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#27900 Titin N-Fragment Assay Kit - IBL

  • 27900 Titin N Fragment ELISA Kit
  • 27900 Titin N Fragment ELISA Kit
  • 27900 Titin N Fragment ELISA Kit
  • 27900 Titin N Fragment ELISA Kit
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Intended Use:
Research reagents
Measuring Method:
ELISA
Sample Types:
Human, Rat
Measuring Samples:
Urine
Measurement Range:
46.88 ~ 3000 pmol/L
Package Size1:
96 Well

Product Overview

Product Code 27900
Product Name Titin N-Fragment Assay Kit - IBL
Maker Name Immuno-Biological Laboratories Co., Ltd.
Intended Use Research reagents
Measuring Method ELISA
Conjugate HRP
Species Human, Rat
Measuring Samples Urine
Measurement Range 46.88 ~ 3000 pmol/L
Primary Reaction 60 minutes at 37°C
Secondary Reaction 30 minutes at 37℃
Sensitivity 27.91 pmol/L
Storage Condition 2~8°C
Poisonous and Deleterious Substances Not Applicable
Cartagena Not Applicable
Measuring Service Available
Package Size 1 96 Well

Product Description

Titin (connectin) is a protein that consists of 34,350 amino-acid and specifically expresses in a cross-striated muscle. The molecular weight of human titin is 3,816 kDa and it has been known that as the largest protein among of existing proteins in a living body. It is one of sarcomere structured protein that is a minimum unit of myofibrillary protein and it has a role as an elastic protein for recovering the length of shortened sarcomere by its contraction.  It has been known that titin is cleaved by protein metabolic enzymes such as calpain and matrix metalloprotease if muscles are damaged. In fact, it has been confirmed that titin is dramatically cleaved in skeletal muscles of muscular dystrophy patients and various titin fragments are confirmed in blood and urine.  It has been reported that the N fragment (26kDa) and the C fragment (12kDa) are especially increased in urine of muscular dystrophy patients and it is also suggested that it can be a candidate as a non-invasiveness biomarker for monitoring status of muscles.
This ELISA kit can measure titin N fragment in human and rat urine.

The control set is available for sale on request. Learn More

Reference
Establishment of a highly sensitive sandwich ELISA for the N-terminal fragment of titin in urine. Maruyama N et al. Sci Rep. 2016 Dec 19;6:39375

FAQ

  • Question
    Q.Is composition of EIA buffer of each ELISA kit all same? Can it be mixed to use?
  • Answer
    A.No it isn't. As constitute of each EIA buffer is different, it cannot be mixed with other lots or EIA buffers contained in other kind of ELISA kits.
  • Question
    Q.What is the composition of concentrated wash buffer?
  • Answer
    A.It contains ordinary Tween and phosphate buffer (0.05% Tween-20 in PB).
  • Question
    Q.What is the feature of the plate?
  • Answer
    A.We use plate that is flat bottom and removable strip type plate (8wellx 12 strips).
  • Question
    Q.Can I re-use standard after reconstitution?
  • Answer
    A.Not recommended to re-use standard after reconstitution. Please use it at once after the reconstitution.
  • Question
    Q.What is different between reagent blank and test sample blank?
  • Answer
    A.Reagent blank means a well is only added EIA buffer and the purpose is confirming whether the measuring value is influenced by lack of washing process. Test sample blank means a well is added EIA buffer and HRP antibody and the purpose is to calculate the background.
  • Question
    Q.How many samples can be measured by this kit?
  • Answer
    A.The pre-coated plate contained in our ELISA kit is 96 wells plate. We recommend to use 16 wells (2 slits) for standard and 80 wells (10 slits) for 40 samples in duplicate.

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Note: This product is for research use only. It cannot be used as a diagnostic tool or drug.