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#27163 Rat GRO/CINC-3 Assay Kit - IBL

Intended Use:
Research reagents
Measuring Method:
ELISA
Sample Types:
Rat
Measuring Samples:
EDTA-plasma, Serum, Cell culture supernatant
Measurement Range:
4.69 ~ 300 pg/mL
Package Size1:
96 Well

※ The product indicated as "Research reagents" in the column Intended Use cannot be used
  for diagnostic nor any medical purpose.
※ The datasheet listed on this page is sample only. Please refer to the datasheet
  enclosed in the product purchased before use.

Product Overview

Product Overview

Product Code 27163
Product Name Rat GRO/CINC-3 Assay Kit - IBL
Intended Use Research reagents
Measuring Method ELISA
Conjugate HRP
Species Rat
Measuring Samples EDTA-plasma, Serum, Cell culture supernatant
Measurement Range 4.69 ~ 300 pg/mL
Primary Reaction 60minutes at 37 ℃
Secondary Reaction 30 minutes at 2 - 8 ℃
Sensitivity 0.51 pg/mL
Specificity Compound Cross Reactivity
Rat GRO/CINC-3 100.0%
Rat GRO/CINC-1 ≦0.1%
Rat GRO/CINC-2α ≦0.1%
Rat GRO/CINC-2β ≦0.1%
Mouse GRO/KC ≦0.1%
Storage Condition 2 - 8 ℃
Poisonous and Deleterious Substances Not Applicable
Cartagena Not Applicable
Measuring Service Not Available
Package Size 1 96 Well

Product Description

Product Description

Cytokine-induced neutrophil chemo attractant 3(CINC-3) is, also known as rat MIP-
2, a member of the alpha (CXC) subfamily of chemokines, which is expressed by
cytokine-stimulated rat alveolar macrophages and fibroblasts. The amino acid
sequence of rat CINC-3 is 88% identical to murine MIP-2. CINC-3 is also known to
be a potent chemo tactic factor for rat neutrophil both in vitro and in vivo.
Nakagawa’s group at Toyama Medical and Pharmaceutical Univ. in 1994 has
postulated that rat neutrophil have at least two classes of CINC receptors, a class of CINC-3 specific ones and a second common receptor shared by all CINCs.

FAQ

FAQ

  • Question
    Q.Is composition of EIA buffer of each ELISA kit all same? Can it be mixed to use?
    ELISA common FAQ
  • Answer
    A.No it isn't. As constitute of each EIA buffer is different, it cannot be mixed with other lots or EIA buffers contained in other kind of ELISA kits.
  • Question
    Q.What is the composition of concentrated wash buffer?
    ELISA common FAQ
  • Answer
    A.It contains ordinary Tween and phosphate buffer (0.05% Tween-20 in PB).
  • Question
    Q.What is the feature of the plate?
    ELISA common FAQ
  • Answer
    A.We use plate that is flat bottom and removable strip type plate (8wellx 12 strips).
  • Question
    Q.Can I re-use standard after reconstitution?
    ELISA common FAQ
  • Answer
    A.Not recommended to re-use standard after reconstitution. Please use it at once after the reconstitution.
    Please note that there are some exceptions. One time freeze-thaw the standard is acceptable for use after reconstitution for some ELISAs.
    Please check the details on each product datasheet.
  • Question
    Q.What is different between reagent blank and test sample blank?
    ELISA common FAQ
  • Answer
    A.Reagent blank means a well is only added EIA buffer and the purpose is confirming whether the measuring value is influenced by lack of washing process. Test sample blank means a well is added EIA buffer and HRP antibody and the purpose is to calculate the background.
  • Question
    Q.How many samples can be measured by this kit?
    ELISA common FAQ
  • Answer
    A.The pre-coated plate contained in our ELISA kit is 96 wells plate. We recommend to use 16 wells (2 slits) for standard and 80 wells (10 slits) for 40 samples in duplicate.
  • Question
    Q.What is LOD (Limit of Detection)?
    ELISA common FAQ
  • Answer
    A.It (LOD) is defined as sensitivity that is calculated using the NCCSL method. Please refer to a datasheet of each product.
  • Question
    Q.What is LOQ (Limit of Quantification)?
    ELISA common FAQ
  • Answer
    A.It (LOQ) is the lowest value of measurement (standard) range. Please refer to a datasheet of each product.
  • Question
    Q.What is the definition of Over Night (O/N) reaction?
  • Answer
    A.It means that the reaction is required more than 16 hours unless otherwise specifically defined it on a datasheet of each ELISA product.

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