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#18625 Anti-Human Osteopontin (O-17) Rabbit IgG Affinity Purify

  • WB
  • Human kidney
  • 3727_ext_08_en_0_S.jpg
  • 3727_ext_08_en_1_S.jpg
Intended Use:
Research reagents
Application:
WB, IHC
Package Size1:
100 μg
Package Size2:
10 μg
Note on Application Abbreviations
WB:Western Blotting
IHC:Immunohistochemistry

Product Overview

Product Code 18625
Product Name Anti-Human Osteopontin (O-17) Rabbit IgG Affinity Purify
Intended Use Research reagents
Application WB, IHC
Species Human
Immunizing antigen Synthetic peptide of the N-terminal part of human Osteopontin
Purification Method Purified with antigen peptide
Specificity Reacts to both recombinant and native form human Osteopontin.
Can also detect thrombin digested form of human Osteopontin.
Not cross-react with mouse Osteopontin.
Package Form Lyophilized product in PBS containing 1 % BSA and 0.05 % NaN3
Storage Condition 2 - 8℃
Poisonous and Deleterious Substances Applicable
Cartagena Not Applicable
Package Size 1 100 μg
Package Size 2 10 μg
Remarks1 The commercial use of products without our permission is prohibited. Please make sure to contact us and obtain permission.

Product Description

Osteopontin (OPN) is a secreted glycoprotein that was originally isolated from bone. Its molecular weights have been reported in the range of 66 kDa to 44 kDa depending on glycosylation and phosphorylation. OPN is also known to be expressed in other fluids and tissues including milk, urine, activated T cells, smooth muscle cells, kidney and some tumor cells. OPN contains an Arg-Gly-Asp (RGD) amino acid sequence. This motif is present in fibronectin, vitronectin and a variety of other extra-cellular proteins that bind members of the integrin family of cell surface receptors such as αvβ3. OPN was also identified as a ligand for CD44, which levels correlate with aggressiveness of lymphoid tumors and invasiveness of bladder carcinoma. Its interaction does not require RGD motif of OPN. In OPN knockout mice, it has been reported that a significantly decreased level of debridement was shown. Although the distribution and expression pattern of OPN in the human body have suggested the multiple function of OPN, its function under different situations remain obscure. And it is reported the N-terminal OPN fragment cleaved by thrombin (OPN N-Half) binds to α4 or α9 integrin, and the relation with many inflammatory disease is suggested.

FAQ

  • Question
    Q.What is the sequence of the antigen or where is the region?
  • Answer
    A.The sequence is IPVKQADSGSSEEKQ. The region is N-terminal of full length of Osteopontin.
  • Question
    Q.Does this antibody detect full length Osteopontin or Osteopontin cleaved by Thrombin? If the antibody detects Osteopontin cleaved by Thrombin, which part of cleaved Osteopontin is detected by this antibody?
  • Answer
    A.As the region of the antigen is N-terminal of full length Osteopontin, the antibody detects both full length Osteopontion and N-terminal Osteopontin cleaved by Thrombin. This antibody does not detect to C-terminal Osteopontin cleaved by Thrombin.

Note: This product is for research use only. It cannot be used as a diagnostic tool or drug.