Product related FAQ

A.No, it cannot.

A.This kit can be used for quantitatively measuring total insulin including Insulin I, Insulin II and Proinsulin.

A.Yes it does. We recommend to avoid to use hemolyzed samples for the assay as it might become value due to the effect of insulin-degrading enzymes leaked from blood cells.

A.No it doesn't. There is no much different for the result between with and without Neuramidase.

A.Some samples have a very high value of Gd-IgA1 and such samples would be range over that why the recommended dilution ratio is wide.
We recommend to start from 200 fold dilution and find the best dilution ratio for your samples. The optimized dilution ratio should be determined by each user.

A.Total IgA is approx 1-4mg/mL in blood.

A.As it is approx 1,000 ng/mL in blood, more than 200 fold dilution is recommended for measurement.

A.It is intact IgA1 (IgA Fab+Fc).

A.As certain amount of Gd-IgA1 is also contained in normal subject blood samples and the capture antibody(KM55 monoclonal antibody) used in this kit specifically detects Gd-IgA1 so that human IgA1 is applied as the standard.

A.Recommended dilution ratio is 2-4 fold using “4 EIA buffer” enclosed in this kit.

A.The calibration curve is made by detecting only Gd-IgA1 from the standard.

A.Please refer to the following paper. In the following paper, Gd-IgA1 is generated from human plasma IgA1.
Novel lectin-independent approach to detect galactose-deficient IgA1 in IgA nephropathy.
Yasutake J et al. Nephrol Dial Transplant. 2015 Aug;30(8):1315-21.
https://pubmed.ncbi.nlm.nih.gov/26109484/

A.The HRP conjugated labeled antibody (Anti-Human APP (R101A4) Mouse IgG Fab’ Affinity Purify) used in this kit detects domain at N-Terminal.
The epitope of the precoated plate antibody (Anti-Human sAPPα (2B3) Mouse IgG MoAb Affinity Purify at C-Terminal) used in this kit is DAEFRHDSGYEVHHQK.

A.No, it does not cross react to mouse sAPPα. This kit only detects human sAPPα.

A.It is recombinant protein originated from CHO.

A.Please refer to the section "PERFORMANCE CHARACTERISTICS" on page 2 of the datasheet.
2-4 fold dilution is recommended to start for finding the optimal dilution ratio.
https://www.ibl-japan.co.jp/files/topics/1670_ext_02_en_0.pdf

A.Please refer to the section "PERFORMANCE CHARACTERISTICS" on page 2 of the datasheet.
2-4 fold dilution is recommended to start for finding the optimal dilution ratio.
https://www.ibl-japan.co.jp/files/topics/1670_ext_02_en_0.pdf

A.Please refer to the section "PERFORMANCE CHARACTERISTICS" on page 2 of the datasheet.
2-4 fold dilution is recommended to start for finding the optimal dilution ratio.
https://www.ibl-japan.co.jp/files/topics/1670_ext_02_en_0.pdf

A.Not, it doesn't.

A.The recommended dilution ratio for human serum sample is 2 fold.

A.This kit cross reacts to any type of human soluble APP.

A.No it doesn't.

A.CHAPS is recommended for extractin of wild type (W.T.) mice or rat brain tissue.
Please refer to the following link for the brain tissue extraction process using CHAPS.
http://www.ibl-japan.co.jp/files/topics/4944_ext_02_0.pdf

Guanidine solution is recommended for extraction of Transgenic (Tg) mice or rat brain tissue.
Please refer to the following link and the following two scientific articles for brain tissue extraction process using Guanidine.
http://www.ibl-japan.co.jp/files/topics/4945_ext_02_0.pdf

Presenilin-2 mutation causes early amyloid accumulation and memory impairment in a transgenic mouse model of Alzheimer's disease. Toda T et al. J Biomed Biotechnol. 2011;2011:617974.
https://www.ncbi.nlm.nih.gov/pubmed/?term=21234330

Methylene blue modulates β-secretase, reverses cerebral amyloidosis, and improves cognition in transgenic mice. Mori T et al. J Biol Chem. 2014 Oct 31;289(44):30303-17.
https://www.ncbi.nlm.nih.gov/pubmed/?term=25157105

* The ELISA kit #27712 Aβ42 and #27714 Aβ40 have been upgraded to #27719 Aβ (1-42)(FL) and #27718 Aβ (1-40)(FL).

A.No it doesn't.

A.Yes, it can. However, stability test of the samples should be conducted and confirmed by each institute.

A.Please refer to the datasheet enclosed the product package and/or the datasheet listed on the product page.
As several publications can be found on the page (product description), please refer to them.
https://www.ibl-japan.co.jp/en/search/product/detail/id=3868

A.Yes, it is essential. Please follow the datasheet for the extraction process using column such as SepPak.

A.Yes, it is possible, however, in general speaking, the concentration of Endothelin in blood sample is low conc., the concentration could be much lower after the extraction and process. Please well consider the risk of lack of concentration prior start the measurement.
Our recommendation is more than 2mL blood samples.

A.Only EDTA-plasma collected using collecting tubes contained DPP4 inhibitor can be used for measuring of GLP-1 active form. BD™ P800 GLP-1, GIP, Glucagon, Ghrelin storing vacuumed collecting tube should be used for collecting blood or the blood should be blocked its activity by DPP4 inhibitor prior it is measured. The amount of DPP4 inhibitor for use is 10μL per 1mL blood or 20μL per 1mL plasma.

A.As this kit was developed to measure native GLP-1 active form (7-36/37), we have not evaluated the kit with any commercially available drugs.
Please contact us if you wish to evaluate the kit for the purpose.

A.It is 2 to 8 fold.

A.No, this kit can measure any type of α-Synuclein. It is not specific for phsphorylated form.

A.Wild type mouse or rat samples cannot be used, but APP-transgenic (Tg2576) mouse can be used for the kit.

A.We recommend to use our product #19022 IBLysis.

A.The epitope of HRP conjugated labeled antibody (Anti-Human Aβ (N) (82E1) Mouse IgG MoAb Affinity Purify at N-Terminal) used in this kit is DAEFRHDSGYEVHHQK.
The epitope of the precoated plate antibody (Anti-Human APP-C Rabbit IgG Affinity Purify at C terminal) used in this kit is NGYENPTYKFFEQMQN.

A.No, it cannot.

A.Not recommended to use serum samples. Please use EDTA-plasma samples.

A.We use 2Na.

A.No, it cannot.

A.Not recommended to use serum samples. Please refer to the following article for comparison data between serum and plasma. https://www.ncbi.nlm.nih.gov/pubmed/?term=25045139

A.8 fold dilution is acceptable for the measurement, however, we recommend to dilute mouse urine sample more than 16 fold to avoid the data becomes range over.

A.No, it cannot.

A.It should be stored at -80℃ immediately after collection.

A.It is available on the following relevant product page as images.
http://www.ibl-japan.co.jp/en/search/product/detail/id=4678

A.No, it cannot.

A.The method of collection of mouse urine is listed on the relevant product page.
Please refer to the Pamphlet 2 listed on the page.
http://www.ibl-japan.co.jp/en/search/product/detail/id=4678

A.Klotho has two domains (KL1 and KL2). Although we have not conducted epitope mapping, these antibodies can detect KL1 domains.
The kit was tested using culture supernatants expressing only KL1 of mouse Klotho and has shown a positive response.

A.No it cannot.

A.This assay kit react to both full length FGF 21 (1-181) and FGF21 (7-181). Therefore, this kit can measure total FGF21.

A.5μL is recommended.

A.We have confirmed that it is stable for a week.

A.We have confirmed that it is stable at 37℃ for 2 weeks.

A.5μL is recommended.

A.We have confirmed that it is stable for a week.

A.We have confirmed that it is stable at 37℃ for 2 weeks.

A.2μL is acceptable for measuring GIP Total using samples collected after meal, however at least 5μL is required for measuring it using samples collected in fasting.

A.We have confirmed that it is stable for a week.

A.We have confirmed that it is stable at 37℃ for 2 weeks.

A.2μL is acceptable for measuring GIP Total using samples collected after meal, however at least 5μL is required for measuring it using samples collected in fasting.

A.We have confirmed that it is stable for a week.

A.We have confirmed that it is stable at 37℃ for 2 weeks.

A.No it isn't. As constitute of each EIA buffer is different, it cannot be mixed with other lots or EIA buffers contained in other kind of ELISA kits.

A.It contains ordinary Tween and phosphate buffer (0.05% Tween-20 in PB).

A.We use plate that is flat bottom and removable strip type plate (8wellx 12 strips).

A.Not recommended to re-use standard after reconstitution. Please use it at once after the reconstitution.
Please note that there are some exceptions. One time freeze-thaw the standard is acceptable for use after reconstitution for some ELISAs.
Please check the details on each product datasheet.

A.Reagent blank means a well is only added EIA buffer and the purpose is confirming whether the Test sample value is influenced by lack of washing process or other operations. Test sample blank means a well is added EIA buffer and HRP antibody and the purpose is to calculate the background.

A.The pre-coated plate contained in our ELISA kit is 96 wells plate. We recommend to use 16 wells (2 slits) for standard and 80 wells (10 slits) for 40 samples in duplicate.

A.It (LOD) is defined as sensitivity that is calculated using the NCCSL method. Please refer to a datasheet of each product.

A.It (LOQ) is the lowest value of measurement (standard) range. Please refer to a datasheet of each product.

A.It means that the reaction is required more than 16 hours unless otherwise specifically defined it on a datasheet of each ELISA product.

A.The information of specification is available on individual lot specific CoA. Please contact us with your reference lot number for obtaining of specific CoA.

A.According to the plate maker (ThermoFisher), it does not have any specific meaning as it is just the number of molds.

A.Washing it by an auto-washer is highly recommended.
If it is not available, please refer to the demo video (only 2 mins) using a washing bottle.

A.It is possible that the wells were not washed sufficiently during the washing process after the HRP-labeled antibody reaction.
Be sure to wash the wells enough times as described in the data sheet with washing buffer of more than 350 µL.

A.Only EDTA-plasma collected using collecting tubes contained DPP4 inhibitor can be used for measuring of GLP-1 active form. BD™ P800 GLP-1, GIP, Glucagon, Ghrelin storing vacuumed collecting tube should be used for collecting blood or the blood should be blocked its activity by DPP4 inhibitor prior it is measured. The amount of DPP4 inhibitor for use is 10μL per 1mL blood or 20μL per 1mL plasma.

A.Please start to dilute from 2 fold.

A.It is 2 to 4 fold.

A.Liraglutide can be measured by this kit, however any other drugs are unknown.