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#27776 Human APP βCTF Assay Kit - IBL

  • #27776 Human APP βCTF ELISA Kit
  • #27776 Human APP βCTF ELISA Kit
  • #27776 Human APP βCTF ELISA Kit
  • #27776 Human APP βCTF ELISA Kit
Intended Use：
Research reagents
Measuring Method：
ELISA
Sample Types：
Human
Measuring Samples：
Cell extract
Measurement Range：
0.19 - 12 pmol/L
Package Size1：
96 Well

※ The product indicated as "Research reagents" in the column Intended Use cannot be used
　 for diagnostic nor any medical purpose.
※ The datasheet listed on this page is sample only. Please refer to the datasheet
　 enclosed in the product purchased before use.

Product Overview

Product Overview

Product Code 27776
Product Name Human APP βCTF Assay Kit - IBL
Intended Use Research reagents
Measuring Method ELISA
Conjugate HRP
Species Human
Measuring Samples Cell extract
Measurement Range 0.19 - 12 pmol/L
Primary Reaction Overnight at 2 - 8 ℃
Secondary Reaction 60 minutes at 2 - 8 ℃
Sensitivity 0.02 pmol/L
Specificity Compound Cross Reactivity
Human APP βCTF 100.0%
Human sAPPβ-Wild Type ≦0.1%
Human sAPPβ-Swedish Type ≦0.1%
Human sAPPα ≦0.1%
Storage Condition 2 - 8 ℃
Poisonous and Deleterious Substances Not Applicable
Cartagena Not Applicable
Measuring Service Not Available
Package Size 1 96 Well

Product Description

Product Description

Alzheimer’s disease (AD) was first reported by A. Alzheimer, a German neuropathologist in 1907 and is considered as a major factor of senile dementia. It is known that Amyloid β (Aβ; which is a major constituent of senile plaque) is cleaved from Amyloid Precursor Protein (APP; which exists in three main isoforms, APP695, APP751, and APP770) by β-secretase and subsequent γ-secretase. On the one hand, it has been also reported there is βCTF (β-secretase C-terminal fragment), fragments which are cleaved by β-secretase but not by γ-secretase. It is thought that measuring βCTF, in addition to Aβ, soluble APPα and APPβ, also has significance in research of the metabolic pathway of APP. This ELISA can measure human APP βCTF in samples such as cultured cell lysate.

The control set is available for sale on request. Learn More

FAQ

FAQ

  • Question
    Q.Can mouse or rat samples be used for this kit?
  • Answer
    A.Wild type mouse or rat samples cannot be used, but APP-transgenic (Tg2576) mouse can be used for the kit.
  • Question
    Q.Is there any recommended buffer solution for cell extraction?
  • Answer
    A.We recommend to use our product #19022 IBLysis.
  • Question
    Q.What is the epitope of the antibodies used in this kit?
  • Answer
    A.The epitope of HRP conjugated labeled antibody (Anti-Human Aβ (N) (82E1) Mouse IgG MoAb Affinity Purify at N-Terminal) used in this kit is DAEFRHDSGYEVHHQK.
    The epitope of the precoated plate antibody (Anti-Human APP-C Rabbit IgG Affinity Purify at C terminal) used in this kit is NGYENPTYKFFEQMQN.
  • Question
    Q.Is composition of EIA buffer of each ELISA kit all same? Can it be mixed to use?
    ELISA common FAQ
  • Answer
    A.No it isn't. As constitute of each EIA buffer is different, it cannot be mixed with other lots or EIA buffers contained in other kind of ELISA kits.
  • Question
    Q.What is the composition of concentrated wash buffer?
    ELISA common FAQ
  • Answer
    A.It contains ordinary Tween and phosphate buffer (0.05% Tween-20 in PB).
  • Question
    Q.What is the feature of the plate?
    ELISA common FAQ
  • Answer
    A.We use plate that is flat bottom and removable strip type plate (8wellx 12 strips).
  • Question
    Q.Can I re-use standard after reconstitution?
    ELISA common FAQ
  • Answer
    A.Not recommended to re-use standard after reconstitution. Please use it at once after the reconstitution.
    Please note that there are some exceptions. One time freeze-thaw the standard is acceptable for use after reconstitution for some ELISAs.
    Please check the details on each product datasheet.
  • Question
    Q.What is different between reagent blank and test sample blank?
    ELISA common FAQ
  • Answer
    A.Reagent blank means a well is only added EIA buffer and the purpose is confirming whether the Test sample value is influenced by lack of washing process or other operations. Test sample blank means a well is added EIA buffer and HRP antibody and the purpose is to calculate the background.
  • Question
    Q.How many samples can be measured by this kit?
    ELISA common FAQ
  • Answer
    A.The pre-coated plate contained in our ELISA kit is 96 wells plate. We recommend to use 16 wells (2 slits) for standard and 80 wells (10 slits) for 40 samples in duplicate.
  • Question
    Q.What is LOD (Limit of Detection)?
    ELISA common FAQ
  • Answer
    A.It (LOD) is defined as sensitivity that is calculated using the NCCSL method. Please refer to a datasheet of each product.
  • Question
    Q.What is LOQ (Limit of Quantification)?
    ELISA common FAQ
  • Answer
    A.It (LOQ) is the lowest value of measurement (standard) range. Please refer to a datasheet of each product.
  • Question
    Q.What is the definition of Over Night (O/N) reaction?
    ELISA common FAQ
  • Answer
    A.It means that the reaction is required more than 16 hours unless otherwise specifically defined it on a datasheet of each ELISA product.
  • Question
    Q.What is the specification of quality control for ELISA product release?
    ELISA common FAQ
  • Answer
    A.The information of specification is available on individual lot specific CoA. Please contact us with your reference lot number for obtaining of specific CoA.
  • Question
    Q.What is the number (e.g. 432143214321) at the edge of strips of the plate?
    ELISA common FAQ
  • Answer
    A.According to the plate maker (ThermoFisher), it does not have any specific meaning as it is just the number of molds.
  • Question
    Q.How to wash an ELISA plate?
    ELISA common FAQ
  • Answer
    A.Washing it by an auto-washer is highly recommended.
    If it is not available, please refer to the demo video (only 2 mins) using a washing bottle.
  • Question
    Q.The wells turned black during the test with the kit.
    ELISA common FAQ
  • Answer
    A.It is possible that the wells were not washed sufficiently during the washing process after the HRP-labeled antibody reaction.
    Be sure to wash the wells enough times as described in the data sheet with washing buffer of more than 350 µL.
  • Question
    Q.How do you define the acceptance range of quality control of control sets?
    Control Set Common FAQ
  • Answer
    A.In principal, it is defined as ±15%-20% based the standard value described on each datasheet.
  • Question
    Q.What is the shelf life of control set?
    Control Set Common FAQ
  • Answer
    A.The shelf life is 12 months upon the goods is dispatched from our lab if the goods is unopned.
  • Question
    Q.What is the storage condition of control sets?
    Control Set Common FAQ
  • Answer
    A.It should be stored under chilled condition (2～10℃).
  • Question
    Q.Can the control set be used after reconstitution?
    Control Set Common FAQ
  • Answer
    A.No it cannot be re-used after the reconstitution.
  • Question
    Q.What is the package form of control sets?
    Control Set Common FAQ
  • Answer
    A.It is lyophilized form.
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