Product Search

#27769 Human LRG Assay Kit - IBL (Discontinued : until the raw materials are ended)

  • Hepatitis (PBC) Research Data
Intended Use：
Research reagents
Measuring Method：
ELISA
Sample Types：
Human
Measuring Samples：
EDTA-plasma, Serum, Urine, CSF (※ Additional EIA buffer may be required for blood samples. The EIA buffer is available with charge. Please refer to the datasheet for the details.)
Measurement Range：
1.56 ～ 100 ng/mL
Package Size1：
96 Well

※ The product indicated as "Research reagents" in the column Intended Use cannot be used
　 for diagnostic nor any medical purpose.
※ The datasheet listed on this page is sample only. Please refer to the datasheet
　 enclosed in the product purchased before use.

Product Overview

Product Overview

Product Code 27769
Product Name Human LRG Assay Kit - IBL (Discontinued : until the raw materials are ended)
Intended Use Research reagents
Measuring Method ELISA
Conjugate HRP
Species Human
Measuring Samples EDTA-plasma, Serum, Urine, CSF (※ Additional EIA buffer may be required for blood samples. The EIA buffer is available with charge. Please refer to the datasheet for the details.)
Measurement Range 1.56 ～ 100 ng/mL
Primary Reaction 18 hours at 4 °C
Secondary Reaction 30 minutes at 37 ℃
Sensitivity 0.17 ng/mL
Specificity Compound Cross Reactivity
Human LRG 100.0%
Human Aβ (1-42) < 0.01%
Human sAPPβ (w) < 0.01%
Human sAPPα < 0.01%
Storage Condition 2 - 8 ℃
Poisonous and Deleterious Substances Not Applicable
Cartagena Not Applicable
Measuring Service Not Available
Package Size 1 96 Well
Remarks1 ※ Additional EIA buffer may be required for blood samples. The EIA buffer is available with charge. Please refer to the datasheet for the details.

The following product is available with charge if an additional buffer is required. Please note, this buffer can be only used for #27769. It cannot be used for Mouse or Rat LRG Assay Kits.

Product Code: 27769D100
Product Name: Human LRG EIA buffer 100
Size: 100 mL

Product Description

Product Description

Among the diseases which should be distinguished from iNPH (idiopathic normal pressure hydrocephalus) of elderly people showing symptoms such as gait disorder and dementia, there are some neurodegenerative diseases such as Alzheimer's disease (AD), FTLD (frontotemporal lobar degeneration) and disorder associated with Parkinson's disease. This ELISA kit can measure LRG in CSF, blood or urine. It has become possible to distinguish iNPH from neurodegenerative diseases such as AD by measuring LRG in CSF.


#27769-96Well Human LRG Assay Kit – IBL will be replaced to the new kit for effective and stable supply.

(New)
#27798-96Well Human LRG(Mono) ELISA Kit - IBL
#27798D100-100mL Human LRG(Mono) EIA buffer 100

Supplying of 27769-96Well Human LRG Assay Kit – IBL and its buffer (#27769D100-100mL Human LRG EIA buffer 100) until the raw materials are ended.

【Major changed from 27769 (current) to 27798 (new) 】
1. Use monoclonal paired antibodies instead of polyclonal one.
2. Only blood samples are available for measurement (Not CSF samples anymore).

References

References

Note: Retrieve by PMID number in displayed by abstract: http://www.ncbi.nlm.nih.gov

FAQ

FAQ

  • Question
    Q.What is the expression system of the standard?
  • Answer
    A.It is a recombinant protein expression system. Wheat germ is used in this system.
  • Question
    Q.What is the recommended dilution ratio?
  • Answer
    A.We cannot recommend a specific dilution ratio, but we generally use a dilution magnification of anywhere between 2 to 16 when we conduct measurements.
  • Question
    Q.What is the epitopes of the pre-coated antibodies used in this kit?
  • Answer
    A.The epitope of the anntibody (Human LRG (162) Rabbit IgG A.P.) is GLKALGHLSGNRLRKL.
  • Question
    Q.What is the epitope of the labeled antibody?
  • Answer
    A.The epitope of the labeled antibody (Human LRG (329) Rabbit IgG Fab’ A.P.) is AGPEAVKGQTLLAVAKSQ.
  • Question
    Q.Is there any data available for the measurement example and the reproducibility using normal controls?
  • Answer
    A.Yes there is. The date is available on the following relevant product page. Please refer to the Pamphlet 2 listed on the page.
    http://www.ibl-japan.co.jp/en/search/product/detail/id=3945
  • Question
    Q.Does this kit specifically detect LRG1 or other LRG families as well?
  • Answer
    A.We have not actually checked that the cross reactivity against other LRG families using our LRG-1 kit #27769, however, as epitope of the antibodies that are used for ELISA kit are different positions from Leucine-rich repeat region, theoretically we believe that it specifically detects LRG1 and does not cross react to other LRG families.
  • Question
    Q.Is composition of EIA buffer of each ELISA kit all same? Can it be mixed to use?
    ELISA common FAQ
  • Answer
    A.No it isn't. As constitute of each EIA buffer is different, it cannot be mixed with other lots or EIA buffers contained in other kind of ELISA kits.
  • Question
    Q.What is the composition of concentrated wash buffer?
    ELISA common FAQ
  • Answer
    A.It contains ordinary Tween and phosphate buffer (0.05% Tween-20 in PB).
  • Question
    Q.What is the feature of the plate?
    ELISA common FAQ
  • Answer
    A.We use plate that is flat bottom and removable strip type plate (8wellx 12 strips).
  • Question
    Q.Can I re-use standard after reconstitution?
    ELISA common FAQ
  • Answer
    A.Not recommended to re-use standard after reconstitution. Please use it at once after the reconstitution.
    Please note that there are some exceptions. One time freeze-thaw the standard is acceptable for use after reconstitution for some ELISAs.
    Please check the details on each product datasheet.
  • Question
    Q.What is different between reagent blank and test sample blank?
    ELISA common FAQ
  • Answer
    A.Reagent blank means a well is only added EIA buffer and the purpose is confirming whether the Test sample value is influenced by lack of washing process or other operations. Test sample blank means a well is added EIA buffer and HRP antibody and the purpose is to calculate the background.
  • Question
    Q.How many samples can be measured by this kit?
    ELISA common FAQ
  • Answer
    A.The pre-coated plate contained in our ELISA kit is 96 wells plate. We recommend to use 16 wells (2 slits) for standard and 80 wells (10 slits) for 40 samples in duplicate.
  • Question
    Q.What is LOD (Limit of Detection)?
    ELISA common FAQ
  • Answer
    A.It (LOD) is defined as sensitivity that is calculated using the NCCSL method. Please refer to a datasheet of each product.
  • Question
    Q.What is LOQ (Limit of Quantification)?
    ELISA common FAQ
  • Answer
    A.It (LOQ) is the lowest value of measurement (standard) range. Please refer to a datasheet of each product.
  • Question
    Q.What is the definition of Over Night (O/N) reaction?
    ELISA common FAQ
  • Answer
    A.It means that the reaction is required more than 16 hours unless otherwise specifically defined it on a datasheet of each ELISA product.
  • Question
    Q.What is the specification of quality control for ELISA product release?
    ELISA common FAQ
  • Answer
    A.The information of specification is available on individual lot specific CoA. Please contact us with your reference lot number for obtaining of specific CoA.
  • Question
    Q.What is the number (e.g. 432143214321) at the edge of strips of the plate?
    ELISA common FAQ
  • Answer
    A.According to the plate maker (ThermoFisher), it does not have any specific meaning as it is just the number of molds.
  • Question
    Q.How to wash an ELISA plate?
    ELISA common FAQ
  • Answer
    A.Washing it by an auto-washer is highly recommended.
    If it is not available, please refer to the demo video (only 2 mins) using a washing bottle.
  • Question
    Q.The wells turned black during the test with the kit.
    ELISA common FAQ
  • Answer
    A.It is possible that the wells were not washed sufficiently during the washing process after the HRP-labeled antibody reaction.
    Be sure to wash the wells enough times as described in the data sheet with washing buffer of more than 350 µL.
Contact Us >