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#27600 Gd-IgA1 (Galactose-deficient IgA1) Assay Kit - IBL
- Intended Use:
- Research reagents
- Measuring Method:
- ELISA
- Sample Types:
- Human
- Measuring Samples:
- Serum, EDTA-plasma and Urine
- Measurement Range:
- 1.56 ~ 100 ng/mL
- Package Size1:
- 96 Well
※ The product indicated as "Research reagents" in the column Intended Use cannot be used
for diagnostic nor any medical purpose.
※ The datasheet listed on this page is sample only. Please refer to the datasheet
enclosed in the product purchased before use.
Product Overview
Product Overview
Product Code | 27600 |
---|---|
Product Name | Gd-IgA1 (Galactose-deficient IgA1) Assay Kit - IBL |
Maker Name | Immuno-Biological Laboratories Co., Ltd. |
Intended Use | Research reagents |
Measuring Method | ELISA |
Conjugate | HRP |
Species | Human |
Measuring Samples | Serum, EDTA-plasma and Urine |
Measurement Range | 1.56 ~ 100 ng/mL |
Primary Reaction | 60 minutes at room temperature |
Secondary Reaction | 30 minutes at room temperature |
Sensitivity | 0.488 ng/mL |
Storage Condition | 2~8°C |
Poisonous and Deleterious Substances | Not Applicable |
Cartagena | Not Applicable |
Measuring Service | Available |
Package Size 1 | 96 Well |
Product Description
Product Description
Galactose-deficient IgA1 (Gd-IgA1) attracts a lot of attentions as a critical effector molecule in the pathogenesis and progression of IgA nephropathy (IgAN) in recent studies. It has been suggested that several O-link glycans modified regions exist in the heavy chain hinge region of human IgA1 molecule andGd-IgA1 circulates in blood stream of the patients with the pathological condition of IgAN. The measuring system using snail (helix aspersa; HAA) lectin that is extracted from snail has been used in past numerous studies and it was revealed that serum levels of Gd-IgA1 in patients with IgAN is significantly elevated compared with the level of healthy subjects or patients with renal diseases other than IgAN. Thus, the importance and purpose of measuring serum Gd-IgA1 level have been gradually recognized from such studies.
However, since the measuring system used HAA lectin is not suitable for measuring multiple and massive samples in large scale studies due to its instability of glycan-recognizing activity, development of alternative measuring system that can quantitatively measure human Gd-IgA1 in serum with stable and reliable data has been considered as an essential and urgent matter. This IBL ELISA kit using the monoclonal antibody that specifically recognizes galactose-deficient hinge sequence of human Gd-IgA1 is a lectin non-dependent measuring system that can quantitatively measure Gd-IgA1 in human serum,which is also suitable for large scale studies because of its stability.
However, since the measuring system used HAA lectin is not suitable for measuring multiple and massive samples in large scale studies due to its instability of glycan-recognizing activity, development of alternative measuring system that can quantitatively measure human Gd-IgA1 in serum with stable and reliable data has been considered as an essential and urgent matter. This IBL ELISA kit using the monoclonal antibody that specifically recognizes galactose-deficient hinge sequence of human Gd-IgA1 is a lectin non-dependent measuring system that can quantitatively measure Gd-IgA1 in human serum,which is also suitable for large scale studies because of its stability.
References
References
- Proceedings of 16th International Symposium on IgA Nephropathy IgA Nephropathy 2021, 21–23 September 2021 (Virtual).
IgAN: A South-Asian perspective. Page 5.
What biomarkers are on the horizon that may help risk stratify patients with IgAN? Page 45.
Clinical significance of intensity of glomerular galactose-deficient IgA1 deposition in IgA nephropathy. Page 69.
Relevance of serum Gd-IgA1 levels in South Asian IgAN- prospective longitudinal cohort (GRACE-IgANI). Page 74.PMID: N/A - Clinical significance of serum galactose-deficient immunoglobulin A1 for detection of recurrent immunoglobulin A nephropathy in kidney transplant recipients. Park WY et al. Kidney Res Clin Pract. 2021 Apr 15.PMID: 33866766
- Clinical Significance of Serum Galactose-Deficient IgA1 Level in Children with IgA Nephropathy. Irabu H et al. J Immunol Res. 2020 May 21;2020:4284379.PMID: 32537466
- Biomarkers for IgA nephropathy on the basis of multi-hit pathogenesis. Suzuki H. Clin Exp Nephrol. 2019 Jan;23(1):26-31.PMID: 29740706
- Galactose deficient IgA1 (GD-IgA1) in skin and serum from patients with skin-limited and systemic IgA Vasculitis. Neufeld M et al. J Am Acad Dermatol. 2019 Mar 19. pii: S0190-9622(19)30443-8.PMID: 30902725
- Significance of serum galactose deficient IgA1 as a potential biomarker for IgA nephropathy: A case control study. Bagchi S et al. PLoS One. 2019 Mar 27;14(3):e0214256.PMID: 30917188
- Clinical Significance of Galactose-Deficient IgA1 by KM55 in Patients with IgA Nephropathy. Zhang K et al. Kidney Blood Press Res. 2019;44(5):1196-1206.PMID: 31574506
- IgA nephropathy and IgA vasculitis with nephritis have a shared feature involving galactose-deficient IgA1-oriented pathogenesis. Suzuki H et al. Kidney Int. 2018 Jan 9. pii: S0085-2538(17)30799-8.PMID: 29329643
- Galactose-Deficient IgA1-Specific Antibody Recognizes GalNAc-Modified Unique Epitope on Hinge Region of IgA1. Yamasaki K et al. Monoclon Antib Immunodiagn Immunother. 2018 Dec;37(6):252-256.PMID: 30570353
- Clinical significance of serum and mesangial galactose-deficient IgA1 in patients with IgA nephropathy. Wada Y et al. PLoS One. 2018 Nov 2;13(11):e0206865.PMID: 30388165
- Novel lectin-independent approach to detect galactose-deficient IgA1 in IgA nephropathy. Yasutake J et al. Nephrol Dial Transplant. 2015 Aug;30(8):1315-21.PMID: 26109484
Note: Retrieve by PMID number in displayed by abstract: http://www.ncbi.nlm.nih.gov
FAQ
FAQ
-
Q.Does Neuramidase treatment require for the sample?
-
A.No it doesn't. There is no much different for the result between with and without Neuramidase.
-
Q.I found the recommended dilution ratio is between 200 and 800 fold. That is quite a wide range. What is the most optimized dilution ratio?
-
A.Some samples have a very high value of Gd-IgA1 and such samples would be range over that why the recommended dilution ratio is wide.
We recommend to start from 200 fold dilution and find the best dilution ratio for your samples. The optimized dilution ratio should be determined by each user. -
Q.How much is approx. normal IgA in blood?
-
A.Total IgA is approx 1-4mg/mL in blood.
-
Q.How much is approx. normal Gd-IgA in blood?
-
A.As it is approx 1,000 ng/mL in blood, more than 200 fold dilution is recommended for measurement.
-
Q.What is the material used for the standard?
-
A.It is intact IgA1 (IgA Fab+Fc).
-
Q.Why the standard is IgA1 instead of Gd-IgA1?
-
A.As certain amount of Gd-IgA1 is also contained in normal subject blood samples and the capture antibody(KM55 monoclonal antibody) used in this kit specifically detects Gd-IgA1 so that human IgA1 is applied as the standard.
-
Q.What is the dilution ratio for urine sample?
-
A.Recommended dilution ratio is 2-4 fold using “4 EIA buffer” enclosed in this kit.
-
Q.Is composition of EIA buffer of each ELISA kit all same? Can it be mixed to use?
ELISA common FAQ -
A.No it isn't. As constitute of each EIA buffer is different, it cannot be mixed with other lots or EIA buffers contained in other kind of ELISA kits.
-
Q.What is the composition of concentrated wash buffer?
ELISA common FAQ -
A.It contains ordinary Tween and phosphate buffer (0.05% Tween-20 in PB).
-
Q.What is the feature of the plate?
ELISA common FAQ -
A.We use plate that is flat bottom and removable strip type plate (8wellx 12 strips).
-
Q.Can I re-use standard after reconstitution?
ELISA common FAQ -
A.Not recommended to re-use standard after reconstitution. Please use it at once after the reconstitution.
Please note that there are some exceptions. One time freeze-thaw the standard is acceptable for use after reconstitution for some ELISAs.
Please check the details on each product datasheet. -
Q.What is different between reagent blank and test sample blank?
ELISA common FAQ -
A.Reagent blank means a well is only added EIA buffer and the purpose is confirming whether the measuring value is influenced by lack of washing process. Test sample blank means a well is added EIA buffer and HRP antibody and the purpose is to calculate the background.
-
Q.How many samples can be measured by this kit?
ELISA common FAQ -
A.The pre-coated plate contained in our ELISA kit is 96 wells plate. We recommend to use 16 wells (2 slits) for standard and 80 wells (10 slits) for 40 samples in duplicate.
-
Q.What is LOD (Limit of Detection)?
ELISA common FAQ -
A.It (LOD) is defined as sensitivity that is calculated using the NCCSL method. Please refer to a datasheet of each product.
-
Q.What is LOQ (Limit of Quantification)?
ELISA common FAQ -
A.It (LOQ) is the lowest value of measurement (standard) range. Please refer to a datasheet of each product.
-
Q.What is the definition of Over Night (O/N) reaction?
ELISA common FAQ -
A.It means that the reaction is required more than 16 hours unless otherwise specifically defined it on a datasheet of each ELISA product.
-
Q.What is the specification of quality control for ELISA product release?
ELISA common FAQ -
A.The information of specification is available on individual lot specific CoA. Please contact us with your reference lot number for obtaining of specific CoA.
-
Q.What is the number (e.g. 432143214321) at the edge of strips of the plate?
ELISA common FAQ -
A.According to the plate maker (ThermoFisher), it does not have any specific meaning as it is just the number of molds.
-
Q.How to wash an ELISA plate?
ELISA common FAQ -
A.Washing it by an auto-washer is highly recommended.
If it is not available, please refer to the demo video (only 2 mins) using a washing bottle.