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#27755 Human Galectin-3 Assay Kit - IBL

Intended Use:
Research reagents
Measuring Method:
ELISA
Sample Types:
Human
Measuring Samples:
EDTA-plasma, Serum, Cell extract, Tissue extract, Cell culture supernatant
Measurement Range:
117.19 ~ 7,500 pg/mL
Package Size1:
96 Well

※ The product indicated as "Research reagents" in the column Intended Use cannot be used
  for diagnostic nor any medical purpose.
※ The datasheet listed on this page is sample only. Please refer to the datasheet
  enclosed in the product purchased before use.

Product Overview

Product Overview

Product Code 27755
Product Name Human Galectin-3 Assay Kit - IBL
Intended Use Research reagents
Measuring Method ELISA
Conjugate HRP
Species Human
Measuring Samples EDTA-plasma, Serum, Cell extract, Tissue extract, Cell culture supernatant
Measurement Range 117.19 ~ 7,500 pg/mL
Primary Reaction 60minutes at 37 ℃
Secondary Reaction 30 minutes at 2 - 8 ℃
Sensitivity 43.95 pg/mL
Specificity Compound Cross Reactivity
Human Galectin-3 100.0%
Human Galectin-1 ≦0.1%
Human Galectin-4 0.21%
Mouse Galectin-3 ≦0.1%
Storage Condition 2 - 8 ℃
Poisonous and Deleterious Substances Not Applicable
Cartagena Not Applicable
Measuring Service Not Available
Package Size 1 96 Well

Product Description

Product Description

Galectin is widely distributed in nematodes, insects, and porifers, as well as vertebrates, and it has also been found to be present in true fungi. Galectin does not just occur in the cytoplasm, it is also present in the nucleus, on the cell surface, in the extracellular matrix, etc., and it is thought to be involved in many biological phenomena, including development, differentiation, morphogenesis, tumor metastasis, cell death, and RNA splicing. Galectin-3 is a β-galactoside-binding protein that has been named IgE-binding protein, CBP35, CBP30, Mac-2, L-29, L-31, L-34, etc., and structurally it is a chimera-type lectin composed of a sugar-chain-binding domain (galectin domain) and a non-lectin domain. Its biological function is still uncertain, but many studies that should elucidate its function have been performed, and as a result participation of galectin-3 has been demonstrated in the biological phenomena of cell growth, adhesion, metastasis, and apoptosis. For example, a positive correlation has been shown between galectin-3 expression and the degree of malignant transformation in certain types of cell lines. A positive correlation has also recently been shown between galectin-3 expression and degree of malignancy in certain types of malignant tumors, and measurement of galectin-3 is expected to possibly serve as an index of degree of tumor malignancy.

FAQ

FAQ

  • Question
    Q.What is the epitope of the pre-coated antibody (anti-human Gal-3 (50A3) mouse IgG MoAb)?
  • Answer
    A.The epitope of 50A3 is the 64 amino acid residue of the N-terminal region of Galectin-3.
  • Question
    Q.Does this antibody (the anti-human Gal-3 (50A3)) only bind to the 64th a.a. residue of Galectin-3?
  • Answer
    A.The antibody reacts to a.a. parts 1 to 64, which means the antibody can detect any part of Galectin-3 found in this portion (1 to 64). Thus, the antibody can also detect the full length of Galectin-3.
  • Question
    Q.Is composition of EIA buffer of each ELISA kit all same? Can it be mixed to use?
    ELISA common FAQ
  • Answer
    A.No it isn't. As constitute of each EIA buffer is different, it cannot be mixed with other lots or EIA buffers contained in other kind of ELISA kits.
  • Question
    Q.What is the composition of concentrated wash buffer?
    ELISA common FAQ
  • Answer
    A.It contains ordinary Tween and phosphate buffer (0.05% Tween-20 in PB).
  • Question
    Q.What is the feature of the plate?
    ELISA common FAQ
  • Answer
    A.We use plate that is flat bottom and removable strip type plate (8wellx 12 strips).
  • Question
    Q.Can I re-use standard after reconstitution?
    ELISA common FAQ
  • Answer
    A.Not recommended to re-use standard after reconstitution. Please use it at once after the reconstitution.
    Please note that there are some exceptions. One time freeze-thaw the standard is acceptable for use after reconstitution for some ELISAs.
    Please check the details on each product datasheet.
  • Question
    Q.What is different between reagent blank and test sample blank?
    ELISA common FAQ
  • Answer
    A.Reagent blank means a well is only added EIA buffer and the purpose is confirming whether the measuring value is influenced by lack of washing process. Test sample blank means a well is added EIA buffer and HRP antibody and the purpose is to calculate the background.
  • Question
    Q.How many samples can be measured by this kit?
    ELISA common FAQ
  • Answer
    A.The pre-coated plate contained in our ELISA kit is 96 wells plate. We recommend to use 16 wells (2 slits) for standard and 80 wells (10 slits) for 40 samples in duplicate.
  • Question
    Q.What is LOD (Limit of Detection)?
    ELISA common FAQ
  • Answer
    A.It (LOD) is defined as sensitivity that is calculated using the NCCSL method. Please refer to a datasheet of each product.
  • Question
    Q.What is LOQ (Limit of Quantification)?
    ELISA common FAQ
  • Answer
    A.It (LOQ) is the lowest value of measurement (standard) range. Please refer to a datasheet of each product.