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#27996 Human FGF19 Assay Kit - IBL

  • 27996 Human FGF19 ELISA Kit
  • 27996 Human FGF19 ELISA Kit
  • 27996 Human FGF19 ELISA Kit
  • 27996 Human FGF19 ELISA Kit
  • 27996 Human FGF19 ELISA Kit
  • 27996 Human FGF19 ELISA Kit
Intended Use:
Research reagents
Measuring Method:
ELISA
Sample Types:
Human
Measuring Samples:
EDTA-plasma, Cell culture supernatant
Measurement Range:
23.4 ~ 1,500 pg/mL
Package Size1:
96 Well

※ The product indicated as "Research reagents" in the column Intended Use cannot be used
  for diagnostic nor any medical purpose.
※ The datasheet listed on this page is sample only. Please refer to the datasheet
  enclosed in the product purchased before use.

Product Overview

Product Overview

Product Code 27996
Product Name Human FGF19 Assay Kit - IBL
Intended Use Research reagents
Measuring Method ELISA
Conjugate HRP
Species Human
Measuring Samples EDTA-plasma, Cell culture supernatant
Measurement Range 23.4 ~ 1,500 pg/mL
Primary Reaction Overnight at 2 - 8 ℃
Secondary Reaction 30 minutes at 2 - 8 ℃
Sensitivity 7.2 pg/mL
Specificity Substance Cross-Reactivity
Human FGF19 100.0%
Human FGF21 <0.1 %
Storage Condition 2 - 8 ℃
Poisonous and Deleterious Substances Not Applicable
Cartagena Not Applicable
Measuring Service Not Available
Package Size 1 96 Well

Product Description

Product Description

FGF19 subfamily composed of FGF19, FGF21 and FGF23 binds the receptors, α/βKlotho and it is characterized as a factor group which has hormonal functions. The novel system consisted of α/βKlotho and FGF19 subfamily regulates integrally metabolism of minerals, lipids, energy and amino acids. This novel regulating system involves important homeostatic mechanism for biological body such as metabolism of bile acid and cholesterol in the liver (FGF19), energy and glycolipids metabolism (FGF21) and phosphate, calcium and vitamin D metabolism in kidney (FGF23) in fasting state. FGF19 is secreted from intestine and transported to liver through bloodstream. It suppresses synthesis of bile acid in liver and regulates the function of liver that preserves sugar as a glycogen. FGF19 binds a receptor consisted of FGFR4 and βKlotho. FGF19 is studied in relation to digestive disorders such as diarrhea and constipation which might be related to FGF19 hormonal abnormality and metabolic disorders such as diabetes. The concentration of FGF19 in blood is measured and reported its involvement with many disease such as polycystic ovary syndrome history, bile acid diarrhea, coronary artery disease, pediatric nonalcoholic fatty liver disease, impaired fasting glucose, quiescent Crohn's colitis, hepatocellular carcinoma, type 2 diabetic patients with metabolic syndrome and anorexia nervosa. This kit can assay Human blood FGF19 density.

The control set is available for sale on request. Learn More

FAQ

FAQ

  • Question
    Q.Can serum samples be used for this kit?
  • Answer
    A.No, it cannot.
  • Question
    Q.Is composition of EIA buffer of each ELISA kit all same? Can it be mixed to use?
    ELISA common FAQ
  • Answer
    A.No it isn't. As constitute of each EIA buffer is different, it cannot be mixed with other lots or EIA buffers contained in other kind of ELISA kits.
  • Question
    Q.What is the composition of concentrated wash buffer?
    ELISA common FAQ
  • Answer
    A.It contains ordinary Tween and phosphate buffer (0.05% Tween-20 in PB).
  • Question
    Q.What is the feature of the plate?
    ELISA common FAQ
  • Answer
    A.We use plate that is flat bottom and removable strip type plate (8wellx 12 strips).
  • Question
    Q.Can I re-use standard after reconstitution?
    ELISA common FAQ
  • Answer
    A.Not recommended to re-use standard after reconstitution. Please use it at once after the reconstitution.
    Please note that there are some exceptions. One time freeze-thaw the standard is acceptable for use after reconstitution for some ELISAs.
    Please check the details on each product datasheet.
  • Question
    Q.What is different between reagent blank and test sample blank?
    ELISA common FAQ
  • Answer
    A.Reagent blank means a well is only added EIA buffer and the purpose is confirming whether the measuring value is influenced by lack of washing process. Test sample blank means a well is added EIA buffer and HRP antibody and the purpose is to calculate the background.
  • Question
    Q.How many samples can be measured by this kit?
    ELISA common FAQ
  • Answer
    A.The pre-coated plate contained in our ELISA kit is 96 wells plate. We recommend to use 16 wells (2 slits) for standard and 80 wells (10 slits) for 40 samples in duplicate.
  • Question
    Q.What is LOD (Limit of Detection)?
    ELISA common FAQ
  • Answer
    A.It (LOD) is defined as sensitivity that is calculated using the NCCSL method. Please refer to a datasheet of each product.
  • Question
    Q.What is LOQ (Limit of Quantification)?
    ELISA common FAQ
  • Answer
    A.It (LOQ) is the lowest value of measurement (standard) range. Please refer to a datasheet of each product.
  • Question
    Q.What is the definition of Over Night (O/N) reaction?
  • Answer
    A.It means that the reaction is required more than 16 hours unless otherwise specifically defined it on a datasheet of each ELISA product.

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