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#11088 Anti-Human sAPPα (2B3) Mouse IgG MoAb

  • WB
Intended Use:
Research reagents
Application:
WB, IP
Package Size1:
100 μg
Package Size2:
10 μg
Note on Application Abbreviations
WB:Western Blotting
IP:Immunoprecipitation

※ The product indicated as "Research reagents" in the column Intended Use cannot be used
  for diagnostic nor any medical purpose.
※ The datasheet listed on this page is sample only. Please refer to the datasheet
  enclosed in the product purchased before use.

Product Overview

Product Overview

Product Code 11088
Product Name Anti-Human sAPPα (2B3) Mouse IgG MoAb
Intended Use Research reagents
Application WB, IP
Species Human
Immunizing antigen Synthetic peptide of the C-terminal part of Human sAPPα (DAEFRHDSGYEVHHQK)
Source Mouse-Mouse hybridoma (X63 - Ag 8.653 × BALB/c mouse spleen cells)
Clone Name 2B3
Subclass IgG2b
Purification Method Affinity purified with antigen peptide
Specificity Not react with sAPPb.
Not react with recombinant full-length APP by western blotting.
Cross-reacts to mouse and rat
Package Form Lyophilized product from PBS containing 1 % BSA and 0.05 % NaN3
Storage Condition 2 - 8℃
Poisonous and Deleterious Substances Applicable
Cartagena Not Applicable
Package Size 1 100 μg
Package Size 2 10 μg
Remarks1 The commercial use of products without our permission is prohibited. Please make sure to contact us and obtain permission.

Product Description

Product Description

Amyloid precursor protein (APP) is precursor protein of Amyloid β which is major constituent of senile plaque in Alzheimer's disease. It is known that there are three major isoforms, APP695, APP751 and APP770, and are generated from alternative splicing of common precursor mRNA. Processing of APP occurs by two major pathways, non-amyloidogenic pathway and amyloidogenic pathway. The non-amyloidogenic pathway is mediated by α and γ-secretases and gives rise to a large fragment known as soluble APPα (sAPPα) and a small 3 kDa peptide known as p3. On the other hand, the Amyloidogenic pathway is mediated by β- and γ-secretases and yields soluble APPβ (sAPPβ) and Amyloid β. The physiologic function of APP itself is not clear, however, it is supposed that the function of APP in neuron system is different from that in other organ. Amyloidβ is derived by the sequential cleavage of amyloid precursor protein (APP) by beta- and gamma-secretases. A double missense mutation (Lys670→Asn and Met671→Leu) in APP found in a Swedish pedigree (APPβ-sw) elevates Abeta40 and Abeta42 production, and the mutation is utilized in establishment of transgenic mice overexpress a mutant form of human amyloid precursor protein. Amyloidβproduction and, beta-secretase cleavage of APPβ-sw reportedly occur in the endoplasmic reticulum (ER), Golgi and endocytic compartments. In nerve cells, APP containing an N- or O-type sugar chain modification (mature APP) is phosphorylated at the Thr668 position (APP695) by the actions of Cdk5 and c-Jun NH2-terminal kinase (JNK), which are nerve-specifically activated, and becomes translocated to the cell membrane and neuritis. It has been considered that the phosphorylation induces structural changes in the cytoplasmic domain of APP and influences Aβ production. Regulation of the bonding of APP with FE65 is believed to be involved in information transmission.

FAQ

FAQ

  • Question
    Q.Does this antibody cross react to mouse samples?
  • Answer
    A.Cross reactivity with mouse samples is not so strong when it's compared with human samples. But we have confirmed this antibody detects mouse sAPPα.