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Product News2018/08/30
New Article: Influence of HAMA and requirement of SPE in measurement of active GLP-1

It has been considered that it is critical for accurate measurement of active GLP-1 in blood for basic and clinical research of diabetes.

Measuring active GLP-1 in blood without influence of non-specific reaction made by Heterophilic Antibody: HA, especially
HAMA (Human Anti-Mouse Antibodies) is required for obtaining accurate active GLP-1 value in blood and it is considered that Solid-Phase Extraction: SPE pre-treatment is required, however there is a negative aspect (disadvantage) such as time and money consuming for the pre-treatment.

In study of the following new publication, 63 human plasma samples were collected from 49 volunteers (33: normal glucose tolerance, 8: glucose tolerance, 8: type 2 diabetes) and influence of non-specific reaction by HAMA and requirement of SPE were validated using our assay kit #27784 GLP-1, active Assay Kit and other maker’s assay kit

As the result of the study, it has been suggested that our assay kit #27784 GLP-1, active Assay Kit is not affected by HAMA so that it is
NOT required SPE pre-treatment.

Please refer to the following original article for further details.
Solid-phase extraction treatment is required for measurement of active glucagon-like peptide-1 by enzyme-linked immunosorbent assay kit affected by heterophilic antibodies. Hasegawa T et al. J Diabetes Investig. 2018 Jul 11. PMID: 29993194

#27700 GLP-1, active (High sensitivity) Assay Kit is also available for sale.

Please refer to the following reference data.

Please also note that the following data is our in-house data and there is no relation with the study of the new publication above.

Reference 1(#27700 IBL without SPE vs #EGLP-35K Millipore SPE)









Reference 2 ((#27700 IBL without SPE vs #27784 IBL without SPE)









Reference 3 Assay Design #27700 / #27784









Comprehensive product lineup of incretin related products (GLP-1, GIP and Insulin) are also available for sale. Please have a look the following link for the product lineup.
Biomarker of Diabetes Incretin ( GLP-1 / GIP / Insulin) ELISA Kits


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