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#27110 Human CD134/OX40 Assay Kit - IBL

  • #27110 Human CD134/OX40 ELISA Kit
  • #27110 Human CD134/OX40 ELISA Kit
  • #27110 Human CD134/OX40 ELISA Kit
  • #27110 Human CD134/OX40 ELISA Kit
  • #27110 Human CD134/OX40 ELISA Kit
Intended Use:
Research reagents
Measuring Method:
ELISA
Sample Types:
Human
Measuring Samples:
Serum, EDTA-plasma, Cell culture supernatant
Measurement Range:
15.6 - 1,000 pg/mL
Package Size1:
96 Well

※ The product indicated as "Research reagents" in the column Intended Use cannot be used
  for diagnostic nor any medical purpose.
※ The datasheet listed on this page is sample only. Please refer to the datasheet
  enclosed in the product purchased before use.

Product Overview

Product Code 27110
Product Name Human CD134/OX40 Assay Kit - IBL
Maker Name Immuno-Biological Laboratories Co., Ltd.
Intended Use Research reagents
Measuring Method ELISA
Conjugate HRP
Species Human
Measuring Samples Serum, EDTA-plasma, Cell culture supernatant
Measurement Range 15.6 - 1,000 pg/mL
Primary Reaction 120 minutes at room temperature
Secondary Reaction 60 minutes at room temperature
Sensitivity 6.3 pg/mL
Specificity Specifically detect Human CD134/OX40 in human serum, EDTA plasma and cell culture supernatant.
Storage Condition 2 - 8 ℃
Poisonous and Deleterious Substances Not Applicable
Cartagena Not Applicable
Measuring Service Available
Package Size 1 96 Well

Product Description

CD134/OX40 is a receptor of OX40 ligand (OX40L) transactivated by Tax of HTLV-1. OX40 is expressed in activated CD4+ and CD8+ T cells. OX40 on the activated T cells have a role in proliferation of T cells and production of cytokines by binding with OX40L expressed on antigen presenting cells like dendritic cells or activated B cells. In addition, it also be involved in activated T cell invasion to inflamed area through the binding with OX40L.

It has been observed that soluble CD134 / OX40 in plasma is significantly higher in acute adult T-cell leukemia patients compare to HTLV-1 carriers and healthy individual in recent study. It has also been reported that HTLV-1 gene (number of the copy) in peripheral blood lymphocytes was correlated with soluble OX40 level in plasma of HTLV-1 infected patients. (See reference below)

Tanaka Y, Takahashi Y, Tanaka R, Miyagi T, Saito M, Fukushima T. Association of high levels of plasma OX40 with acute
adult T-cell leukemia. Int J Hematol. 2019 Jan 16.

 
 

FAQ

  • Question
    Q.Is composition of EIA buffer of each ELISA kit all same? Can it be mixed to use?
    ELISA common FAQ
  • Answer
    A.No it isn't. As constitute of each EIA buffer is different, it cannot be mixed with other lots or EIA buffers contained in other kind of ELISA kits.
  • Question
    Q.What is the composition of concentrated wash buffer?
    ELISA common FAQ
  • Answer
    A.It contains ordinary Tween and phosphate buffer (0.05% Tween-20 in PB).
  • Question
    Q.What is the feature of the plate?
    ELISA common FAQ
  • Answer
    A.We use plate that is flat bottom and removable strip type plate (8wellx 12 strips).
  • Question
    Q.Can I re-use standard after reconstitution?
    ELISA common FAQ
  • Answer
    A.Not recommended to re-use standard after reconstitution. Please use it at once after the reconstitution.
    Please note that there are some exceptions. One time freeze-thaw the standard is acceptable for use after reconstitution for some ELISAs.
    Please check the details on each product datasheet.
  • Question
    Q.What is different between reagent blank and test sample blank?
    ELISA common FAQ
  • Answer
    A.Reagent blank means a well is only added EIA buffer and the purpose is confirming whether the measuring value is influenced by lack of washing process. Test sample blank means a well is added EIA buffer and HRP antibody and the purpose is to calculate the background.
  • Question
    Q.How many samples can be measured by this kit?
    ELISA common FAQ
  • Answer
    A.The pre-coated plate contained in our ELISA kit is 96 wells plate. We recommend to use 16 wells (2 slits) for standard and 80 wells (10 slits) for 40 samples in duplicate.
  • Question
    Q.What is LOD (Limit of Detection)?
    ELISA common FAQ
  • Answer
    A.It (LOD) is defined as sensitivity that is calculated using the NCCSL method. Please refer to a datasheet of each product.
  • Question
    Q.What is LOQ (Limit of Quantification)?
    ELISA common FAQ
  • Answer
    A.It (LOQ) is the lowest value of measurement (standard) range. Please refer to a datasheet of each product.

Note: This product is for research use only. It cannot be used as a diagnostic tool or drug.