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#27182 Human EL Full-Length Assay Kit - IBL

  • #27182 Human EL Full-Length ELISA Kit
  • #27182 Human EL Full-Length ELISA Kit
  • #27182 Human EL Full-Length ELISA Kit
  • #27182 Human EL Full-Length ELISA Kit
  • #27182 Human EL Full-Length ELISA Kit
  • #27182 Human EL Full-Length ELISA Kit
  • #27182 Human EL Full-Length ELISA Kit
  • #27182 Human EL Full-Length ELISA Kit
  • #27182 Human EL Full-Length ELISA Kit
  • #27182 Human EL Full-Length ELISA Kit
  • #27182 Human EL Full-Length ELISA Kit
Intended Use:
Research reagents
Measuring Method:
ELISA
Sample Types:
Human
Measuring Samples:
EDTA-plasma, Serum, Cell culture supernatant
Measurement Range:
31.25 - 2,000 pg/mL
Package Size1:
96 Well

※ The product indicated as "Research reagents" in the column Intended Use cannot be used
  for diagnostic nor any medical purpose.
※ The datasheet listed on this page is sample only. Please refer to the datasheet
  enclosed in the product purchased before use.

Product Overview

Product Overview

Product Code 27182
Product Name Human EL Full-Length Assay Kit - IBL
Maker Name Immuno-Biological Laboratories Co., Ltd.
Intended Use Research reagents
Measuring Method ELISA
Conjugate HRP
Species Human
Measuring Samples EDTA-plasma, Serum, Cell culture supernatant
Measurement Range 31.25 - 2,000 pg/mL
Primary Reaction Overnight at 2 - 8 ℃
Secondary Reaction 30 minutes at 2 - 8 ℃
Sensitivity 5.7 pg/mL
Specificity Substance Cross Reactivity
Human EL Full-Length 100%
Human HTGL < 0.1 %
Human LPL < 0.1 %
Storage Condition 2 - 8 ℃
Poisonous and Deleterious Substances Not Applicable
Cartagena Not Applicable
Measuring Service Available
Package Size 1 96 Well
Remarks1 Full length (Active form) EL is extremely unstable protein. Collected samples shall be stored at 2 ~ 8℃ immediately after centrifugation and shall be measured within 6 hours after the collection. The samples shall not be frozen or left at room temperature as the measurement value become much lower than the value measured flesh samples.

Product Description

Product Description

Endothelial Lipase (EL) is a metabolic enzyme of HDL. It is phospholipase A1 molecule that is high substrate specificity against to phospholipid that exists in high density lipoprotein, HDL molecule and it promotes HDL metabolism through metabolizing HDL phospholipid.  It has been considered that EL involves with lipid metabolism and elevating risk of arteriosclerosis as strong expression of EL on newly formed vessel, macrophage in arteriosclerotic lesions is observed while EL expresses in vascular endothelial cells and vascular smooth myocytes of normal blood vessels.  It has been reported that both full length EL (active form) and cleaved EL (inactive forms, 40K fragment cleaved at N terminal and 28K fragment cleaved at C terminal cleaved by proprotein convertase) exist in blood. This ELISA kit is designed by using two antibodies that detects N-terminal fragment and C terminal fragment so that it can detect full length EL in serum and EDTA-plasma regardless with or without administration of heparin injection that is conventionally required for the measurement.

The control set is available for sale on request. Learn More


Keywords:
Lipase / Lipid Metabolism / Dyslipidemia

FAQ

FAQ

  • Question
    Q.How can I store the standard after reconstitute it?
  • Answer
    A.It cannot be recommended to be stored after reconstitution as it is designed for use one-off. However, if you need to reuse it due to uncontrolable situation, please store it in frozen.
  • Question
    Q.Is composition of EIA buffer of each ELISA kit all same? Can it be mixed to use?
    ELISA common FAQ
  • Answer
    A.No it isn't. As constitute of each EIA buffer is different, it cannot be mixed with other lots or EIA buffers contained in other kind of ELISA kits.
  • Question
    Q.What is the composition of concentrated wash buffer?
    ELISA common FAQ
  • Answer
    A.It contains ordinary Tween and phosphate buffer (0.05% Tween-20 in PB).
  • Question
    Q.What is the feature of the plate?
    ELISA common FAQ
  • Answer
    A.We use plate that is flat bottom and removable strip type plate (8wellx 12 strips).
  • Question
    Q.Can I re-use standard after reconstitution?
    ELISA common FAQ
  • Answer
    A.Not recommended to re-use standard after reconstitution. Please use it at once after the reconstitution.
    Please note that there are some exceptions. One time freeze-thaw the standard is acceptable for use after reconstitution for some ELISAs.
    Please check the details on each product datasheet.
  • Question
    Q.What is different between reagent blank and test sample blank?
    ELISA common FAQ
  • Answer
    A.Reagent blank means a well is only added EIA buffer and the purpose is confirming whether the measuring value is influenced by lack of washing process. Test sample blank means a well is added EIA buffer and HRP antibody and the purpose is to calculate the background.
  • Question
    Q.How many samples can be measured by this kit?
    ELISA common FAQ
  • Answer
    A.The pre-coated plate contained in our ELISA kit is 96 wells plate. We recommend to use 16 wells (2 slits) for standard and 80 wells (10 slits) for 40 samples in duplicate.
  • Question
    Q.What is LOD (Limit of Detection)?
    ELISA common FAQ
  • Answer
    A.It (LOD) is defined as sensitivity that is calculated using the NCCSL method. Please refer to a datasheet of each product.
  • Question
    Q.What is LOQ (Limit of Quantification)?
    ELISA common FAQ
  • Answer
    A.It (LOQ) is the lowest value of measurement (standard) range. Please refer to a datasheet of each product.
  • Question
    Q.What is the definition of Over Night (O/N) reaction?
    ELISA common FAQ
  • Answer
    A.It means that the reaction is required more than 16 hours unless otherwise specifically defined it on a datasheet of each ELISA product.
  • Question
    Q.What is the specification of quality control for ELISA product release?
    ELISA common FAQ
  • Answer
    A.The information of specification is available on individual lot specific CoA. Please contact us with your reference lot number for obtaining of specific CoA.