#10385 Anti- Smad2L/3L (T220/T179 Phosphorylated) (1A1) Mouse IgG MoAb

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Intended Use：: Research reagents

Application：: WB, IP, IHC

Package Size1：: 50 μg

Package Size2：: 5 μg

Note on Application Abbreviations: WB:Western Blotting; IP:Immunoprecipitation; IHC:Immunohistochemistry

※ The product indicated as "Research reagents" in the column Intended Use cannot be used
　 for diagnostic nor any medical purpose.
※ The datasheet listed on this page is sample only. Please refer to the datasheet
　 enclosed in the product purchased before use.

Product Overview

Product Code	10385
Product Name	Anti- Smad2L/3L (T220/T179 Phosphorylated) (1A1) Mouse IgG MoAb
Intended Use	Research reagents
Application	WB, IP, IHC
Species	Human
Immunizing antigen	Synthetic peptide of phosphorylated Smad2L/3L (T220/T179)
Source	Mouse-Mouse hybridoma (X63 - Ag 8.653 × BALB/c mouse spleen cells)
Clone Name	1A1
Subclass	IgG1
Purification Method	Affinity purified with antigen peptide
Specificity	Reacts with phosphorylated Smad3L and Smad2L (Thr220/Thr179) of human in specific.
Package Form	Lyophilized product in PBS containing 1 % BSA and 0.05 % NaN3
Storage Condition	2 - 8℃
Poisonous and Deleterious Substances	Applicable
Cartagena	Not Applicable
Package Size 1	50 μg
Package Size 2	5 μg
Remarks1	The commercial use of products without our permission is prohibited. Please make sure to contact us and obtain permission.

Product Description

Phosphorylation of signal transduction molecules, Smad2 and Smad3, can be an important information for understanding of various biological functions of transforming growth factor (TGF)-β. TGF-β type I receptor and cyclin-dependent kinases phosphorylate both Smad2 and Smad3 at the C-terminals and at linker (middle) regions of them respectively. TGF-β signaling is mediated by Smad phosphoisoforms phophorylated at both the C-terminal and linker regions. This monoclonal antibody against for Smad2L/3L (T220/T179) recognizes the part of phosphorylated threonin (Thr220 of Smad2 and Thr179 of Smad3) located in linker region of Smad2/3 specifically. It can be used for western blotting, immunoprecipitation and immunohistochemistry. It is applicable for enzyme biochemical analysis of TGF-β signaling and makes it possible to visualize the intermolecular reaction of phosphorylated Smad signaling in human tissues and monitor them in real-time. Thus, analysis of TGF-β signaling with this antibody is expected to be widely applied to cancer serearch and fibrosis research, and to contribute to understanding the wide variety of life phenomina mediated by phosphoisoforms of Smad2/3.