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#27600 Gd-IgA1 (Galactose-deficient IgA1) Assay Kit - IBL

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  • #27600 Gd-IgA1 (Galactose-deficient IgA1) ELISA Kit
  • Standard curve_#27600 Gd-IgA1 (Galactose-deficient IgA1) ELISA Kit
  • #27600 Gd-IgA1 (Galactose-deficient IgA1) ELISA Kit
  • #27600 Gd-IgA1 (Galactose-deficient IgA1) ELISA Kit
  • #27600 Gd-IgA1 (Galactose-deficient IgA1) ELISA Kit
Intended Use：
Research reagents
Measuring Method：
ELISA
Sample Types：
Human
Measuring Samples：
Serum, EDTA-plasma and Urine
Measurement Range：
1.56 ~ 100 ng/mL
Package Size1：
96 Well

※ The product indicated as "Research reagents" in the column Intended Use cannot be used
　 for diagnostic nor any medical purpose.
※ The datasheet listed on this page is sample only. Please refer to the datasheet
　 enclosed in the product purchased before use.

Product Overview

Product Overview

Product Code 27600
Product Name Gd-IgA1 (Galactose-deficient IgA1) Assay Kit - IBL　
Maker Name Immuno-Biological Laboratories Co., Ltd.
Intended Use Research reagents
Measuring Method ELISA
Conjugate HRP
Species Human
Measuring Samples Serum, EDTA-plasma and Urine
Measurement Range 1.56 ~ 100 ng/mL
Primary Reaction 60 minutes at room temperature
Secondary Reaction 30 minutes at room temperature
Sensitivity 0.488 ng/mL
Storage Condition 2～8°C
Poisonous and Deleterious Substances Not Applicable
Cartagena Not Applicable
Measuring Service Available
Package Size 1 96 Well

Product Description

Product Description

 Galactose-deficient IgA1 (Gd-IgA1) attracts a lot of attentions as a critical effector molecule in the pathogenesis and progression of IgA nephropathy (IgAN) in recent studies. It has been suggested that several O-link glycans modified regions exist in the heavy chain hinge region of human IgA1 molecule andGd-IgA1 circulates in blood stream of the patients with the pathological condition of IgAN. The measuring system using snail (helix aspersa; HAA) lectin that is extracted from snail has been used in past numerous studies and it was revealed that serum levels of Gd-IgA1 in patients with IgAN is significantly elevated compared with the level of healthy subjects or patients with renal diseases other than IgAN. Thus, the importance and purpose of measuring serum Gd-IgA1 level have been gradually recognized from such studies. 

However, since the measuring system used HAA lectin is not suitable for measuring multiple and massive samples in large scale studies due to its instability of glycan-recognizing activity, development of alternative measuring system that can quantitatively measure human Gd-IgA1 in serum with stable and reliable data has been considered as an essential and urgent matter. This IBL ELISA kit using the monoclonal antibody that specifically recognizes galactose-deficient hinge sequence of human Gd-IgA1 is a lectin non-dependent measuring system that can quantitatively measure Gd-IgA1 in human serum,which is also suitable for large scale studies because of its stability.
 

References

References

Note: Retrieve by PMID number in displayed by abstract: http://www.ncbi.nlm.nih.gov

FAQ

FAQ

  • Question
    Q.Does Neuramidase treatment require for the sample?
  • Answer
    A.No it doesn't. There is no much different for the result between with and without Neuramidase.
  • Question
    Q.I found the recommended dilution ratio is between 200 and 800 fold. That is quite a wide range. What is the most optimized dilution ratio?
  • Answer
    A.Some samples have a very high value of Gd-IgA1 and such samples would be range over that why the recommended dilution ratio is wide.
    We recommend to start from 200 fold dilution and find the best dilution ratio for your samples. The optimized dilution ratio should be determined by each user.
  • Question
    Q.What is approx. normal IgA concentration in blood?
  • Answer
    A.Total IgA is approx 1-4mg/mL in blood.
  • Question
    Q.What is approx. normal Gd-IgA1 concentration in blood?
  • Answer
    A.As it is approx 1,000 ng/mL in blood, more than 200 fold dilution is recommended for measurement.
  • Question
    Q.What is the material used for the standard?
  • Answer
    A.It is intact IgA1 (IgA Fab+Fc).
  • Question
    Q.Why the standard is IgA1 instead of Gd-IgA1?
  • Answer
    A.As certain amount of Gd-IgA1 is also contained in normal subject blood samples and the capture antibody(KM55 monoclonal antibody) used in this kit specifically detects Gd-IgA1 so that human IgA1 is applied as the standard.
  • Question
    Q.What is the dilution ratio for urine sample?
  • Answer
    A.Recommended dilution ratio is 2-4 fold using “4 EIA buffer” enclosed in this kit.
  • Question
    Q.How the calibration curve is made?
  • Answer
    A.The calibration curve is made by detecting only Gd-IgA1 from the standard.
  • Question
    Q.How was the STD concentration determined during Assay development?
  • Answer
    A.Please refer to the following paper. In the following paper, Gd-IgA1 is generated from human plasma IgA1.
    Novel lectin-independent approach to detect galactose-deficient IgA1 in IgA nephropathy.
    Yasutake J et al. Nephrol Dial Transplant. 2015 Aug;30(8):1315-21.
    https://pubmed.ncbi.nlm.nih.gov/26109484/
  • Question
    Q.Is control set of this kit available?
  • Answer
    A.Unfortunately, it is not available due to raw material nature.
  • Question
    Q.Is composition of EIA buffer of each ELISA kit all same? Can it be mixed to use?
    ELISA common FAQ
  • Answer
    A.No it isn't. As constitute of each EIA buffer is different, it cannot be mixed with other lots or EIA buffers contained in other kind of ELISA kits.
  • Question
    Q.What is the composition of concentrated wash buffer?
    ELISA common FAQ
  • Answer
    A.It contains ordinary Tween and phosphate buffer (0.05% Tween-20 in PB).
  • Question
    Q.What is the feature of the plate?
    ELISA common FAQ
  • Answer
    A.We use plate that is flat bottom and removable strip type plate (8wellx 12 strips).
  • Question
    Q.Can I re-use standard after reconstitution?
    ELISA common FAQ
  • Answer
    A.Not recommended to re-use standard after reconstitution. Please use it at once after the reconstitution.
    Please note that there are some exceptions. One time freeze-thaw the standard is acceptable for use after reconstitution for some ELISAs.
    Please check the details on each product datasheet.
  • Question
    Q.What is different between reagent blank and test sample blank?
    ELISA common FAQ
  • Answer
    A.Reagent blank means a well is only added EIA buffer and the purpose is confirming whether the Test sample value is influenced by lack of washing process or other operations. Test sample blank means a well is added EIA buffer and HRP antibody and the purpose is to calculate the background.
  • Question
    Q.How many samples can be measured by this kit?
    ELISA common FAQ
  • Answer
    A.The pre-coated plate contained in our ELISA kit is 96 wells plate. We recommend to use 16 wells (2 slits) for standard and 80 wells (10 slits) for 40 samples in duplicate.
  • Question
    Q.What is LOD (Limit of Detection)?
    ELISA common FAQ
  • Answer
    A.It (LOD) is defined as sensitivity that is calculated using the NCCSL method. Please refer to a datasheet of each product.
  • Question
    Q.What is LOQ (Limit of Quantification)?
    ELISA common FAQ
  • Answer
    A.It (LOQ) is the lowest value of measurement (standard) range. Please refer to a datasheet of each product.
  • Question
    Q.What is the definition of Over Night (O/N) reaction?
    ELISA common FAQ
  • Answer
    A.It means that the reaction is required more than 16 hours unless otherwise specifically defined it on a datasheet of each ELISA product.
  • Question
    Q.What is the specification of quality control for ELISA product release?
    ELISA common FAQ
  • Answer
    A.The information of specification is available on individual lot specific CoA. Please contact us with your reference lot number for obtaining of specific CoA.
  • Question
    Q.What is the number (e.g. 432143214321) at the edge of strips of the plate?
    ELISA common FAQ
  • Answer
    A.According to the plate maker (ThermoFisher), it does not have any specific meaning as it is just the number of molds.
  • Question
    Q.How to wash an ELISA plate?
    ELISA common FAQ
  • Answer
    A.Washing it by an auto-washer is highly recommended.
    If it is not available, please refer to the demo video (only 2 mins) using a washing bottle.
  • Question
    Q.The wells turned black during the test with the kit.
    ELISA common FAQ
  • Answer
    A.It is possible that the wells were not washed sufficiently during the washing process after the HRP-labeled antibody reaction.
    Be sure to wash the wells enough times as described in the data sheet with washing buffer of more than 350 µL.

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