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#27797 Glucagon ELISA Kit - IBL

  • 27797 Glucagon ELISA Kit
  • 27797 Glucagon ELISA Kit
  • 27797 Glucagon ELISA Kit
Intended Use：
Research reagents
Measuring Method：
ELISA
Sample Types：
Human
Measuring Samples：
EDTA-plasma
Measurement Range：
0.31 - 20 pmol/L
Package Size1：
96 Well

※ The product indicated as "Research reagents" in the column Intended Use cannot be used
　 for diagnostic nor any medical purpose.
※ The datasheet listed on this page is sample only. Please refer to the datasheet
　 enclosed in the product purchased before use.

Product Overview

Product Overview

Product Code 27797
Product Name Glucagon ELISA Kit - IBL
Maker Name Immuno-Biological Laboratories Co., Ltd.
Intended Use Research reagents
Measuring Method ELISA
Conjugate HRP
Species Human
Measuring Samples EDTA-plasma
Measurement Range 0.31 - 20 pmol/L
Primary Reaction 2 - 8°C Overnight
Secondary Reaction 60 minutes at 2 - 8°C
Sensitivity 0.05 pmol/L
Specificity Substance　Cross reactivity(%)
Oxyntomodulin 0.06
Glicentin(1-61) 0.05
Glucagon(3-29) <0.01
Glicentin(1-69) <0.01
Mini-glucagon <0.01
GLP-1(7-36)amide 0.01
GLP-1(9-36)amide 0.01
GLP-2 <0.01
GIP(1-42) <0.01
GIP(3-42) <0.01
Storage Condition 2 - 8°C
Measuring Service Available
Package Size 1 96 Well
Remarks1 ※It is recommended to use purpose made blood collection tubes in order to preserve Glucagon.
(eg. BDTM Blood Collection System for Preservation of Plasma GLP-1, GIP, Glucagon and Ghrelin, BD Biosciences)

Product Description

Product Description

Glucagon is a peptide hormone with a molecular weight of 3,485 consisting of 29 amino acid residues, secreted by the alpha cells of the pancreas. 
It acts on the liver to promote the production and release of glucose through glycogenolysis and glycogenesis, thereby raising blood glucose. 
It has been indicated that dysregulation of glucagon secretion is important in the pathogenesis of diabetes as it is involved with insulin in the regulation of glucose homeostasis.
Proglucagon, a precursor of glucagon produces similar peptides such as glicentin and oxyindomodulin, which have amino acid sequences that overlap with glucagon   by different processing process depending on the produced cell.
It is required to use the assay system which specifically detects glucagon and does not cross reacts to similar peptides for accurately evaluating glucagon in blood.
This ELISA kit can specifically measure glucagon produced in α-cells of pancreas by combining antibodies that recognize both the C-terminus and the N-terminus.　


 

FAQ

FAQ

  • Question
    Q.Is composition of EIA buffer of each ELISA kit all same? Can it be mixed to use?
    ELISA common FAQ
  • Answer
    A.No it isn't. As constitute of each EIA buffer is different, it cannot be mixed with other lots or EIA buffers contained in other kind of ELISA kits.
  • Question
    Q.What is the composition of concentrated wash buffer?
    ELISA common FAQ
  • Answer
    A.It contains ordinary Tween and phosphate buffer (0.05% Tween-20 in PB).
  • Question
    Q.What is the feature of the plate?
    ELISA common FAQ
  • Answer
    A.We use plate that is flat bottom and removable strip type plate (8wellx 12 strips).
  • Question
    Q.Can I re-use standard after reconstitution?
    ELISA common FAQ
  • Answer
    A.Not recommended to re-use standard after reconstitution. Please use it at once after the reconstitution.
    Please note that there are some exceptions. One time freeze-thaw the standard is acceptable for use after reconstitution for some ELISAs.
    Please check the details on each product datasheet.
  • Question
    Q.What is different between reagent blank and test sample blank?
    ELISA common FAQ
  • Answer
    A.Reagent blank means a well is only added EIA buffer and the purpose is confirming whether the Test sample value is influenced by lack of washing process or other operations. Test sample blank means a well is added EIA buffer and HRP antibody and the purpose is to calculate the background.
  • Question
    Q.How many samples can be measured by this kit?
    ELISA common FAQ
  • Answer
    A.The pre-coated plate contained in our ELISA kit is 96 wells plate. We recommend to use 16 wells (2 slits) for standard and 80 wells (10 slits) for 40 samples in duplicate.
  • Question
    Q.What is LOD (Limit of Detection)?
    ELISA common FAQ
  • Answer
    A.It (LOD) is defined as sensitivity that is calculated using the NCCSL method. Please refer to a datasheet of each product.
  • Question
    Q.What is LOQ (Limit of Quantification)?
    ELISA common FAQ
  • Answer
    A.It (LOQ) is the lowest value of measurement (standard) range. Please refer to a datasheet of each product.
  • Question
    Q.What is the definition of Over Night (O/N) reaction?
    ELISA common FAQ
  • Answer
    A.It means that the reaction is required more than 16 hours unless otherwise specifically defined it on a datasheet of each ELISA product.
  • Question
    Q.What is the specification of quality control for ELISA product release?
    ELISA common FAQ
  • Answer
    A.The information of specification is available on individual lot specific CoA. Please contact us with your reference lot number for obtaining of specific CoA.
  • Question
    Q.What is the number (e.g. 432143214321) at the edge of strips of the plate?
    ELISA common FAQ
  • Answer
    A.According to the plate maker (ThermoFisher), it does not have any specific meaning as it is just the number of molds.
  • Question
    Q.How to wash an ELISA plate?
    ELISA common FAQ
  • Answer
    A.Washing it by an auto-washer is highly recommended.
    If it is not available, please refer to the demo video (only 2 mins) using a washing bottle.
  • Question
    Q.The wells turned black during the test with the kit.
    ELISA common FAQ
  • Answer
    A.It is possible that the wells were not washed sufficiently during the washing process after the HRP-labeled antibody reaction.
    Be sure to wash the wells enough times as described in the data sheet with washing buffer of more than 350 µL.
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