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#27162 Rat GRO/CINC-1 Assay Kit - IBL
- Intended Use:
- Research reagents
- Measuring Method:
- ELISA
- Sample Types:
- Rat
- Measuring Samples:
- EDTA-plasma, Serum, Cell culture supernatant
- Measurement Range:
- 4.69 ~ 300 pg/mL
- Package Size1:
- 96 Well
※ The product indicated as "Research reagents" in the column Intended Use cannot be used
for diagnostic nor any medical purpose.
※ The datasheet listed on this page is sample only. Please refer to the datasheet
enclosed in the product purchased before use.
Product Overview
Product Overview
Product Code | 27162 |
---|---|
Product Name | Rat GRO/CINC-1 Assay Kit - IBL |
Intended Use | Research reagents |
Measuring Method | ELISA |
Conjugate | HRP |
Species | Rat |
Measuring Samples | EDTA-plasma, Serum, Cell culture supernatant |
Measurement Range | 4.69 ~ 300 pg/mL |
Primary Reaction | 60minutes at 37 ℃ |
Secondary Reaction | 30 minutes at 37 ℃ |
Sensitivity | 0.35 pg/mL |
Specificity | Compound Cross Reactivity Rat GRO/CINC-1 100.0% Rat GRO/CINC-2α ≦0.1% Rat GRO/CINC-2β ≦0.1% Rat GRO/CINC-3 ≦0.1% Mouse GROβ/MIP-2 ≦0.1% |
Storage Condition | 2 - 8 ℃ |
Poisonous and Deleterious Substances | Not Applicable |
Cartagena | Not Applicable |
Measuring Service | Not Available |
Package Size 1 | 96 Well |
Product Description
Product Description
GRO (Growth Related Oncogene) belonging to IL-8 family is polypeptide which has three isoforms α, β and γ, and it inhibits proliferation of endothelial cells. GRO/CINC-1 (cytokine-induced neutrophil chemo attractant 1) was originally purified from media conditioned by IL-1β stimulated rat kidney epithelioid cells (NRK-52E.) Amino acid sequence that encodes rat CINC-1 was identified in 1989 by Watanabe’s group at Toyama Medical and Pharmaceutical University. CINC-1 is a member of the alpha (CXC) subfamily of chemokines. Three additional rat CXC chemokines (CINC-2α, CINC-2β, CINC-3/MIP-2) have been identified. The protein sequence of CINC-1 is 63 - 67% identical to that of CINC-2α, CINC-2β and CINC-3/MIP-2. In addition, each of GROα, GROβ and GROγ is sharing 68%, 71% and 69% identity with CINC-1. This has been suggested that CINCs are the rat counterparts of human GROs. GROα/MGSA has a high homology with IL-8 in amino acid sequences. It has been reported that it also has a similar configuration in 3D structural analysis and the almost same level of leukocyte chemotaxis with IL-8. Also its production from activated monocyte, fibroblast, epithelial and endothelial cells has been reported. It has been reported that GROβ inhibits angiogenesis in case of administration of GRO in vivo. Mouse KC (Keratinocyto-derived Cytokine) is a counterpart of human GRO protein and it has been reported that KC is produced in such as activated monocyte and fibroblast. Mouse MIP (Macrophage Inflammatory Protein) is polypeptide which has leukocyte chemotaxis and it originally was discovered as a small protein (molecular weight about 8 kDa) induced by the lipo-polysaccharide stimulation in the macrophage cell line. Two kinds, MIP-1 and MIP-2 are identified by the research afterwards.
The control set is available for sale on request. Learn More
The control set is available for sale on request. Learn More
References
References
- Tumor necrosis factor-alpha-induced cytokine-induced neutrophil chemoattractant-1 (CINC-1) production by rat gastric epithelial cells: role of reactive oxygen species and nuclear factor-kappaB. Handa O J Pharmacol Exp Ther. 2004 May;309(2):670-6.PMID: 14747614
- Octreotide regulates CC but not CXC LPS-induced chemokine secretion in rat Kupffer cells. Valatas V Br J Pharmacol. 2004 Feb;141(3):477-87.PMID: 14718256
- Different actions of endothelin-1 on chemokine production in rat cultured astrocytes: reduction of CX3CL1/fractalkine and an increase in CCL2/MCP-1 and CXCL1/CINC-1. Koyama Y J Neuroinflammation. 2013 Apr 30;10:51.PMID: 23627909
- Differential production of MCP-1 and cytokine-induced neutrophil chemoattractant in the ischemic brain after transient focal ischemia in rats Shinsuke Yamagami et al. June 1999 Journal of Leukocyte Biology vol. 65 no. 6 744-749PMID: 10380894
- Role of nuclear factor-κB in gastric ulcer healing in rats Takahashi S et al. Am J Physiol Gastrointest Liver Physiol. 2001 Jun;280(6):G1296-304.PMID: 11352824
- Effects of granulocyte colony-stimulating factor (G-CSF) and neutrophil elastase inhibitor (ONO-5046) on acid-induced lung injury in rats. Jian MY et al. Inflammation. 2004 Dec;28(6):327-36.PMID: 16245075
- Effects of long-term administration of erythromycin on cytokine production in rat alveolar macrophages. Sugiyama Y et al. Eur Respir J. 1999 Nov;14(5):1113-6.PMID: 10596699
- Lansoprazole ameliorates intestinal mucosal damage induced by ischemia-reperfusion in rats. Ichikawa H et al. World J Gastroenterol. 2004 Oct 1;10(19):2814-7.PMID: 15334676
- Cytokine-induced neutrophil chemoattractants in healing of gastric ulcers in rats: expression of >40-kDa chemoattractant in delayed ulcer healing by indomethacin. Yamada H et al. Dig Dis Sci. 1999 May;44(5):889-95.PMID: 10235592
- Effect of a novel water-soluble vitamin E derivative as a cure for TNBS-induced colitis in rats. Isozaki Y et al. Int J Mol Med. 2006 Mar;17(3):497-502.PMID: 16465398
Note: Retrieve by PMID number in displayed by abstract: http://www.ncbi.nlm.nih.gov
FAQ
FAQ
-
Q.Can this kit be used for heparin-plasma samples?
-
A.We do not recommend using the kit for heparin plasma samples.
-
Q.Is composition of EIA buffer of each ELISA kit all same? Can it be mixed to use?
ELISA common FAQ -
A.No it isn't. As constitute of each EIA buffer is different, it cannot be mixed with other lots or EIA buffers contained in other kind of ELISA kits.
-
Q.What is the composition of concentrated wash buffer?
ELISA common FAQ -
A.It contains ordinary Tween and phosphate buffer (0.05% Tween-20 in PB).
-
Q.What is the feature of the plate?
ELISA common FAQ -
A.We use plate that is flat bottom and removable strip type plate (8wellx 12 strips).
-
Q.Can I re-use standard after reconstitution?
ELISA common FAQ -
A.Not recommended to re-use standard after reconstitution. Please use it at once after the reconstitution.
Please note that there are some exceptions. One time freeze-thaw the standard is acceptable for use after reconstitution for some ELISAs.
Please check the details on each product datasheet. -
Q.What is different between reagent blank and test sample blank?
ELISA common FAQ -
A.Reagent blank means a well is only added EIA buffer and the purpose is confirming whether the Test sample value is influenced by lack of washing process or other operations. Test sample blank means a well is added EIA buffer and HRP antibody and the purpose is to calculate the background.
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Q.How many samples can be measured by this kit?
ELISA common FAQ -
A.The pre-coated plate contained in our ELISA kit is 96 wells plate. We recommend to use 16 wells (2 slits) for standard and 80 wells (10 slits) for 40 samples in duplicate.
-
Q.What is LOD (Limit of Detection)?
ELISA common FAQ -
A.It (LOD) is defined as sensitivity that is calculated using the NCCSL method. Please refer to a datasheet of each product.
-
Q.What is LOQ (Limit of Quantification)?
ELISA common FAQ -
A.It (LOQ) is the lowest value of measurement (standard) range. Please refer to a datasheet of each product.
-
Q.What is the definition of Over Night (O/N) reaction?
ELISA common FAQ -
A.It means that the reaction is required more than 16 hours unless otherwise specifically defined it on a datasheet of each ELISA product.
-
Q.What is the specification of quality control for ELISA product release?
ELISA common FAQ -
A.The information of specification is available on individual lot specific CoA. Please contact us with your reference lot number for obtaining of specific CoA.
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Q.What is the number (e.g. 432143214321) at the edge of strips of the plate?
ELISA common FAQ -
A.According to the plate maker (ThermoFisher), it does not have any specific meaning as it is just the number of molds.
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Q.How to wash an ELISA plate?
ELISA common FAQ -
A.Washing it by an auto-washer is highly recommended.
If it is not available, please refer to the demo video (only 2 mins) using a washing bottle. -
Q.The wells turned black during the test with the kit.
ELISA common FAQ -
A.It is possible that the wells were not washed sufficiently during the washing process after the HRP-labeled antibody reaction.
Be sure to wash the wells enough times as described in the data sheet with washing buffer of more than 350 µL. -
Q.How do you define the acceptance range of quality control of control sets?
Control Set Common FAQ -
A.In principal, it is defined as ±15%-20% based the standard value described on each datasheet.
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Q.What is the shelf life of control set?
Control Set Common FAQ -
A.The shelf life is 12 months upon the goods is dispatched from our lab if the goods is unopned.
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Q.What is the storage condition of control sets?
Control Set Common FAQ -
A.It should be stored under chilled condition (2~10℃).
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Q.Can the control set be used after reconstitution?
Control Set Common FAQ -
A.No it cannot be re-used after the reconstitution.
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Q.What is the package form of control sets?
Control Set Common FAQ -
A.It is lyophilized form.