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Product News2020/05/07
A novel in vitro screening method for anti-lipidemic agents – Lipoprotein profile and lipid metabolism analysis using fresh human hepatocytes isolated from PXB-Mouse🄬 .

Any IBL product introduced in this IBL news is applicable for research use only and it cannot be used for diagnosis or medical purpose.

Keishi Hata et al, a senior researcher at Akita Research Institute of Food & Brewing, Akita, Japan, investigated lipid metabolism in fresh human hepatocytes isolated from PXB-Mouse🄬, a human hepatocyte chimeric mouse, conducting RT-PCR, ELISA and gel-filtration HPLC to reveal mRNA expressions and secreted protein levels and lipoprotein profile in the cultured medium. As a result, they found 1) these cells produce higher levels of lipoproteins compared to HepG2 and Huh-7, 2) produce lipoprotein mainly in VLDL fraction very similarily to human primary hepatocytes. 3) When added fenofibrate they responded to produce lesser lipoproteins in dose dependent manner, and also 4) to increase mRNA and secreted protein levels of ANGPTL4. 

(Reference)
Lipoprotein profile and lipid metabolism of PXB-cells(®), human primary hepatocytes from liver-humanized mice: proposal of novel in vitro system for screening anti-lipidemic drugs. Hata K et al. Biomed Res. 2020;41(1):33-42.

(Background)
So far researches have been made in applications of this PXB-mouse🄬 derived hepatocyte in drug metabolism and hepatitis virus infection. Contrary to these fields, applications of these cells in lipid/sugar metabolism were studied to lesser degree.

This report concludes that a combination of this new human hepatocyte and analysis of lipoprotein and lipid metabolism can be a useful method for in vitro screening of anti-lipidemic drugs.

In this report our affiliate company’s Lipoprotein profile analysis with gel-filtration HPLC (LipoSEARCH🄬) are performed and our ELISAs below are used to measure ApoB100, ANGPTL3,4,8 in cultured mediums. 

#27181 Human ApoB-100 Assay Kit – IBL
#27750 Human ANGPTL3 (highly sensitive) Assay Kit – IBL
#27749 Human ANGPTL4 Assay Kit – IBL
#27795 Human ANGPTL8 Assay Kit - IBL

We hope that we can support your research.
For more information, please feel free to contact us.

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