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#28045 Anti-Rat RGMa Rabbit IgG Affinity Purify
- Intended Use：
- Research reagents
- Application：
- WB, IP, FCM
- Package Size1：
- 100 μg
- Package Size2：
- 10 μg
- Note on Application Abbreviations
- WB:Western Blotting
- IP:Immunoprecipitation
- FCM:Flow Cytometry
※ The product indicated as "Research reagents" in the column Intended Use cannot be used
for diagnostic nor any medical purpose.
※ The datasheet listed on this page is sample only. Please refer to the datasheet
enclosed in the product purchased before use.
Product Overview
Product Overview
| Product Code | 28045 |
|---|---|
| Product Name | Anti-Rat RGMa Rabbit IgG Affinity Purify |
| Intended Use | Research reagents |
| Application | WB, IP, FCM |
| Species | Rat |
| Immunizing antigen | Synthetic peptide of a part of Rat RGMa (DFQAFRANAESPRR) |
| Purification Method | Purified with antigen peptide |
| Specificity | Reacts with rat RGMa (confirmed by western blotting with transfectant). |
| Package Form | Lyophilized product from 1 % BSA in PBS containing 0.05 % NaN3 |
| Storage Condition | 2 - 8℃ |
| Poisonous and Deleterious Substances | Applicable |
| Cartagena | Not Applicable |
| Package Size 1 | 100 μg |
| Package Size 2 | 10 μg |
| Remarks1 | The commercial use of products without our permission is prohibited. Please make sure to contact us and obtain permission. |
Product Description
Product Description
RGMa (Repulsive Guidance Molecule a) is a GIP-anchored protein, about 33 kDa, and it is implicated in axonal guidance and neural tube closure of embryonic retina. It has reported as a potent inhibitor of axonal regeneration in the adult central nervous system (CNS). It is speculated that RGMa inhibits neurite outgrowth by activating the RhoA-Rho kinase signaling pathway in vitro. While, it has reported that, in a rat SCI (spinal cord injury) model, neurite outgrowth is enhanced by administration of anti-RGMa antibody to lesion site, resulting they are improved in mortor functional recovery. Thus function analysis of RGMa is attracting attention in a research field of axonal regeneration of CNS. This antibody detects rat RGMa and can be used for western blotting, immuno-precipitation and FACS analysis. And it is also reported that administration of the antibody to cerebellar granule cells of 6 - 8 days-old rat, in vitro, neutralizes RGMa-dependent inhibition of neurite outgrowth. This product cannot be used for neutralization test.
References (Not listed at PubMed)
An Antibody to RGMa Promotes Regeneration of Cochlear Synapses after Noise Exposure. Jerome Nevoux et al. bioRxiv.
References (Not listed at PubMed)
An Antibody to RGMa Promotes Regeneration of Cochlear Synapses after Noise Exposure. Jerome Nevoux et al. bioRxiv.
References
References
- Treatment With the Neutralizing Antibody Against Repulsive Guidance Molecule-a Promotes Recovery From Impaired Manual Dexterity in a Primate Model of Spinal Cord Injury. Nakagawa H et al. Cereb Cortex. 2019 Feb 1;29(2):561-572.PMID: 29315368
- Repulsive guidance molecule a blockade exerts the immunoregulatory function in DCs stimulated with ABP and LPS. Xu X et al. Hum Vaccin Immunother. 2016 Aug 2;12(8):2169-2180.PMID: 26986456
- Activated microglia inhibit axonal growth through RGMa. Kitayama M et al. PLoS One. 2011;6(9):e25234.PMID: 21957482
Note: Retrieve by PMID number in displayed by abstract: http://www.ncbi.nlm.nih.gov
FAQ
FAQ
-
Q.Why the antibody cannot be used as neutralizing antibody? -
A.As the catalog product contains BSA and NaN3, the antibody cannot be used as neutralizing antibody.
However, if we specifically produce the antibody as customized product without BSA and NaN3 and sterilization for filling process in frozen, it can be used as neutralizing antibody.
Please feel free to contact us if you are interested in the customized antibody.
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