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#27188 Human Syndecan-4 Assay Kit - IBL

  • 27188 Human Syndecan-4 ELISA Kit
  • 27188 Human Syndecan-4 ELISA Kit
  • 27188 Human Syndecan-4 ELISA Kit
  • 27188 Human Syndecan-4 ELISA Kit
  • 27188 Human Syndecan-4 ELISA Kit
  • 27188 Human Syndecan-4 ELISA Kit
Intended Use:
Research reagents
Measuring Method:
ELISA
Sample Types:
Human
Measuring Samples:
EDTA-plasma, Serum, Cell culture supernatant
Measurement Range:
20 ~ 1,280 pg/mL
Package Size1:
96 Well

※ The product indicated as "Research reagents" in the column Intended Use cannot be used
  for diagnostic nor any medical purpose.
※ The datasheet listed on this page is sample only. Please refer to the datasheet
  enclosed in the product purchased before use.

Product Overview

Product Overview

Product Code 27188
Product Name Human Syndecan-4 Assay Kit - IBL
Intended Use Research reagents
Measuring Method ELISA
Conjugate HRP
Species Human
Measuring Samples EDTA-plasma, Serum, Cell culture supernatant
Measurement Range 20 ~ 1,280 pg/mL
Primary Reaction 60 minutes at 37 ℃
Secondary Reaction 30 minutes at 2 - 8 ℃
Sensitivity 3.94 pg/mL
Specificity Compound Cross Reactivity
Human Syndecan-4 100.0%
Human Tenascin-C < 0.1 %
Human Osteopontin < 0.1 %
Human IgG < 0.1 %
Storage Condition 2 - 8 ℃
Poisonous and Deleterious Substances Not Applicable
Cartagena Not Applicable
Measuring Service Not Available
Package Size 1 96 Well

Product Description

Product Description

The syndecan family contains four members (syndecan-1/syndecan, syndecan-2/fibroglycan, syndecan-3/N-syndecan, syndecan-4/ryudocan (amphyglycan)), which are transmembrane heparan sulfate proteoglycans (HSPGs). These HSPGs exhibit cell type-specific distribution with vascular endothelial cells expressing syndecan-1, -2, and -4, and predominant targeting to basolateral surfaces. The syndecan family members are type I integral membrane proteins with homologous transmembrane and cytoplasmic domains. The combined transmembrane/cytoplasmic domains contain four well-conserved tyrosine residues, which might serve important roles for biological function. The cytoplasmic tail of syndecan-4 interacts with focal adhesion molecules. Thus, syndecan-4 is known to have various biological functions such as anticoagulation, cell adhesion and cell multiplication.

The control set is available for sale on request. Learn More

 

FAQ

FAQ

  • Question
    Q.Can this kit be used for mouse or rat samples?
  • Answer
    A.No, it cannot.
  • Question
    Q.What is the epitope of antibodies used in this kit?
  • Answer
    A.Anti-Human Syndecan-4 Rabbit IgG (Precoated Antibody) recognizes position no. 45-57 amino acid of human Syndecan-4.
    Anti-Human Syndecan-4 Rabbit IgG (HRP Antibody) recognizes position no. 19-145 amino acid of human Syndecan-4.
  • Question
    Q.What is the immunogen of 2 antibodies used in the kit?
  • Answer
    A.Synthesized Peptide was used as the immunogen for Anti-Human Syndecan-4 Rabbit IgG (Precoated Antibody).
    Recombinant Protein was used as the immunogen for Anti-Human Syndecan-4 Rabbit IgG (HRP Antibody).
  • Question
    Q.Is composition of EIA buffer of each ELISA kit all same? Can it be mixed to use?
    ELISA common FAQ
  • Answer
    A.No it isn't. As constitute of each EIA buffer is different, it cannot be mixed with other lots or EIA buffers contained in other kind of ELISA kits.
  • Question
    Q.What is the composition of concentrated wash buffer?
    ELISA common FAQ
  • Answer
    A.It contains ordinary Tween and phosphate buffer (0.05% Tween-20 in PB).
  • Question
    Q.What is the feature of the plate?
    ELISA common FAQ
  • Answer
    A.We use plate that is flat bottom and removable strip type plate (8wellx 12 strips).
  • Question
    Q.Can I re-use standard after reconstitution?
    ELISA common FAQ
  • Answer
    A.Not recommended to re-use standard after reconstitution. Please use it at once after the reconstitution.
    Please note that there are some exceptions. One time freeze-thaw the standard is acceptable for use after reconstitution for some ELISAs.
    Please check the details on each product datasheet.
  • Question
    Q.What is different between reagent blank and test sample blank?
    ELISA common FAQ
  • Answer
    A.Reagent blank means a well is only added EIA buffer and the purpose is confirming whether the measuring value is influenced by lack of washing process. Test sample blank means a well is added EIA buffer and HRP antibody and the purpose is to calculate the background.
  • Question
    Q.How many samples can be measured by this kit?
    ELISA common FAQ
  • Answer
    A.The pre-coated plate contained in our ELISA kit is 96 wells plate. We recommend to use 16 wells (2 slits) for standard and 80 wells (10 slits) for 40 samples in duplicate.
  • Question
    Q.What is LOD (Limit of Detection)?
    ELISA common FAQ
  • Answer
    A.It (LOD) is defined as sensitivity that is calculated using the NCCSL method. Please refer to a datasheet of each product.
  • Question
    Q.What is LOQ (Limit of Quantification)?
    ELISA common FAQ
  • Answer
    A.It (LOQ) is the lowest value of measurement (standard) range. Please refer to a datasheet of each product.
  • Question
    Q.What is the definition of Over Night (O/N) reaction?
    ELISA common FAQ
  • Answer
    A.It means that the reaction is required more than 16 hours unless otherwise specifically defined it on a datasheet of each ELISA product.
  • Question
    Q.What is the specification of quality control for ELISA product release?
    ELISA common FAQ
  • Answer
    A.The information of specification is available on individual lot specific CoA. Please contact us with your reference lot number for obtaining of specific CoA.

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