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#27601 Mouse soluble α-Klotho Assay Kit - IBL

  • Stability Test (Effect of Freeze & Thaw)
  • Stability Test (Room Temperature)
  • (Experiment Example) BALB/C Mouse N=7, Urine sample Weekly age 4, 12, 22 weeks
  • 27601 Mouse soluble α-Klotho ELISA Kit
  • 27601 Mouse soluble α-Klotho ELISA Kit
  • 27601 Mouse soluble α-Klotho ELISA Kit
  • 27601 Mouse soluble α-Klotho ELISA Kit
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Intended Use:
Research reagents
Measuring Method:
ELISA
Sample Types:
Mouse
Measuring Samples:
Urine
Measurement Range:
78.13 ~ 5000 pg/mL
Package Size1:
96 Well

※ The product indicated as "Research reagents" in the column Intended Use cannot be used
for diagnostic nor any medical purpose.

Product Overview

Product Code 27601
Product Name Mouse soluble α-Klotho Assay Kit - IBL
Maker Name Immuno-Biological Laboratories Co., Ltd.
Intended Use Research reagents
Measuring Method ELISA
Conjugate HRP
Species Mouse
Measuring Samples Urine
Measurement Range 78.13 ~ 5000 pg/mL
Primary Reaction 60 minutes at 37 ℃
Secondary Reaction 30 minutes at 2 - 8 ℃
Sensitivity 18.06 pg/mL
Specificity Mouse soluble α-Klotho 100%
Mouse soluble α-Klotho(KL1) 100%
Mouse soluble β-Klotho ≦0.1%
Human soluble α-Klotho ≦0.1%
Rat soluble α-Klotho N.T
Storage Condition 2 - 8 ℃
Poisonous and Deleterious Substances Not Applicable
Cartagena Not Applicable
Measuring Service Available
Package Size 1 96 Well

Product Description

α-klotho (Alpha Klotho) was identified as an extremely down-regulated gene in the genetically-modified mouse of which phenotype is very similar to various symptoms of human aging. Then, the sequences of Alpha Klotho genes have been identified in various species including humans based on the one of mouse. Alpha Klotho protein is a 130 kDa, one-transmembrane protein and its expression is confirmed in the kidneys and the parathyroid glands. In recent years, it has become clear that Alpha Klotho is an important molecule within a living organism regulating the metabolism of mineral such as calcium and phosphorus. Therefore, it is considered that, in Alpha Klotho mouse, early aging-like symptoms are induced by impaired mineral homeostasis caused by decreased expression of Alpha Klotho. Meanwhile, it is reported that the long N-terminal extracellular domain which comprises the major portion of sequence of Alpha Klotho protein is released free into blood by shedding. However, there are many unclear points about functions and changes in concentration of free (soluble) Alpha Klotho protein, so it has been required to develop the measurement system of Alpha Klotho. This ELISA kit can measure mouse soluble Alpha Klotho protein in mouse urine.

The control set is available for sale on request. Learn More

Keywords:
α-Klotho / Alpha Klotho / FGF23 / Kidney disease / Chronic Kidoeny disease (CKD) / Mineral Bone Disease (MBD) / Osteoporosis / Aging

FAQ

  • Question
    Q.What is recommended dilution ratio?
  • Answer
    A.8 fold dilution is acceptable for the measurement, however, we recommend to dilute mouse urine sample more than 16 fold to avoid the data becomes range over.
  • Question
    Q.Can rat sample be used for this kit?
  • Answer
    A.No, it cannot.
  • Question
    Q.Is stability data available?
  • Answer
    A.It is available on the following relevant product page as images.
    http://www.ibl-japan.co.jp/en/search/product/detail/id=4678
  • Question
    Q.Can this kit measure mouse blood sample?
  • Answer
    A.No, it cannot.
  • Question
    Q.Is composition of EIA buffer of each ELISA kit all same? Can it be mixed to use?
    ELISA common FAQ
  • Answer
    A.No it isn't. As constitute of each EIA buffer is different, it cannot be mixed with other lots or EIA buffers contained in other kind of ELISA kits.
  • Question
    Q.What is the composition of concentrated wash buffer?
    ELISA common FAQ
  • Answer
    A.It contains ordinary Tween and phosphate buffer (0.05% Tween-20 in PB).
  • Question
    Q.What is the feature of the plate?
    ELISA common FAQ
  • Answer
    A.We use plate that is flat bottom and removable strip type plate (8wellx 12 strips).
  • Question
    Q.Can I re-use standard after reconstitution?
    ELISA common FAQ
  • Answer
    A.Not recommended to re-use standard after reconstitution. Please use it at once after the reconstitution.
    Please note that there are some exceptions. One time freeze-thaw the standard is acceptable for use after reconstitution for some ELISAs.
    Please check the details on each product datasheet.
  • Question
    Q.What is different between reagent blank and test sample blank?
    ELISA common FAQ
  • Answer
    A.Reagent blank means a well is only added EIA buffer and the purpose is confirming whether the measuring value is influenced by lack of washing process. Test sample blank means a well is added EIA buffer and HRP antibody and the purpose is to calculate the background.
  • Question
    Q.How many samples can be measured by this kit?
    ELISA common FAQ
  • Answer
    A.The pre-coated plate contained in our ELISA kit is 96 wells plate. We recommend to use 16 wells (2 slits) for standard and 80 wells (10 slits) for 40 samples in duplicate.
  • Question
    Q.What is LOD (Limit of Detection)?
    ELISA common FAQ
  • Answer
    A.It (LOD) is defined as sensitivity that is calculated using the NCCSL method. Please refer to a datasheet of each product.
  • Question
    Q.What is LOQ (Limit of Quantification)?
    ELISA common FAQ
  • Answer
    A.It (LOQ) is the lowest value of measurement (standard) range. Please refer to a datasheet of each product.
  • Question
    Q.Do you have any protocol for preparation of mice and/or rat brain
    tissue extracts?
    (ELISA common FAQ * Specified FAQ for ELISA that is applicable for brain tissue extract.)
  • Answer
    A.CHAPS is recommended for extractin of wild type (W.T.) mice or rat brain tissue.
    Please refer to the following link for the brain tissue extraction process using CHAPS.
    http://www.ibl-japan.co.jp/files/topics/4944_ext_02_0.pdf

    Guanidine solution is recommended for extraction of Transgenic (Tg) mice or rat brain tissue.
    Please refer to the following link and the following two scientific articles for brain tissue extraction process using Guanidine.
    http://www.ibl-japan.co.jp/files/topics/4945_ext_02_0.pdf

    Presenilin-2 mutation causes early amyloid accumulation and memory impairment in a transgenic mouse model of Alzheimer's disease. Toda T et al. J Biomed Biotechnol. 2011;2011:617974.
    https://www.ncbi.nlm.nih.gov/pubmed/?term=21234330

    Methylene blue modulates β-secretase, reverses cerebral amyloidosis, and improves cognition in transgenic mice. Mori T et al. J Biol Chem. 2014 Oct 31;289(44):30303-17.
    https://www.ncbi.nlm.nih.gov/pubmed/?term=25157105

    * The ELISA kit #27712 Aβ42 and #27714 Aβ40 have been upgraded to #27719 Aβ (1-42)(FL) and #27718 Aβ (1-40)(FL).

Note: This product is for research use only. It cannot be used as a diagnostic tool or drug.