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#27102 Mouse VEGF Assay Kit - IBL

  • #27102 Mouse VEGF ELISA Kit
  • #27102 Mouse VEGF ELISA Kit
Intended Use:
Research reagents
Measuring Method:
ELISA
Sample Types:
Mouse
Measuring Samples:
EDTA-plasma, Serum, Cell culture supernatant
Measurement Range:
62.5 ~ 4,000 pg/mL
Package Size1:
96 Well

※ The product indicated as "Research reagents" in the column Intended Use cannot be used
  for diagnostic nor any medical purpose.
※ The datasheet listed on this page is sample only. Please refer to the datasheet
  enclosed in the product purchased before use.

Product Overview

Product Overview

Product Code 27102
Product Name Mouse VEGF Assay Kit - IBL
Intended Use Research reagents
Measuring Method ELISA
Conjugate HRP
Species Mouse
Measuring Samples EDTA-plasma, Serum, Cell culture supernatant
Measurement Range 62.5 ~ 4,000 pg/mL
Primary Reaction 60 minutes at 37 ℃
Secondary Reaction 30 minutes at 2 - 8 ℃
Sensitivity 10.98 pg/mL
Specificity Compound Cross Reactivity
Mouse VEGF 164 100.0%
Human VEGF 165 ≦0.1 %
Storage Condition 2 - 8 ℃
Poisonous and Deleterious Substances Not Applicable
Cartagena Not Applicable
Measuring Service Not Available
Package Size 1 96 Well

Product Description

Product Description

Vascular Endothelial Cell Growth Factor (VEGF) is a homodimeric protein initially purified from media conditioned by normal bovine pituitary folliculo-stellate cells and secreted by a variety of vascularized tissues. It was subsequently found to be identical to a vascular permeability factor (VPF), which was previously identified in media conditioned by tumor cell lines based upon its ability to increase the permeability of capillary blood vessels. The reported activities of VEGF include stimulation of endothelial cell growth, angiogenesis and capillary permeability. Human VEGF is a 38.2kDa homodimeric protein consisting of two 165 amino acid polypeptide chains. VEGF is expressed in many human tumor cells, including human adenocarcinoma, human pancreatic carcinoma, human hepatocellular carcinoma, renal cell carcinoma, fibrosarcoma, HL60 promyelocytic leukemia, GS-9L glioma and U937 lymphoma cells. In normal tissues, VEGF expression has been observed in activated macrophages, keratinocytes, hepatocytes, smooth muscle cells Leydig cells, embryonic fibroblasts and bronchial and choroids plexus epithelium, renal glomerular visceral epithelium and mesangial cells. In contrast to VEGF, VEGF-C, which was discovered as a VEGF-associated molecule, has been identified as a molecule that stimulates lymph vessel formation through VEGFR-3, its specific lymphatic endothelium receptor. The existence of human VEGF-C molecules having different molecular weights as a result of proteolytic processing has been reported. The kit is a complete kit for the quantitative determination of mouse VEGF.

References

References

Note: Retrieve by PMID number in displayed by abstract: http://www.ncbi.nlm.nih.gov

FAQ

FAQ

  • Question
    Q.Does this assay kit detect any specific type of VEGF or any type of VEGF such as A, B, C and D?
  • Answer
    A.It cross reacts with VEGF-A. Isoforms like 120, 164 and 188 can be measured as mixed substances if the sample is lysate.
  • Question
    Q.Is it possible to measure VEGF expressed in lysate of mice muscle?
  • Answer
    A.If it is an ordinary tissue lysate, it can be measured by the assay kit.
  • Question
    Q.Is composition of EIA buffer of each ELISA kit all same? Can it be mixed to use?
    ELISA common FAQ
  • Answer
    A.No it isn't. As constitute of each EIA buffer is different, it cannot be mixed with other lots or EIA buffers contained in other kind of ELISA kits.
  • Question
    Q.What is the composition of concentrated wash buffer?
    ELISA common FAQ
  • Answer
    A.It contains ordinary Tween and phosphate buffer (0.05% Tween-20 in PB).
  • Question
    Q.What is the feature of the plate?
    ELISA common FAQ
  • Answer
    A.We use plate that is flat bottom and removable strip type plate (8wellx 12 strips).
  • Question
    Q.Can I re-use standard after reconstitution?
    ELISA common FAQ
  • Answer
    A.Not recommended to re-use standard after reconstitution. Please use it at once after the reconstitution.
    Please note that there are some exceptions. One time freeze-thaw the standard is acceptable for use after reconstitution for some ELISAs.
    Please check the details on each product datasheet.
  • Question
    Q.What is different between reagent blank and test sample blank?
    ELISA common FAQ
  • Answer
    A.Reagent blank means a well is only added EIA buffer and the purpose is confirming whether the measuring value is influenced by lack of washing process. Test sample blank means a well is added EIA buffer and HRP antibody and the purpose is to calculate the background.
  • Question
    Q.How many samples can be measured by this kit?
    ELISA common FAQ
  • Answer
    A.The pre-coated plate contained in our ELISA kit is 96 wells plate. We recommend to use 16 wells (2 slits) for standard and 80 wells (10 slits) for 40 samples in duplicate.
  • Question
    Q.What is LOD (Limit of Detection)?
    ELISA common FAQ
  • Answer
    A.It (LOD) is defined as sensitivity that is calculated using the NCCSL method. Please refer to a datasheet of each product.
  • Question
    Q.What is LOQ (Limit of Quantification)?
    ELISA common FAQ
  • Answer
    A.It (LOQ) is the lowest value of measurement (standard) range. Please refer to a datasheet of each product.

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