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#27180 Human Serum HTGL Assay Kit - IBL

  • 27180 Human Serum HTGL ELISA Kit
  • 27180 Human Serum HTGL ELISA Kit
  • 27180 Human Serum HTGL ELISA Kit
  • 27180 Human Serum HTGL ELISA Kit
  • 27180 Human Serum HTGL ELISA Kit
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Intended Use:
Research reagents
Measuring Method:
ELISA
Sample Types:
Human
Measuring Samples:
Serum, EDTA-plasma, Postheparin Plasma
Measurement Range:
0.08 - 5 ng/mL
Package Size1:
96 Well

Product Overview

Product Code 27180
Product Name Human Serum HTGL Assay Kit - IBL
Maker Name Immuno-Biological Laboratories Co., Ltd.
Intended Use Research reagents
Measuring Method ELISA
Conjugate HRP
Species Human
Measuring Samples Serum, EDTA-plasma, Postheparin Plasma
Measurement Range 0.08 - 5 ng/mL
Primary Reaction Overnight at 2 - 8 ℃
Secondary Reaction 30 minutes at 2 - 8 ℃
Sensitivity 0.01 ng/mL
Specificity Substance Cross Reactivity
Human LPL ≦ 0.1 %
Human EL ≦ 0.1 %
Storage Condition 2 - 8 ℃
Poisonous and Deleterious Substances Not Applicable
Cartagena Not Applicable
Measuring Service Available
Package Size 1 96 Well

Product Description

HTGL (hepatic triacylglycerol lipase / hepatic triglyceride lipase) is a secreted glycoprotein and known as lipolytic enzyme and is also called as hepatic lipase (HL). HTGL has an important role in lipoprotein metabolism as a lipolytic enzyme that hydrolyzes triglycerides (TG) and phospholipids in chylomicron remnants, intermediate-density lipoproteins (IDL) and high-density lipoproteins (HDL). It has been reported that hypercholesterolemia or triglyceridemia is observed and β-very-low-density lipoproteins (VLDL), chylomicron remnants, IDL, TG-rich low-density lipoproteins (LDL) and HDL are accumulated in patients with deficiency of HTGL. HTGL is synthesized by hepatocytes and bound to heparin sulfate proteoglycans at the surface of liver sinusoidal capillaries. In general speaking, it is essential for use heparin plasma that is collected after intravenous injection of heparin (postheparin plasma) for measurement of HTGL in blood sample as same as lipoprotein lipase (LPL) as HTGL that bonds and adheres to surface of endothelial cells is removed from capillary vascular bed  and released in blood stream soon after the heparin injection. This ELISA kit can detect HTGL in serum and plasma regardless with or without administration of heparin injection that is conventionally required for the measurement.

 

FAQ

  • Question
    Q.Is composition of EIA buffer of each ELISA kit all same? Can it be mixed to use?
  • Answer
    A.No it isn't. As constitute of each EIA buffer is different, it cannot be mixed with other lots or EIA buffers contained in other kind of ELISA kits.
  • Question
    Q.What is the composition of concentrated wash buffer?
  • Answer
    A.It contains ordinary Tween and phosphate buffer (0.05% Tween-20 in PB).
  • Question
    Q.What is the feature of the plate?
  • Answer
    A.We use plate that is flat bottom and removable strip type plate (8wellx 12 strips).
  • Question
    Q.Can I re-use standard after reconstitution?
  • Answer
    A.Not recommended to re-use standard after reconstitution. Please use it at once after the reconstitution.
  • Question
    Q.What is different between reagent blank and test sample blank?
  • Answer
    A.Reagent blank means a well is only added EIA buffer and the purpose is confirming whether the measuring value is influenced by lack of washing process. Test sample blank means a well is added EIA buffer and HRP antibody and the purpose is to calculate the background.
  • Question
    Q.How many samples can be measured by this kit?
  • Answer
    A.The pre-coated plate contained in our ELISA kit is 96 wells plate. We recommend to use 16 wells (2 slits) for standard and 80 wells (10 slits) for 40 samples in duplicate.

Note: This product is for research use only. It cannot be used as a diagnostic tool or drug.