial infarction prevalence, Cardiovascular disease risk factor (BMI, Smoking, Alcohol consumption, Triglyceride, Total cholesterol), ApoB concentration 36351541*
Diabetes Diabetes prevalence, Glomerular filtration rate: GFR (Type 2 Diabetes), HbA1c (Type 2 Diabetes) 36440221
Arteriosclerosis Pulse pressure 35189169*
Abdominal aortic calcification Abdominal aortic calcification prevalence 35091971*
Frail Physical frailty phenotype, Frailty indices 37001558*
Chronic kidney disease (CKD) eGFR, Chronic kidney disease prevalence 36276390
Urinary albumin excretion Urinary albumin/creatinine ratio 36675565
Visceral fat Sagittal abdominal diameter (SAD), Visceral fat accumulation index 36555992
Obesity Obesity prevalence (Women) 36061981
Metabolic Syndrome Metabolic syndrome (MetS) prevalence 35660935*
Non-alcoholic fatty liver disease (NAFLD) Non-alcoholic fatty liver disease (NAFLD) severity 37286168
Anemia Hemoglobin, Red blood cell, Red blood cell distribution width 36797683
Depression Depression prevalence (Women) 37236270
Cognitive disorder Consortium to establish a registry for Alzheimer's disease (CERAD), Digit symbol substitution test (DSST) 37560310
Oral Health Oral health, Periodontitis severity 36204099
Mortality Adult (Age 40-79) All-cause mortality, Heart disease mortality, Cancer mortality 34628493
Rheumatoid arthritis All-cause mortality 37587536
Hypertension All-cause mortality 36457804
Diabetes All-cause mortality 35738895*
Nutrition Antioxidant diet Dietary antioxidant index 36798687
Anti-inflammatory diet Dietary inflammatory index (DII) 35761232
Nutrition Intake of carbohydrate, Alcohol consumption (inverse correlation), Total carbohydrate, Fiber, Niacin 37324910
Diet Pattern Mediterranean diet 36235560
Diet Balance Diet index 37375648
Substance Concentration Testosterone (Men) Testosterone, Estradiol, Sex hormone binding globulin, Testosterone/Estradiol ratio, Low testosterone, CRP 35820842
Serum Manganese Serum manganese 36970731
Vitamin B12 Vitamin B12 36221417
Toxic Substance,  Environment Pollution Heavy Metal Serum lead (Pb) 35753483*
Polycyclic Aromatic Hydrocarbons Urinary polycyclic aromatic hydrocarbons 35287592
PFAS Serum perfluoroalkyl, Serum polyfluoroalkyl 37228732
Environment Substance (perchlorate, nitrate, thiocyanate) Urinary thiocyanate 36096772
p-Dichlorobenzene Urinary 2,5-dichlorophenol 36309252*
Other Sleep Sleep duration 34955652
*Articles not open access.

Serum Klotho levels have been shown to be associated with as many as 21 diseases.
The association with mortality and blood levels of the substance has also been demonstrated.
Furthermore, it has been shown that diet, exposure to toxic substances/environmental pollution, and sleep may affect serum Klotho levels.
Soluble Klotho circulating in the blood is thought to inhibit the development of aging-related diseases by controlling inflammation, oxidative stress, and fibrosis, but the details of its mechanism of action remain unclear and are under active investigation.
In addition, the development of therapies to increase Klotho levels, such as drugs that increase the level of Klotho, protein drugs that administer Klotho itself, and gene therapy, is also underway.
We hope that our Klotho-related products will be useful in your research.

Please feel free to contact us.

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Immuno-Biological Laboratories Co., Ltd.
Email: do-ibl@ibl-japan.co.jp

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