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#17249 Anti-Human Kappa Light Chain Goat IgG Biotin (Monkey Absorbed)
- Intended Use:
- Research reagents
- Application:
- WB, IHC
- Package Size1:
- 100 μg
- Package Size2:
- 10 μg
- Note on Application Abbreviations
- WB:Western Blotting
- IHC:Immunohistochemistry
※ The product indicated as "Research reagents" in the column Intended Use cannot be used
for diagnostic nor any medical purpose.
※ The datasheet listed on this page is sample only. Please refer to the datasheet
enclosed in the product purchased before use.
Product Overview
Product Overview
Product Code | 17249 |
---|---|
Product Name | Anti-Human Kappa Light Chain Goat IgG Biotin (Monkey Absorbed) |
Intended Use | Research reagents |
Application | WB, IHC |
Species | Human |
Immunizing antigen | Purified Human Kappa light chain |
Purification Method | Affinity Purified with Human IgG and absorbed with Monkey IgG |
Specificity | Human IgG 100 % Monkey IgG less than 0.2 % |
Package Form | Lyophilized product from 1 % BSA in PBS containing 0.05 % NaN3 |
Storage Condition | 2 - 8℃ |
Poisonous and Deleterious Substances | Applicable |
Cartagena | Not Applicable |
Package Size 1 | 100 μg |
Package Size 2 | 10 μg |
Remarks1 | The commercial use of products without our permission is prohibited. Please make sure to contact us and obtain permission. |
Product Description
Product Description
Anti-Human IgG (goat IgG) is often used as a secondary antibody for the detection of Human IgG. Following recent advances in the development of antibody-based therapy, such as the development of Human antibodies, this secondary antibody has been used for the purpose of investigating metabolism in the living body following administration of this antibody. In studies using monkeys, conventional anti-Human IgG (goat IgG) antibody tends to cross-react with the monkey’s own antibodies and that makes it difficult to achieve highly specific detection of the administered antibody. To overcome this problem, we have incorporated the additional step of absorption with monkey IgG. This antibody is expected to minimize non-specific reaction even when it is used for ELISA with monkey blood samples, and also make it possible to detect the human antibody in a concentration range that is difficult to detect with conventional secondary antibodies.
FAQ
FAQ
-
Q.What species of monkey is used for absorbing this product?
-
A.The crab-eating macaque (cynomolgus monkey).
-
Q.How do you biotinylation the antibody?
-
A.NHS-LC-Biotin is used for the biotinylation.