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#27171 Human VEGF Assay Kit - IBL
- Intended Use:
- Research reagents
- Measuring Method:
- ELISA
- Sample Types:
- Human
- Measuring Samples:
- EDTA-plasma,Cell culture supernatant
- Measurement Range:
- 15.63 ~ 1,000 pg/mL (1st.Incubation for 60 minutes at 37℃)
7.81 ~ 500 pg/mL (1st. Incubation overnight at 4℃)
- Package Size1:
- 96 Well
※ The product indicated as "Research reagents" in the column Intended Use cannot be used
for diagnostic nor any medical purpose.
※ The datasheet listed on this page is sample only. Please refer to the datasheet
enclosed in the product purchased before use.
Product Overview
Product Overview
Product Code | 27171 |
---|---|
Product Name | Human VEGF Assay Kit - IBL |
Intended Use | Research reagents |
Measuring Method | ELISA |
Conjugate | HRP |
Species | Human |
Measuring Samples | EDTA-plasma,Cell culture supernatant |
Measurement Range | 15.63 ~ 1,000 pg/mL (1st.Incubation for 60 minutes at 37℃) 7.81 ~ 500 pg/mL (1st. Incubation overnight at 4℃) |
Primary Reaction | 60 minutes at 37℃ or Overnight at 2 - 8℃ |
Secondary Reaction | 30 minutes at 2 - 8℃ |
Sensitivity | 1.01 pg/mL |
Specificity | Compound Cross Reactivity Human VEGF 165 100.0% Human VEGF 121 39.7% Human PDGF < 0.1% |
Storage Condition | 2 - 8 ℃ |
Poisonous and Deleterious Substances | Not Applicable |
Cartagena | Not Applicable |
Measuring Service | Not Available |
Package Size 1 | 96 Well |
Product Description
Product Description
Vascular Endothelial Cell Growth Factor (VEGF) is a homodimeric protein initially purified from media conditioned by normal bovine pituitary folliculo-stellate cells and secreted by a variety of vascularized tissues. It was subsequently found to be identical to a vascular permeability factor (VPF), which was previously identified in media conditioned by tumor cell lines based upon its ability to increase the permeability of capillary blood vessels. The reported activities of VEGF include stimulation of endothelial cell growth, angiogenesis and capillary permeability. Human VEGF is a 38.2kDa homodimeric protein consisting of two 165 amino acid polypeptide chains. VEGF is expressed in many human tumor cells, including human adenocarcinoma, human pancreatic carcinoma, human hepatocellular carcinoma, renal cell carcinoma, fibrosarcoma, HL60 promyelocytic leukemia, GS-9L glioma and U937 lymphoma cells. In normal tissues, VEGF expression has been observed in activated macrophages, keratinocytes, hepatocytes, smooth muscle cells Leydig cells, embryonic fibroblasts and bronchial and choroids plexus epithelium, renal glomerular visceral epithelium and mesangial cells. In contrast to VEGF, VEGF-C, which was discovered as a VEGF-associated molecule, has been identified as a molecule that stimulates lymph vessel formation through VEGFR-3, its specific lymphatic endothelium receptor. The existence of human VEGF-C molecules having different molecular weights as a result of proteolytic processing has been reported.
References
References
- Serum and peritoneal fluid levels of vascular endothelial growth factor in women with endometriosis. Kianpour M et.al. Int J Fertil Steril. 2013 Jul;7(2):96-9. Epub 2013 Jul 31.PMID: 24520470
- Clinical implications of expression of cyclooxygenase-2 related to angiogenesis in uterine endometrial cancers Toyoki H et al. Ann Oncol. 2005 Jan;16(1):51-5.PMID: 15598938
- Expression of cyclooxygenase-2 related to angiogenesis in uterine cervical
cancers Fujimoto J et al. J Biomed Sci. 2006 Nov;13(6):825-32.PMID: 17013750 - Clinical implications of expression of cyclooxygenase-2 related to angiogenesis in ovarian cancer. Fujimoto J et al. Oncol Rep. 2006 Jan;15(1):21-5.PMID: 16328030
- COX-2-mediated stimulation of the lymphangiogenic factor VEGF-C in human breast cancer A V Timoshenko et al. Br J Cancer. 2006 Apr 24; 94(8): 1154-1163.PMID: 16570043
- Activation of hepatocyte growth factor/scatter factor in colorectal carcinoma. Kataoka H et al. Cancer Res. 2000 Nov 1;60(21):6148-59.PMID: 11085539
- Cyclooxygenase-2-regulated vascular endothelial growth factor release in gastric fibroblasts. Miura S et al. Am J Physiol Gastrointest Liver Physiol. 2004 Aug;287(2):G444-51.PMID: 15246970
- Biologic implications of the expression of vascular endothelial growth factor subtypes in ovarian carcinoma. Fujimoto J et al. Cancer. 1998 Dec 15;83(12):2528-33.PMID: 9874459
- Multi-kinase inhibitor E7080 suppresses lymph node and lung metastases of human mammary breast tumor MDA-MB-231 via inhibition of vascular endothelial growth factor-receptor (VEGF-R) 2 and VEGF-R3 kinase. Matsui J et al. Clin Cancer Res. 2008 Sep 1;14(17):5459-65.PMID: 18765537
- Induction of vasculogenesis in breast cancer models. Shirakawa K et al. Br J Cancer. 2002 Dec 2;87(12):1454-61.PMID: 12454777
Note: Retrieve by PMID number in displayed by abstract: http://www.ncbi.nlm.nih.gov
FAQ
FAQ
-
Q.Is there any cross reactivity other than VEGF161?
-
A.The specificity is described in “10. Kit performance” on the datasheet. Please refer to the datasheet for additional information.
-
Q.Can this kit be used for plasma samples?
-
A.EDTA-plasma can be used. However, heparin-plasma cannot be used for the assay.
-
Q.What is recombinant VEGF derived from?
-
A.It is a supernatant of CHO.
-
Q.Can this kit be used for mice and rat samples?
-
A.No, it cannot be used for those animal samples.
-
Q.Is composition of EIA buffer of each ELISA kit all same? Can it be mixed to use?
ELISA common FAQ -
A.No it isn't. As constitute of each EIA buffer is different, it cannot be mixed with other lots or EIA buffers contained in other kind of ELISA kits.
-
Q.What is the composition of concentrated wash buffer?
ELISA common FAQ -
A.It contains ordinary Tween and phosphate buffer (0.05% Tween-20 in PB).
-
Q.What is the feature of the plate?
ELISA common FAQ -
A.We use plate that is flat bottom and removable strip type plate (8wellx 12 strips).
-
Q.Can I re-use standard after reconstitution?
ELISA common FAQ -
A.Not recommended to re-use standard after reconstitution. Please use it at once after the reconstitution.
Please note that there are some exceptions. One time freeze-thaw the standard is acceptable for use after reconstitution for some ELISAs.
Please check the details on each product datasheet. -
Q.What is different between reagent blank and test sample blank?
ELISA common FAQ -
A.Reagent blank means a well is only added EIA buffer and the purpose is confirming whether the Test sample value is influenced by lack of washing process or other operations. Test sample blank means a well is added EIA buffer and HRP antibody and the purpose is to calculate the background.
-
Q.How many samples can be measured by this kit?
ELISA common FAQ -
A.The pre-coated plate contained in our ELISA kit is 96 wells plate. We recommend to use 16 wells (2 slits) for standard and 80 wells (10 slits) for 40 samples in duplicate.
-
Q.What is LOD (Limit of Detection)?
ELISA common FAQ -
A.It (LOD) is defined as sensitivity that is calculated using the NCCSL method. Please refer to a datasheet of each product.
-
Q.What is LOQ (Limit of Quantification)?
ELISA common FAQ -
A.It (LOQ) is the lowest value of measurement (standard) range. Please refer to a datasheet of each product.
-
Q.What is the definition of Over Night (O/N) reaction?
ELISA common FAQ -
A.It means that the reaction is required more than 16 hours unless otherwise specifically defined it on a datasheet of each ELISA product.
-
Q.What is the specification of quality control for ELISA product release?
ELISA common FAQ -
A.The information of specification is available on individual lot specific CoA. Please contact us with your reference lot number for obtaining of specific CoA.
-
Q.What is the number (e.g. 432143214321) at the edge of strips of the plate?
ELISA common FAQ -
A.According to the plate maker (ThermoFisher), it does not have any specific meaning as it is just the number of molds.
-
Q.How to wash an ELISA plate?
ELISA common FAQ -
A.Washing it by an auto-washer is highly recommended.
If it is not available, please refer to the demo video (only 2 mins) using a washing bottle. -
Q.The wells turned black during the test with the kit.
ELISA common FAQ -
A.It is possible that the wells were not washed sufficiently during the washing process after the HRP-labeled antibody reaction.
Be sure to wash the wells enough times as described in the data sheet with washing buffer of more than 350 µL.