#27745 Human ANGPTL2 Assay Kit - IBL

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Intended Use：: Research reagents

Measuring Method：: ELISA

Sample Types：: Human

Measuring Samples：: Serum, EDTA-plasma, Cell culture supernatant

Measurement Range：: 0.05 - 3.5 ng/mL

Package Size1：: 96 Well

※ The product indicated as "Research reagents" in the column Intended Use cannot be used
　 for diagnostic nor any medical purpose.
※ The datasheet listed on this page is sample only. Please refer to the datasheet
　 enclosed in the product purchased before use.

Product Overview

Product Code	27745
Product Name	Human ANGPTL2 Assay Kit - IBL
Intended Use	Research reagents
Measuring Method	ELISA
Conjugate	HRP
Species	Human
Measuring Samples	Serum, EDTA-plasma, Cell culture supernatant
Measurement Range	0.05 - 3.5 ng/mL
Primary Reaction	60 minutes at 37 ℃
Secondary Reaction	30 minutes at 2 - 8 ℃
Sensitivity	0.01 ng/mL
Specificity	Compound Cross-Reactivity Human ANGPTL2 100.0% Human ANGPTL3 < 0.1% Human ANGPTL4 < 0.1% Human ANGPTL6 < 0.1%
Storage Condition	2 - 8 ℃
Poisonous and Deleterious Substances	Not Applicable
Cartagena	Not Applicable
Measuring Service	Not Available
Package Size 1	96 Well

Product Description

Angiopoietin-like proteins (ANGPTLs) are secretory form proteins which are similar in structure to angiopoietin which is an angiogenesis factor, and 7 ANGPTLs have been identified. ANGPTL2 has been found to affect vascular cells and monocytes, and it have also been known that ANGPTL3 and ANGPTL4 play important roles in llipid metabolism and AGF (Angiopoietin-like growth factor)/ANGPTL6 plays in energy metabolism. These various biological effects of ANGPTL family are attracting attention as a new target of strategy against lifestyle-related diseases like metabolic syndrome or cancer. There is a report that high level of ANGPTL2 in blood observed in individuals who suffer adipsity, strong insulin resistance, diabetes and arteriosclerosis. This kit is a quantitative ELISA kit which can measure ANGPTL2 concentration in human serum or plasma.

【References】
Acute effects of statin on reduction of angiopoietin-like 2 and glyceraldehyde-derived advanced glycation end-products levels in patients with acute myocardial infarction: a message from SAMIT (Statin for Acute Myocardial Infarction Trial). Shimomura M et al. Heart Vessels. 2016 Oct;31(10):1583-9.

Association between Maternal Serum Concentrations of Angiopoietin-like Protein 2 in Early Pregnancy and Subsequent Risk of Gestational Diabetes Mellitus. Zhang Y et al. Chin Med J (Engl). 2016 Oct 5;129(19):2308-12.

Serum Angiopoietin-Like Protein 2 Concentrations Are Independently Associated with Heart Failure. Huang CL et al. PLoS One. 2015 Sep 23;10(9):e0138678.

Association of serum angiopoietin-like protein 2 with carotid intima-media thickness in subjects with type 2 diabetes. Jung CH et al. Cardiovasc Diabetol. 2015 Apr 15;14:35.

Elevated serum angiopoietin-like protein 2 correlates with the metastatic properties of colorectal cancer: a serum biomarker for early diagnosis and recurrence. Toiyama Y et al. Clin Cancer Res. 2014 Dec 1;20(23):6175-86.

References

Note: Retrieve by PMID number in displayed by abstract: http://www.ncbi.nlm.nih.gov

FAQ

Q.What is the immunogen?
A.The immunogen is recombinant the protein of ANGPTL2.
Q.Is composition of EIA buffer of each ELISA kit all same? Can it be mixed to use?
ELISA common FAQ
A.No it isn't. As constitute of each EIA buffer is different, it cannot be mixed with other lots or EIA buffers contained in other kind of ELISA kits.
Q.What is the composition of concentrated wash buffer?
ELISA common FAQ
A.It contains ordinary Tween and phosphate buffer (0.05% Tween-20 in PB).
Q.What is the feature of the plate?
ELISA common FAQ
A.We use plate that is flat bottom and removable strip type plate (8wellx 12 strips).
Q.Can I re-use standard after reconstitution?
ELISA common FAQ
A.Not recommended to re-use standard after reconstitution. Please use it at once after the reconstitution.
Please note that there are some exceptions. One time freeze-thaw the standard is acceptable for use after reconstitution for some ELISAs.
Please check the details on each product datasheet.
Q.What is different between reagent blank and test sample blank?
ELISA common FAQ
A.Reagent blank means a well is only added EIA buffer and the purpose is confirming whether the Test sample value is influenced by lack of washing process or other operations. Test sample blank means a well is added EIA buffer and HRP antibody and the purpose is to calculate the background.
Q.How many samples can be measured by this kit?
ELISA common FAQ
A.The pre-coated plate contained in our ELISA kit is 96 wells plate. We recommend to use 16 wells (2 slits) for standard and 80 wells (10 slits) for 40 samples in duplicate.
Q.What is LOD (Limit of Detection)?
ELISA common FAQ
A.It (LOD) is defined as sensitivity that is calculated using the NCCSL method. Please refer to a datasheet of each product.
Q.What is LOQ (Limit of Quantification)?
ELISA common FAQ
A.It (LOQ) is the lowest value of measurement (standard) range. Please refer to a datasheet of each product.
Q.What is the definition of Over Night (O/N) reaction?
ELISA common FAQ
A.It means that the reaction is required more than 16 hours unless otherwise specifically defined it on a datasheet of each ELISA product.
Q.What is the specification of quality control for ELISA product release?
ELISA common FAQ
A.The information of specification is available on individual lot specific CoA. Please contact us with your reference lot number for obtaining of specific CoA.
Q.What is the number (e.g. 432143214321) at the edge of strips of the plate?
ELISA common FAQ
A.According to the plate maker (ThermoFisher), it does not have any specific meaning as it is just the number of molds.
Q.How to wash an ELISA plate?
ELISA common FAQ
A.Washing it by an auto-washer is highly recommended.
If it is not available, please refer to the demo video (only 2 mins) using a washing bottle.
Q.The wells turned black during the test with the kit.
ELISA common FAQ
A.It is possible that the wells were not washed sufficiently during the washing process after the HRP-labeled antibody reaction.
Be sure to wash the wells enough times as described in the data sheet with washing buffer of more than 350 µL.