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#27900 Titin N-Fragment Assay Kit - IBL

  • 27900 Titin N Fragment ELISA Kit
  • 27900 Titin N Fragment ELISA Kit
  • 27900 Titin N Fragment ELISA Kit
  • 27900 Titin N Fragment ELISA Kit
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Intended Use:
Research reagents
Measuring Method:
ELISA
Sample Types:
Human, Rat
Measuring Samples:
Urine
Measurement Range:
46.88 ~ 3000 pmol/L
Package Size1:
96 Well

※ The product indicated as "Research reagents" in the column Intended Use cannot be used
for diagnostic nor any medical purpose.

Product Overview

Product Code 27900
Product Name Titin N-Fragment Assay Kit - IBL
Maker Name Immuno-Biological Laboratories Co., Ltd.
Intended Use Research reagents
Measuring Method ELISA
Conjugate HRP
Species Human, Rat
Measuring Samples Urine
Measurement Range 46.88 ~ 3000 pmol/L
Primary Reaction 60 minutes at 37°C
Secondary Reaction 30 minutes at 37℃
Sensitivity 27.91 pmol/L
Storage Condition 2~8°C
Poisonous and Deleterious Substances Not Applicable
Cartagena Not Applicable
Measuring Service Available
Package Size 1 96 Well
Remarks1 This product is no longer available for sale in USA and Europe due to third party's right.

Product Description

Titin (connectin) is a protein that consists of 34,350 amino-acid and specifically expresses in a cross-striated muscle. The molecular weight of human titin is 3,816 kDa and it has been known that as the largest protein among of existing proteins in a living body. It is one of sarcomere structured protein that is a minimum unit of myofibrillary protein and it has a role as an elastic protein for recovering the length of shortened sarcomere by its contraction.  It has been known that titin is cleaved by protein metabolic enzymes such as calpain and matrix metalloprotease if muscles are damaged. In fact, it has been confirmed that titin is dramatically cleaved in skeletal muscles of muscular dystrophy patients and various titin fragments are confirmed in blood and urine.  It has been reported that the N fragment (26kDa) and the C fragment (12kDa) are especially increased in urine of muscular dystrophy patients and it is also suggested that it can be a candidate as a non-invasiveness biomarker for monitoring status of muscles.
This ELISA kit can measure titin N fragment in human and rat urine.

The control set is available for sale on request. Learn More

 

FAQ

  • Question
    Q.Is composition of EIA buffer of each ELISA kit all same? Can it be mixed to use?
    ELISA common FAQ
  • Answer
    A.No it isn't. As constitute of each EIA buffer is different, it cannot be mixed with other lots or EIA buffers contained in other kind of ELISA kits.
  • Question
    Q.What is the composition of concentrated wash buffer?
    ELISA common FAQ
  • Answer
    A.It contains ordinary Tween and phosphate buffer (0.05% Tween-20 in PB).
  • Question
    Q.What is the feature of the plate?
    ELISA common FAQ
  • Answer
    A.We use plate that is flat bottom and removable strip type plate (8wellx 12 strips).
  • Question
    Q.Can I re-use standard after reconstitution?
    ELISA common FAQ
  • Answer
    A.Not recommended to re-use standard after reconstitution. Please use it at once after the reconstitution.
    Please note that there are some exceptions. One time freeze-thaw the standard is acceptable for use after reconstitution for some ELISAs.
    Please check the details on each product datasheet.
  • Question
    Q.What is different between reagent blank and test sample blank?
    ELISA common FAQ
  • Answer
    A.Reagent blank means a well is only added EIA buffer and the purpose is confirming whether the measuring value is influenced by lack of washing process. Test sample blank means a well is added EIA buffer and HRP antibody and the purpose is to calculate the background.
  • Question
    Q.How many samples can be measured by this kit?
    ELISA common FAQ
  • Answer
    A.The pre-coated plate contained in our ELISA kit is 96 wells plate. We recommend to use 16 wells (2 slits) for standard and 80 wells (10 slits) for 40 samples in duplicate.
  • Question
    Q.What is LOD (Limit of Detection)?
    ELISA common FAQ
  • Answer
    A.It (LOD) is defined as sensitivity that is calculated using the NCCSL method. Please refer to a datasheet of each product.
  • Question
    Q.What is LOQ (Limit of Quantification)?
    ELISA common FAQ
  • Answer
    A.It (LOQ) is the lowest value of measurement (standard) range. Please refer to a datasheet of each product.
  • Question
    Q.Do you have any protocol for preparation of mice and/or rat brain
    tissue extracts?
    (ELISA common FAQ * Specified FAQ for ELISA that is applicable for brain tissue extract.)
  • Answer
    A.CHAPS is recommended for extractin of wild type (W.T.) mice or rat brain tissue.
    Please refer to the following link for the brain tissue extraction process using CHAPS.
    http://www.ibl-japan.co.jp/files/topics/4944_ext_02_0.pdf

    Guanidine solution is recommended for extraction of Transgenic (Tg) mice or rat brain tissue.
    Please refer to the following link and the following two scientific articles for brain tissue extraction process using Guanidine.
    http://www.ibl-japan.co.jp/files/topics/4945_ext_02_0.pdf

    Presenilin-2 mutation causes early amyloid accumulation and memory impairment in a transgenic mouse model of Alzheimer's disease. Toda T et al. J Biomed Biotechnol. 2011;2011:617974.
    https://www.ncbi.nlm.nih.gov/pubmed/?term=21234330

    Methylene blue modulates β-secretase, reverses cerebral amyloidosis, and improves cognition in transgenic mice. Mori T et al. J Biol Chem. 2014 Oct 31;289(44):30303-17.
    https://www.ncbi.nlm.nih.gov/pubmed/?term=25157105

    * The ELISA kit #27712 Aβ42 and #27714 Aβ40 have been upgraded to #27719 Aβ (1-42)(FL) and #27718 Aβ (1-40)(FL).

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Note: This product is for research use only. It cannot be used as a diagnostic tool or drug.