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#18911 Anti-Human Tob (Phosphorylated) Rabbit IgG Affinity Purify

  • WB
Intended Use:
Research reagents
Application:
WB, IHC
Package Size1:
100 μg
Note on Application Abbreviations
WB:Western Blotting
IHC:Immunohistochemistry

※ The product indicated as "Research reagents" in the column Intended Use cannot be used
  for diagnostic nor any medical purpose.
※ The datasheet listed on this page is sample only. Please refer to the datasheet
  enclosed in the product purchased before use.

Product Overview

Product Overview

Product Code 18911
Product Name Anti-Human Tob (Phosphorylated) Rabbit IgG Affinity Purify
Intended Use Research reagents
Application WB, IHC
Species Human
Immunizing antigen Synthetic peptide of human Tob protein (phosphorylated)(ASSVSS(pS)P(pS)PPFGHS)
Purification Method Purified with antigen peptide
Specificity Confirmed by western blotting using transfectant cells.
Package Form Lyophilized product from 1 % BSA in PBS containing 0.05% NaN3
Storage Condition 2 - 8℃
Poisonous and Deleterious Substances Applicable
Cartagena Not Applicable
Package Size 1 100 μg
Remarks1 The commercial use of products without our permission is prohibited. Please make sure to contact us and obtain permission.

Product Description

Product Description

Tob is a member of an emerging family of genes with anti-proliferative function. Tob is rapidly phosphorylated at Ser 152, Ser 154, and Ser 164 by Erk1 and Erk2 upon growth-factor stimulation. Tob inhibits cell growth by suppressing cyclin D1 expression, which is canceled by Erk1- and Erk2-mediated Tob phosphorylation. In recent, it is reported that mutant forms of Tob, in which serines are replaced by
glutamic acids to mimic phosphorylation, show a much reduced ability to inhibit the cell cycle progression to S phase from G0/G1 phase, as compared with wild-type Tob, indicating that ERK phosphorylation negatively regulates the anti-proliferative function of Tob.

FAQ

FAQ

  • Question
    Q.Can this antibody be used for mice lysate?
  • Answer
    A.The antigen, GST-TobN that is used in the article is fusion protein of N-terminal 168 amino acids of Tob protein and GST.
    The sequence of this region is same between human and mouse.
    Therefore, theoretically it would be possible to detect using by the antibody #18911 with mice sample but as we have not tested, we cannot be certain about the point.

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