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#27736 Human APP770 Assay Kit - IBL

  • #27736 Human APP770 ELISA Kit
  • #27736 Human APP770 ELISA Kit
  • #27736 Human APP770 ELISA Kit
  • #27736 Human APP770 ELISA Kit
  • #27736 Human APP770 ELISA Kit
  • #27736 Human APP770 ELISA Kit
Intended Use:
Research reagents
Measuring Method:
ELISA
Sample Types:
Human
Measuring Samples:
EDTA-plasma, CSF, Cell culture supernatant
Measurement Range:
0.10 - 6.2 ng/mL
Package Size1:
96 Well

※ The product indicated as "Research reagents" in the column Intended Use cannot be used
  for diagnostic nor any medical purpose.
※ The datasheet listed on this page is sample only. Please refer to the datasheet
  enclosed in the product purchased before use.

Product Overview

Product Overview

Product Code 27736
Product Name Human APP770 Assay Kit - IBL
Intended Use Research reagents
Measuring Method ELISA
Conjugate HRP
Species Human
Measuring Samples EDTA-plasma, CSF, Cell culture supernatant
Measurement Range 0.10 - 6.2 ng/mL
Primary Reaction Overnight at 2 - 8 ℃
Secondary Reaction 30 minutes at 2 - 8 ℃
Sensitivity 0.03 ng/mL
Specificity Substance Cross-Reactivity
Human APP770 100.0%
Human APP695 < 0.1 %
Human APP751 < 0.1 %
Storage Condition 2 - 8 ℃
Poisonous and Deleterious Substances Not Applicable
Cartagena Not Applicable
Measuring Service Not Available
Package Size 1 96 Well

Product Description

Product Description

Alzheimer's disease (AD) is a typical senile dementia and it is believed that accumulation of Amyloidβ (Aβ) in brain parenchyma is a major cause of the disease. Meanwhile, accumulation of Aβ has been also observed on cerebrovascular walls of nearly 90% of individuals suffering with AD. Aβ is known to be produced from Amyloid Precursor Protein (APP) by cleavage with two types of protease (β- and γ- secretase) and Aβ molecules which accumulate in brain parenchyma are mainly produced from APP expressed in neurons, APP695. In contrast to this, another different APP, APP770, was found to be expressed in cerebrovascular endothelial cells, and it has been newly reported that the Aβ produced from the APP770 can accumulate on cerebrovascular walls. This ELISA kit can measure human APP770 in EDTA-plasma, ceribrospinal fluid and cell culture supernatant.

FAQ

FAQ

  • Question
    Q.What is the epitope of the pre-coated antibody (anti-human APP OX2 (351) Rabbit IgG A.P.)?
  • Answer
    A.The epitope is not available because the rights belong to a third party.
  • Question
    Q.Can this kit be used for mouse or rat samples?
  • Answer
    A.No, it cannot.
  • Question
    Q.Is composition of EIA buffer of each ELISA kit all same? Can it be mixed to use?
    ELISA common FAQ
  • Answer
    A.No it isn't. As constitute of each EIA buffer is different, it cannot be mixed with other lots or EIA buffers contained in other kind of ELISA kits.
  • Question
    Q.What is the composition of concentrated wash buffer?
    ELISA common FAQ
  • Answer
    A.It contains ordinary Tween and phosphate buffer (0.05% Tween-20 in PB).
  • Question
    Q.What is the feature of the plate?
    ELISA common FAQ
  • Answer
    A.We use plate that is flat bottom and removable strip type plate (8wellx 12 strips).
  • Question
    Q.Can I re-use standard after reconstitution?
    ELISA common FAQ
  • Answer
    A.Not recommended to re-use standard after reconstitution. Please use it at once after the reconstitution.
    Please note that there are some exceptions. One time freeze-thaw the standard is acceptable for use after reconstitution for some ELISAs.
    Please check the details on each product datasheet.
  • Question
    Q.What is different between reagent blank and test sample blank?
    ELISA common FAQ
  • Answer
    A.Reagent blank means a well is only added EIA buffer and the purpose is confirming whether the measuring value is influenced by lack of washing process. Test sample blank means a well is added EIA buffer and HRP antibody and the purpose is to calculate the background.
  • Question
    Q.How many samples can be measured by this kit?
    ELISA common FAQ
  • Answer
    A.The pre-coated plate contained in our ELISA kit is 96 wells plate. We recommend to use 16 wells (2 slits) for standard and 80 wells (10 slits) for 40 samples in duplicate.
  • Question
    Q.What is LOD (Limit of Detection)?
    ELISA common FAQ
  • Answer
    A.It (LOD) is defined as sensitivity that is calculated using the NCCSL method. Please refer to a datasheet of each product.
  • Question
    Q.What is LOQ (Limit of Quantification)?
    ELISA common FAQ
  • Answer
    A.It (LOQ) is the lowest value of measurement (standard) range. Please refer to a datasheet of each product.
  • Question
    Q.What is the definition of Over Night (O/N) reaction?
    ELISA common FAQ
  • Answer
    A.It means that the reaction is required more than 16 hours unless otherwise specifically defined it on a datasheet of each ELISA product.
  • Question
    Q.What is the specification of quality control for ELISA product release?
    ELISA common FAQ
  • Answer
    A.The information of specification is available on individual lot specific CoA. Please contact us with your reference lot number for obtaining of specific CoA.
  • Question
    Q.What is the number (e.g. 432143214321) at the edge of strips of the plate?
    ELISA common FAQ
  • Answer
    A.According to the plate maker (ThermoFisher), it does not have any specific meaning as it is just the number of molds.
  • Question
    Q.How to wash an ELISA plate?
    ELISA common FAQ
  • Answer
    A.Washing it by an auto-washer is highly recommended.
    If it is not available, please refer to the demo video (only 2 mins) using a washing bottle.

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