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#27704 Rat GIP, Active form (high sensitivity) Assay Kit - IBL

  • 27704 Rat GIP, Active form (high sensitivity) ELISA Kit
  • 27704 Rat GIP, Active form (high sensitivity) ELISA Kit
  • 27704 Rat GIP, Active form (high sensitivity) ELISA Kit
  • 27704 Rat GIP, Active form (high sensitivity) ELISA Kit
Intended Use:
Research reagents
Measuring Method:
ELISA
Sample Types:
Rat
Measuring Samples:
EDTA-plasma
Measurement Range:
0.14 ~ 8.80 pmol/L
Package Size1:
96 Well

※ The product indicated as "Research reagents" in the column Intended Use cannot be used
  for diagnostic nor any medical purpose.
※ The datasheet listed on this page is sample only. Please refer to the datasheet
  enclosed in the product purchased before use.

Product Overview

Product Overview

Product Code 27704
Product Name Rat GIP, Active form (high sensitivity) Assay Kit - IBL
Maker Name Immuno-Biological Laboratories Co., Ltd.
Intended Use Research reagents
Measuring Method ELISA
Conjugate HRP
Species Rat
Measuring Samples EDTA-plasma
Measurement Range 0.14 ~ 8.80 pmol/L
Primary Reaction Overnight at 2 ~ 8°C
Secondary Reaction 60 minutes at 2 ~8°C
Sensitivity 0.06 pmol/L
Specificity Rat GIP(1-42) 100%
Rat GIP(3-42) ≦0.1%
GLP-1(7-36)amide ≦0.1%
GLP-1(9-36)amide ≦0.1%
GLP-1(1-37) ≦0.1%
Glucagon ≦0.1%
Storage Condition 2~8°C
Poisonous and Deleterious Substances Not Applicable
Cartagena Not Applicable
Measuring Service Available
Package Size 1 96 Well
Remarks1 DPP-IV inhibitor has to be added when collecting samples, or use purpose-made blood collection tubes in order to preserve GIP. (eg. BDTM Blood Collection System for Preservation of Plasma GLP-1, GIP, Glucagon and Ghrelin, BD Diagnostics)

Product Description

Product Description

Incretin is a group of gastrointestinal hormones that promotes insulin secretion  from the beta cells of the islets of Langerhans after eating (glucose dependent) and it plays an important role for regulate blood glucose. GIP(glucose-dependent insulinotropic peptide) is incretin as same as GLP-1. It is 42 amino-acid polypeptide and it is secreted from K cells of small intestine by lipid stimulant. Two amino-acid at N-terminal of GIP is rapidly cleaved by DPP-4 in blood and it is inactivated to GIP (3-42). DPP-4 inhibitor that inhibits DPP-4 enzyme which cleaves GIP and GLP-1 and maintain incretin activation has been widely used as useful drug of type 2 diabetes. GIP receptors express not only in beta cell of pancreas but in various organs. It has been reported that especially GIP receptors in adipocyte involve with obesity due to the function of energy deposition. In many cases of the research of glycometabolism, various items are measured at same time and the volume of collected animal samples are limited if the blood needs to be collected in series. This ELISA kit can measure active form rat GIP (1-42) using a tiny amount of rat blood sample.

The control set is available for sale on request. Learn More


Keywords:
GIP / GLP-1 / Insulin / Incretin

 

FAQ

FAQ

  • Question
    Q.How much is the volume of sample required for measurement?
  • Answer
    A.5μL is recommended.
  • Question
    Q.How long does the plate coated biotinylated antibody become stable for use?
  • Answer
    A.We have confirmed that it is stable for a week.
  • Question
    Q.How long does streptavidin plate become stable for use after first opening the pack?
  • Answer
    A.We have confirmed that it is stable at 37℃ for 2 weeks.
  • Question
    Q.Is composition of EIA buffer of each ELISA kit all same? Can it be mixed to use?
    ELISA common FAQ
  • Answer
    A.No it isn't. As constitute of each EIA buffer is different, it cannot be mixed with other lots or EIA buffers contained in other kind of ELISA kits.
  • Question
    Q.What is the composition of concentrated wash buffer?
    ELISA common FAQ
  • Answer
    A.It contains ordinary Tween and phosphate buffer (0.05% Tween-20 in PB).
  • Question
    Q.What is the feature of the plate?
    ELISA common FAQ
  • Answer
    A.We use plate that is flat bottom and removable strip type plate (8wellx 12 strips).
  • Question
    Q.Can I re-use standard after reconstitution?
    ELISA common FAQ
  • Answer
    A.Not recommended to re-use standard after reconstitution. Please use it at once after the reconstitution.
    Please note that there are some exceptions. One time freeze-thaw the standard is acceptable for use after reconstitution for some ELISAs.
    Please check the details on each product datasheet.
  • Question
    Q.What is different between reagent blank and test sample blank?
    ELISA common FAQ
  • Answer
    A.Reagent blank means a well is only added EIA buffer and the purpose is confirming whether the measuring value is influenced by lack of washing process. Test sample blank means a well is added EIA buffer and HRP antibody and the purpose is to calculate the background.
  • Question
    Q.How many samples can be measured by this kit?
    ELISA common FAQ
  • Answer
    A.The pre-coated plate contained in our ELISA kit is 96 wells plate. We recommend to use 16 wells (2 slits) for standard and 80 wells (10 slits) for 40 samples in duplicate.
  • Question
    Q.What is LOD (Limit of Detection)?
    ELISA common FAQ
  • Answer
    A.It (LOD) is defined as sensitivity that is calculated using the NCCSL method. Please refer to a datasheet of each product.
  • Question
    Q.What is LOQ (Limit of Quantification)?
    ELISA common FAQ
  • Answer
    A.It (LOQ) is the lowest value of measurement (standard) range. Please refer to a datasheet of each product.
  • Question
    Q.What is the definition of Over Night (O/N) reaction?
  • Answer
    A.It means that the reaction is required more than 16 hours unless otherwise specifically defined it on a datasheet of each ELISA product.