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#17249 Anti-Human Kappa Light Chain Goat IgG Biotin (Monkey Absorbed)

  • ELISA
Intended Use:
Research reagents
Application:
WB, IHC
Package Size1:
100 μg
Package Size2:
10 μg
Note on Application Abbreviations
WB:Western Blotting
IHC:Immunohistochemistry

※ The product indicated as "Research reagents" in the column Intended Use cannot be used
  for diagnostic nor any medical purpose.
※ The datasheet listed on this page is sample only. Please refer to the datasheet
  enclosed in the product purchased before use.

Product Overview

Product Overview

Product Code 17249
Product Name Anti-Human Kappa Light Chain Goat IgG Biotin (Monkey Absorbed)
Intended Use Research reagents
Application WB, IHC
Species Human
Immunizing antigen Purified Human Kappa light chain
Purification Method Affinity Purified with Human IgG and absorbed with Monkey IgG
Specificity Human IgG 100 %
Monkey IgG less than 0.2 %
Package Form Lyophilized product from 1 % BSA in PBS containing 0.05 % NaN3
Storage Condition 2 - 8℃
Poisonous and Deleterious Substances Applicable
Cartagena Not Applicable
Package Size 1 100 μg
Package Size 2 10 μg
Remarks1 The commercial use of products without our permission is prohibited. Please make sure to contact us and obtain permission.

Product Description

Product Description

Anti-Human IgG (goat IgG) is often used as a secondary antibody for the detection of Human IgG. Following recent advances in the development of antibody-based therapy, such as the development of Human antibodies, this secondary antibody has been used for the purpose of investigating metabolism in the living body following administration of this antibody. In studies using monkeys, conventional anti-Human IgG (goat IgG) antibody tends to cross-react with the monkey’s own antibodies and that makes it difficult to achieve highly specific detection of the administered antibody. To overcome this problem, we have incorporated the additional step of absorption with monkey IgG. This antibody is expected to minimize non-specific reaction even when it is used for ELISA with monkey blood samples, and also make it possible to detect the human antibody in a concentration range that is difficult to detect with conventional secondary antibodies.

FAQ

FAQ

  • Question
    Q.What species of monkey is used for absorbing this product?
  • Answer
    A.The crab-eating macaque (cynomolgus monkey).
  • Question
    Q.How do you biotinylation the antibody?
  • Answer
    A.NHS-LC-Biotin is used for the biotinylation.