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#28045 Anti-Rat RGMa Rabbit IgG Affinity Purify

  • WB_IP
Intended Use:
Research reagents
Application:
WB, IP, FCM
Package Size1:
100 μg
Package Size2:
10 μg
Note on Application Abbreviations
WB:Western Blotting
IP:Immunoprecipitation
FCM:Flow Cytometry

※ The product indicated as "Research reagents" in the column Intended Use cannot be used
  for diagnostic nor any medical purpose.
※ The datasheet listed on this page is sample only. Please refer to the datasheet
  enclosed in the product purchased before use.

Product Overview

Product Overview

Product Code 28045
Product Name Anti-Rat RGMa Rabbit IgG Affinity Purify
Intended Use Research reagents
Application WB, IP, FCM
Species Rat
Immunizing antigen Synthetic peptide of a part of Rat RGMa (DFQAFRANAESPRR)
Purification Method Purified with antigen peptide
Specificity Reacts with rat RGMa (confirmed by western blotting with transfectant).
Package Form Lyophilized product from 1 % BSA in PBS containing 0.05 % NaN3
Storage Condition 2 - 8℃
Poisonous and Deleterious Substances Applicable
Cartagena Not Applicable
Package Size 1 100 μg
Package Size 2 10 μg
Remarks1 The commercial use of products without our permission is prohibited. Please make sure to contact us and obtain permission.

Product Description

Product Description

RGMa (Repulsive Guidance Molecule a) is a GIP-anchored protein, about 33 kDa, and it is implicated in axonal guidance and neural tube closure of embryonic retina. It has reported as a potent inhibitor of axonal regeneration in the adult central nervous system (CNS). It is speculated that RGMa inhibits neurite outgrowth by activating the RhoA-Rho kinase signaling pathway in vitro. While, it has reported that, in a rat SCI (spinal cord injury) model, neurite outgrowth is enhanced by administration of anti-RGMa antibody to lesion site, resulting they are improved in mortor functional recovery. Thus function analysis of RGMa is attracting attention in a research field of axonal regeneration of CNS. This antibody detects rat RGMa and can be used for western blotting, immuno-precipitation and FACS analysis. And it is also reported that administration of the antibody to cerebellar granule cells of 6 - 8 days-old rat, in vitro, neutralizes RGMa-dependent inhibition of neurite outgrowth. This product cannot be used for neutralization test.

FAQ

FAQ

  • Question
    Q.Why the antibody cannot be used as neutralizing antibody?
  • Answer
    A.As the catalog product contains BSA and NaN3, the antibody cannot be used as neutralizing antibody.
    However, if we specifically produce the antibody as customized product without BSA and NaN3 and sterilization for filling process in frozen, it can be used as neutralizing antibody.
    Please feel free to contact us if you are interested in the customized antibody.

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