#27131 Human G-CSF Assay Kit - IBL

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Intended Use：: Research reagents

Measuring Method：: ELISA

Sample Types：: Human

Measuring Samples：: EDTA-plasma (*1), Serum (*1), Cell culture supernatant

Measurement Range：: 7.81 ~ 500 pg/mL (1st incubation for 60 minutes at 37℃)
1.95 ~ 250 pg/mL (1st incubation for Overnight at 2 - 8℃)

Package Size1：: 96 Well

※ The product indicated as "Research reagents" in the column Intended Use cannot be used
　 for diagnostic nor any medical purpose.
※ The datasheet listed on this page is sample only. Please refer to the datasheet
　 enclosed in the product purchased before use.

Product Overview

Product Code	27131
Product Name	Human G-CSF Assay Kit - IBL
Intended Use	Research reagents
Measuring Method	ELISA
Conjugate	HRP
Species	Human
Measuring Samples	EDTA-plasma (1), Serum (1), Cell culture supernatant
Measurement Range	7.81 ~ 500 pg/mL (1st incubation for 60 minutes at 37℃) 1.95 ~ 250 pg/mL (1st incubation for Overnight at 2 - 8℃)
Primary Reaction	60minutes at 37 ℃
Secondary Reaction	30 minutes at 37 ℃
Sensitivity	1.23 pg/mL
Specificity	Compound Cross Reactivity Human G-CSF 100.0% Human GM-CSF ＜0.1 %
Storage Condition	2 - 8 ℃
Poisonous and Deleterious Substances	Not Applicable
Cartagena	Not Applicable
Measuring Service	Not Available
Package Size 1	96 Well
Remarks1	(*1) Our in-house experiment suggested that the assay in low diluted serum or EDTA - plasma samples may cause the lower values due to the interference of substances in blood. (Please see the datasheet “Table. 1.Titer Assay, PERFORMANCE CHARACTERISTICS” on page 2.)

Product Description

G-CSF is the cytokine that stimulates differentiation and propagation of neutrophil precursor cells. It is produced by fibroblast and endothelial cells and it is reported to stimulate the propagation in some tumor.

The control set is available for sale on request. Learn More

References

Note: Retrieve by PMID number in displayed by abstract: http://www.ncbi.nlm.nih.gov

FAQ

Q.Can this kit be used for mouse or rat samples?
A.No, they cannot.
Q.What is the epitope of antibodies used in this ELISA?
A.Since a whole recombinant human G-CSF was used as the immunogen for these antibodies and no epitope mapping has been done, the information is not available.
Q.Is composition of EIA buffer of each ELISA kit all same? Can it be mixed to use?
ELISA common FAQ
A.No it isn't. As constitute of each EIA buffer is different, it cannot be mixed with other lots or EIA buffers contained in other kind of ELISA kits.
Q.What is the composition of concentrated wash buffer?
ELISA common FAQ
A.It contains ordinary Tween and phosphate buffer (0.05% Tween-20 in PB).
Q.What is the feature of the plate?
ELISA common FAQ
A.We use plate that is flat bottom and removable strip type plate (8wellx 12 strips).
Q.Can I re-use standard after reconstitution?
ELISA common FAQ
A.Not recommended to re-use standard after reconstitution. Please use it at once after the reconstitution.
Please note that there are some exceptions. One time freeze-thaw the standard is acceptable for use after reconstitution for some ELISAs.
Please check the details on each product datasheet.
Q.What is different between reagent blank and test sample blank?
ELISA common FAQ
A.Reagent blank means a well is only added EIA buffer and the purpose is confirming whether the Test sample value is influenced by lack of washing process or other operations. Test sample blank means a well is added EIA buffer and HRP antibody and the purpose is to calculate the background.
Q.How many samples can be measured by this kit?
ELISA common FAQ
A.The pre-coated plate contained in our ELISA kit is 96 wells plate. We recommend to use 16 wells (2 slits) for standard and 80 wells (10 slits) for 40 samples in duplicate.
Q.What is LOD (Limit of Detection)?
ELISA common FAQ
A.It (LOD) is defined as sensitivity that is calculated using the NCCSL method. Please refer to a datasheet of each product.
Q.What is LOQ (Limit of Quantification)?
ELISA common FAQ
A.It (LOQ) is the lowest value of measurement (standard) range. Please refer to a datasheet of each product.
Q.What is the definition of Over Night (O/N) reaction?
ELISA common FAQ
A.It means that the reaction is required more than 16 hours unless otherwise specifically defined it on a datasheet of each ELISA product.
Q.What is the specification of quality control for ELISA product release?
ELISA common FAQ
A.The information of specification is available on individual lot specific CoA. Please contact us with your reference lot number for obtaining of specific CoA.
Q.What is the number (e.g. 432143214321) at the edge of strips of the plate?
ELISA common FAQ
A.According to the plate maker (ThermoFisher), it does not have any specific meaning as it is just the number of molds.
Q.How to wash an ELISA plate?
ELISA common FAQ
A.Washing it by an auto-washer is highly recommended.
If it is not available, please refer to the demo video (only 2 mins) using a washing bottle.
Q.The wells turned black during the test with the kit.
ELISA common FAQ
A.It is possible that the wells were not washed sufficiently during the washing process after the HRP-labeled antibody reaction.
Be sure to wash the wells enough times as described in the data sheet with washing buffer of more than 350 µL.
Q.How do you define the acceptance range of quality control of control sets?
Control Set Common FAQ
A.In principal, it is defined as ±15%-20% based the standard value described on each datasheet.
Q.What is the shelf life of control set?
Control Set Common FAQ
A.The shelf life is 12 months upon the goods is dispatched from our lab if the goods is unopned.
Q.What is the storage condition of control sets?
Control Set Common FAQ
A.It should be stored under chilled condition (2～10℃).
Q.Can the control set be used after reconstitution?
Control Set Common FAQ
A.No it cannot be re-used after the reconstitution.
Q.What is the package form of control sets?
Control Set Common FAQ
A.It is lyophilized form.