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#27762 α2, 6-Sialyltransferase Assay Kit - IBL

  • 27762 α2, 6-Sialyltransferase ELISA Kit
Intended Use:
Research reagents
Measuring Method:
ELISA
Sample Types:
Human, Mouse, Rat
Measuring Samples:
EDTA-plasma, Serum, Cell culture supernatant
Measurement Range:
1.09 ~ 70 ng/mL
Package Size1:
96 Well

※ The product indicated as "Research reagents" in the column Intended Use cannot be used
  for diagnostic nor any medical purpose.
※ The datasheet listed on this page is sample only. Please refer to the datasheet
  enclosed in the product purchased before use.

Product Overview

Product Code 27762
Product Name α2, 6-Sialyltransferase Assay Kit - IBL
Intended Use Research reagents
Measuring Method ELISA
Conjugate HRP
Species Human, Mouse, Rat
Measuring Samples EDTA-plasma, Serum, Cell culture supernatant
Measurement Range 1.09 ~ 70 ng/mL
Primary Reaction 60 minutes at 37 ℃
Secondary Reaction 30 minutes at 2 - 8 ℃
Sensitivity 0.20 ng/mL
Storage Condition 2 - 8 ℃
Poisonous and Deleterious Substances Not Applicable
Cartagena Not Applicable
Measuring Service Not Available
Package Size 1 96 Well

Product Description

The histopathological picture of Alzheimer’s disease is characterized by senile plaques and neurofibrillary tangles, and because the senile plaques form first, they are considered the initial lesion. Senile plaques are known to be formed by accumulation of β-amyloid peptide (Aβ). Aβ peptide is produced by the cleavage of amyloid precursor protein (APP) by two types of proteolytic enzymes. The first cleavage is performed by β-secretase (BACE1) and the secondγ-secretase. In recent years a glycosyltransferase involved in the biosynthesis of sugar chains (α2, 6-sialyltransferase) has also been shown to be cleaved by BACE1. The cleavage site was identified at the same time, and as a result it was demonstrated that in rats it produces cleavaged-type α2, 6-sialyltransferase (E41 Form). This product is a measurement kit that enables detection of human, rat and mouse α2, 6-sialyltransferase.

The control set is available for sale on request. Learn More

FAQ

  • Question
    Q.Is composition of EIA buffer of each ELISA kit all same? Can it be mixed to use?
    ELISA common FAQ
  • Answer
    A.No it isn't. As constitute of each EIA buffer is different, it cannot be mixed with other lots or EIA buffers contained in other kind of ELISA kits.
  • Question
    Q.What is the composition of concentrated wash buffer?
    ELISA common FAQ
  • Answer
    A.It contains ordinary Tween and phosphate buffer (0.05% Tween-20 in PB).
  • Question
    Q.What is the feature of the plate?
    ELISA common FAQ
  • Answer
    A.We use plate that is flat bottom and removable strip type plate (8wellx 12 strips).
  • Question
    Q.Can I re-use standard after reconstitution?
    ELISA common FAQ
  • Answer
    A.Not recommended to re-use standard after reconstitution. Please use it at once after the reconstitution.
    Please note that there are some exceptions. One time freeze-thaw the standard is acceptable for use after reconstitution for some ELISAs.
    Please check the details on each product datasheet.
  • Question
    Q.What is different between reagent blank and test sample blank?
    ELISA common FAQ
  • Answer
    A.Reagent blank means a well is only added EIA buffer and the purpose is confirming whether the measuring value is influenced by lack of washing process. Test sample blank means a well is added EIA buffer and HRP antibody and the purpose is to calculate the background.
  • Question
    Q.How many samples can be measured by this kit?
    ELISA common FAQ
  • Answer
    A.The pre-coated plate contained in our ELISA kit is 96 wells plate. We recommend to use 16 wells (2 slits) for standard and 80 wells (10 slits) for 40 samples in duplicate.
  • Question
    Q.What is LOD (Limit of Detection)?
    ELISA common FAQ
  • Answer
    A.It (LOD) is defined as sensitivity that is calculated using the NCCSL method. Please refer to a datasheet of each product.
  • Question
    Q.What is LOQ (Limit of Quantification)?
    ELISA common FAQ
  • Answer
    A.It (LOQ) is the lowest value of measurement (standard) range. Please refer to a datasheet of each product.

Note: This product is for research use only. It cannot be used as a diagnostic tool or drug.