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#27768 Mouse IL-6 Assay Kit - IBL

  • 27768 Mouse IL-6 ELISA Kit
Intended Use:
Research reagents
Measuring Method:
ELISA
Sample Types:
Mouse
Measuring Samples:
EDTA-plasma, Serum, Cell culture supernatant
Measurement Range:
10.94 ~ 700 pg/mL
Package Size1:
96 Well

※ The product indicated as "Research reagents" in the column Intended Use cannot be used
  for diagnostic nor any medical purpose.
※ The datasheet listed on this page is sample only. Please refer to the datasheet
  enclosed in the product purchased before use.

Product Overview

Product Overview

Product Code 27768
Product Name Mouse IL-6 Assay Kit - IBL
Intended Use Research reagents
Measuring Method ELISA
Conjugate HRP
Species Mouse
Measuring Samples EDTA-plasma, Serum, Cell culture supernatant
Measurement Range 10.94 ~ 700 pg/mL
Primary Reaction Overnight at 2 - 8 ℃
Secondary Reaction 30 minutes at 2 - 8 ℃
Sensitivity 1.07 pg/mL
Specificity Compound Cross Reactivity
Mouse IL-6 100.0%
Mouse Leptin < 0.1 %
Mouse MIP-2 < 0.1 %
Mouse OPN < 0.1 %
Storage Condition 2 - 8 ℃
Poisonous and Deleterious Substances Not Applicable
Cartagena Not Applicable
Measuring Service Not Available
Package Size 1 96 Well

Product Description

Product Description

Interleukin-6 (IL-6) is a cytokine that was discovered as a B-cell differentiation factor. IL-6 is not only an antibody-producing system, but its physiological activities, such as induction of acute-phase protein synthesis in hepatocytes, and growth stimulation based on its synergistic effect with IL-3, etc., have attracted great interest. Associations between IL-6 and pathology have been steadily demonstrated. For example, the growth factor in several diseases has been reported to be IL-6, and myeloma cells themselves have been reported to synthesize IL-6 and express IL-6 receptors. In addition, IL-6 has been suggested to contribute to a variety of inflammatory diseases and autoimmune diseases. This kit makes it possible to detect the IL-6 in mouse blood and in the supernatant of cultured cells with high sensitivity.

The control set is available for sale on request. Learn More

FAQ

FAQ

  • Question
    Q.Is composition of EIA buffer of each ELISA kit all same? Can it be mixed to use?
    ELISA common FAQ
  • Answer
    A.No it isn't. As constitute of each EIA buffer is different, it cannot be mixed with other lots or EIA buffers contained in other kind of ELISA kits.
  • Question
    Q.What is the composition of concentrated wash buffer?
    ELISA common FAQ
  • Answer
    A.It contains ordinary Tween and phosphate buffer (0.05% Tween-20 in PB).
  • Question
    Q.What is the feature of the plate?
    ELISA common FAQ
  • Answer
    A.We use plate that is flat bottom and removable strip type plate (8wellx 12 strips).
  • Question
    Q.Can I re-use standard after reconstitution?
    ELISA common FAQ
  • Answer
    A.Not recommended to re-use standard after reconstitution. Please use it at once after the reconstitution.
    Please note that there are some exceptions. One time freeze-thaw the standard is acceptable for use after reconstitution for some ELISAs.
    Please check the details on each product datasheet.
  • Question
    Q.What is different between reagent blank and test sample blank?
    ELISA common FAQ
  • Answer
    A.Reagent blank means a well is only added EIA buffer and the purpose is confirming whether the measuring value is influenced by lack of washing process. Test sample blank means a well is added EIA buffer and HRP antibody and the purpose is to calculate the background.
  • Question
    Q.How many samples can be measured by this kit?
    ELISA common FAQ
  • Answer
    A.The pre-coated plate contained in our ELISA kit is 96 wells plate. We recommend to use 16 wells (2 slits) for standard and 80 wells (10 slits) for 40 samples in duplicate.
  • Question
    Q.What is LOD (Limit of Detection)?
    ELISA common FAQ
  • Answer
    A.It (LOD) is defined as sensitivity that is calculated using the NCCSL method. Please refer to a datasheet of each product.
  • Question
    Q.What is LOQ (Limit of Quantification)?
    ELISA common FAQ
  • Answer
    A.It (LOQ) is the lowest value of measurement (standard) range. Please refer to a datasheet of each product.

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