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#27363 Rat DMP1 Assay Kit - IBL

Intended Use:
Research reagents
Measuring Method:
ELISA
Sample Types:
Rat
Measuring Samples:
EDTA-plasma, Serum, Cell culture supernatant
Measurement Range:
39.06 ~ 2,500 pg/mL
Package Size1:
96 Well

※ The product indicated as "Research reagents" in the column Intended Use cannot be used
  for diagnostic nor any medical purpose.
※ The datasheet listed on this page is sample only. Please refer to the datasheet
  enclosed in the product purchased before use.

Product Overview

Product Overview

Product Code 27363
Product Name Rat DMP1 Assay Kit - IBL
Intended Use Research reagents
Measuring Method ELISA
Conjugate HRP
Species Rat
Measuring Samples EDTA-plasma, Serum, Cell culture supernatant
Measurement Range 39.06 ~ 2,500 pg/mL
Primary Reaction 60min at 37 ℃
Secondary Reaction 30 minutes at 2 - 8 ℃
Sensitivity 4.04 pg/mL
Specificity Substance Cross Reactivity
Rat DMP1 100.0%
Human DMP1 N.D.
Mouse DMP1 N.D.
Storage Condition 2 - 8 ℃
Poisonous and Deleterious Substances Not Applicable
Cartagena Not Applicable
Measuring Service Not Available
Package Size 1 96 Well

Product Description

Product Description

Dentin Matrix Protein 1(DMP1) is a constitutive protein in extracellular matrix of osteocyte and it belongs to Small Integrin-Binding Ligand, N-linked Glycoprotein (SIBLING) family. It is synthesized as a full-length 105kDa proprotein and it is divided into 37kDa at N terminal side and 57kDa at C terminal side in the secretion process. It has a lot of acidic domains and it binds with calcium because of the feature that become negative charged within tissues and it has an important role for bone mineralization. DMP1 expresses in osteocyte while other constitutive proteins in extracellular matrix such as Osteopontin, Osteocalcin and Bone sialoprotein express in osteoblast cells. Osteocyte which is a main constituent cell of bone regulates activities of osteoclastic cells and osteoblast cells and it adjusts bone remodeling process. It also acts as an endocrine cell which secrets FGF-23. DMP1 in blood is considered as a candidate of biomarker which reflects activities of osteocyte and it is also suggested to become useful information for research of bone metabolism and phosphate metabolism. The concentration of DMP1 in rat blood can be quantitatively measured by this ELISA kit.

References

References

FAQ

FAQ

  • Question
    Q.Can this kit be used for mouse samples?
  • Answer
    A.No, it cannot.
  • Question
    Q.Is composition of EIA buffer of each ELISA kit all same? Can it be mixed to use?
    ELISA common FAQ
  • Answer
    A.No it isn't. As constitute of each EIA buffer is different, it cannot be mixed with other lots or EIA buffers contained in other kind of ELISA kits.
  • Question
    Q.What is the composition of concentrated wash buffer?
    ELISA common FAQ
  • Answer
    A.It contains ordinary Tween and phosphate buffer (0.05% Tween-20 in PB).
  • Question
    Q.What is the feature of the plate?
    ELISA common FAQ
  • Answer
    A.We use plate that is flat bottom and removable strip type plate (8wellx 12 strips).
  • Question
    Q.Can I re-use standard after reconstitution?
    ELISA common FAQ
  • Answer
    A.Not recommended to re-use standard after reconstitution. Please use it at once after the reconstitution.
    Please note that there are some exceptions. One time freeze-thaw the standard is acceptable for use after reconstitution for some ELISAs.
    Please check the details on each product datasheet.
  • Question
    Q.What is different between reagent blank and test sample blank?
    ELISA common FAQ
  • Answer
    A.Reagent blank means a well is only added EIA buffer and the purpose is confirming whether the measuring value is influenced by lack of washing process. Test sample blank means a well is added EIA buffer and HRP antibody and the purpose is to calculate the background.
  • Question
    Q.How many samples can be measured by this kit?
    ELISA common FAQ
  • Answer
    A.The pre-coated plate contained in our ELISA kit is 96 wells plate. We recommend to use 16 wells (2 slits) for standard and 80 wells (10 slits) for 40 samples in duplicate.
  • Question
    Q.What is LOD (Limit of Detection)?
    ELISA common FAQ
  • Answer
    A.It (LOD) is defined as sensitivity that is calculated using the NCCSL method. Please refer to a datasheet of each product.
  • Question
    Q.What is LOQ (Limit of Quantification)?
    ELISA common FAQ
  • Answer
    A.It (LOQ) is the lowest value of measurement (standard) range. Please refer to a datasheet of each product.
  • Question
    Q.What is the definition of Over Night (O/N) reaction?
    ELISA common FAQ
  • Answer
    A.It means that the reaction is required more than 16 hours unless otherwise specifically defined it on a datasheet of each ELISA product.
  • Question
    Q.What is the specification of quality control for ELISA product release?
    ELISA common FAQ
  • Answer
    A.The information of specification is available on individual lot specific CoA. Please contact us with your reference lot number for obtaining of specific CoA.

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