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#17165 Endothelin-1 Assay Kit - IBL

  • Correlation Data with #27165
Intended Use:
Research reagents
Measuring Method:
ELISA
Sample Types:
Human
Measuring Samples:
EDTA-plasma, Serum, Tissue extract, Cell culture supernatant
Measurement Range:
0.78 ~ 100 pg/mL
Package Size1:
96 Well

※ The product indicated as "Research reagents" in the column Intended Use cannot be used
  for diagnostic nor any medical purpose.
※ The datasheet listed on this page is sample only. Please refer to the datasheet
  enclosed in the product purchased before use.

Product Overview

Product Overview

Product Code 17165
Product Name Endothelin-1 Assay Kit - IBL
Intended Use Research reagents
Measuring Method ELISA
Conjugate HRP
Species Human
Measuring Samples EDTA-plasma, Serum, Tissue extract, Cell culture supernatant
Measurement Range 0.78 ~ 100 pg/mL
Primary Reaction Overnight at 2 - 8 ℃
Secondary Reaction 30 minutes at 37 ℃
Sensitivity 0.39 pg/mL
Specificity Substance Cross reactivity (%)
Endothelin-1(1-21) 100.0%
Endothelin-2(1-21) 3.32%
Endothelin-3(1-21) ≦0.1
Endothelin-1(1-31) 0.16%
Endothelin-2(1-31) ≦0.1
Endothelin-3(1-31) ≦0.1
Big Endothelin-1 ≦0.1
Big Endothelin-2 ≦0.1
Big Endothelin-3 ≦0.1
VIC(Mouse Endothelin-2) ≦0.1
Rat Big Endothelin-1 0.1%
Storage Condition ー20℃
Poisonous and Deleterious Substances Not Applicable
Cartagena Not Applicable
Measuring Service Not Available
Package Size 1 96 Well

Product Description

Product Description

Endothelins (ETs) are isopeptides produced by vascular endothelium having potent vasoconstriction activity. The peptides are encoded by three separate genes and processed to yield 39 residue Big Endothelin (Big ET) molecule, which are further processed to the 21 amino acid sequences termed Endothelin-1 (ET-1), Endothelin-2 (ET-2) and Endothelin-3 (ET-3). All of members of the endothelin family contain two essential disulfide bridges and size conserved amino acid residues at the C-terminus. The ETs are produced by a variety of issues in vivo, including lung, kidney, brain, pituitary and placenta.

FAQ

FAQ

  • Question
    Q.Can this kit be used for heparin-plasma samples?
  • Answer
    A.Endothelin-1 in heparin plasma can be measured. However, the extraction process must be performed. For details about the extraction process, see “12. Endothelin extraction process” on page 2 of the datasheet. Please refer to the datasheet for additional information.
  • Question
    Q.What kind of animal samples can be used with this kit?
  • Answer
    A.Human, rat, pig, canine, mouse, cow, rabbit samples can be used with the kit as epitope of No.1 to 21 of Endothelin-1 is same among the species.
  • Question
    Q.In regards to the extraction of the plasma sample, can I use a column other than C18?
  • Answer
    A.Please ask the maker for the column you intend to use.
  • Question
    Q.What kind of Sep-pak should be used for the assay?
  • Answer
    A.We use Sep-pak light.
  • Question
    Q.Is a correlation data with #27165 available?
  • Answer
    A.Yes, it is. The data is listed on the product detail page as a photo image. Please refer to the data.
  • Question
    Q.Can I use "reconstituted labeled antibody" after re-stored at -20℃?
  • Answer
    A.Not recommended. Please use labeled antibody immediately after the reconstitution.
  • Question
    Q.Is composition of EIA buffer of each ELISA kit all same? Can it be mixed to use?
    ELISA common FAQ
  • Answer
    A.No it isn't. As constitute of each EIA buffer is different, it cannot be mixed with other lots or EIA buffers contained in other kind of ELISA kits.
  • Question
    Q.What is the composition of concentrated wash buffer?
    ELISA common FAQ
  • Answer
    A.It contains ordinary Tween and phosphate buffer (0.05% Tween-20 in PB).
  • Question
    Q.What is the feature of the plate?
    ELISA common FAQ
  • Answer
    A.We use plate that is flat bottom and removable strip type plate (8wellx 12 strips).
  • Question
    Q.Can I re-use standard after reconstitution?
    ELISA common FAQ
  • Answer
    A.Not recommended to re-use standard after reconstitution. Please use it at once after the reconstitution.
    Please note that there are some exceptions. One time freeze-thaw the standard is acceptable for use after reconstitution for some ELISAs.
    Please check the details on each product datasheet.
  • Question
    Q.What is different between reagent blank and test sample blank?
    ELISA common FAQ
  • Answer
    A.Reagent blank means a well is only added EIA buffer and the purpose is confirming whether the measuring value is influenced by lack of washing process. Test sample blank means a well is added EIA buffer and HRP antibody and the purpose is to calculate the background.
  • Question
    Q.How many samples can be measured by this kit?
    ELISA common FAQ
  • Answer
    A.The pre-coated plate contained in our ELISA kit is 96 wells plate. We recommend to use 16 wells (2 slits) for standard and 80 wells (10 slits) for 40 samples in duplicate.
  • Question
    Q.What is LOD (Limit of Detection)?
    ELISA common FAQ
  • Answer
    A.It (LOD) is defined as sensitivity that is calculated using the NCCSL method. Please refer to a datasheet of each product.
  • Question
    Q.What is LOQ (Limit of Quantification)?
    ELISA common FAQ
  • Answer
    A.It (LOQ) is the lowest value of measurement (standard) range. Please refer to a datasheet of each product.
  • Question
    Q.What is the definition of Over Night (O/N) reaction?
  • Answer
    A.It means that the reaction is required more than 16 hours unless otherwise specifically defined it on a datasheet of each ELISA product.

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