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#27900 Human Titin N-Fragment (Urine) ELISA Kit – IBL

  • 27900 Human Titin N-Fragment (Urine) ELISA Kit – IBL
  • 27900 Human Titin N-Fragment (Urine) ELISA Kit – IBL
  • 27900 Human Titin N-Fragment (Urine) ELISA Kit – IBL
  • Correlation data (CPK vs Titin N fragment) #27900
  • 27900 Human Titin N-Fragment (Urine) ELISA Kit – IBL
  • 27900 Human Titin N-Fragment (Urine) ELISA Kit – IBL
  • 27900 Human Titin N-Fragment (Urine) ELISA Kit – IBL
Intended Use：
Research reagents
Measuring Method：
ELISA
Sample Types：
Human
Measuring Samples：
Urine
Measurement Range：
46.88 ～ 3000 pmol/L
Package Size1：
96 Well

※ The product indicated as "Research reagents" in the column Intended Use cannot be used
　 for diagnostic nor any medical purpose.
※ The datasheet listed on this page is sample only. Please refer to the datasheet
　 enclosed in the product purchased before use.

Product Overview

Product Overview

Product Code 27900
Product Name Human Titin N-Fragment (Urine) ELISA Kit – IBL
Maker Name Immuno-Biological Laboratories Co., Ltd.
Intended Use Research reagents
Measuring Method ELISA
Conjugate HRP
Species Human
Measuring Samples Urine
Measurement Range 46.88 ～ 3000 pmol/L
Primary Reaction 60 minutes at 37°C
Secondary Reaction 30 minutes at 37℃
Sensitivity 27.91 pmol/L
Storage Condition 2～8°C
Poisonous and Deleterious Substances Not Applicable
Cartagena Not Applicable
Measuring Service Available
Package Size 1 96 Well

Product Description

Product Description

Titin (connectin) is a protein that consists of 34,350 amino-acid and specifically expresses in a cross-striated muscle. The molecular weight of human titin is 3,816 kDa and it has been known that as the largest protein among of existing proteins in a living body. 

It is one of sarcomere structured protein that is a minimum unit of myofibrillary protein and it has a role as an elastic protein for recovering the length of shortened sarcomere by its contraction.  It has been known that titin is cleaved by protein metabolic enzymes such as calpain and matrix metalloprotease if muscles are damaged. 

It has been researched in the field of sports medicine, cardiac disease, NAFLD, sarcopenia and frailty and it is also considered that it can be a candidate as a non-invasive biomarker for monitoring status of muscles. This ELISA kit can measure Titin N fragment in human urine.

The control set is available for sale on request. Learn More

【Topics】
It was reported that urinary titin can be a non-invasive biomarker for Duchenne muscular dystrophy (DMD).
Learn More　＞External News Link　＞Paper Link

 

References

References

Note: Retrieve by PMID number in displayed by abstract: http://www.ncbi.nlm.nih.gov

FAQ

FAQ

  • Question
    Q.How should we handle urine sample?
  • Answer
    A.1) Please store it frozen immediately after urine collection.
    Please don't leave it at room temperature for more than 8 hours.
    2) It is not necessary to add enzyme inhibitors.
  • Question
    Q.Is any normalization needed for measured value of urine sample?
  • Answer
    A.Yes, it is needed.
    Please measure urinary creatinine at the same time and normalize to it.
  • Question
    Q.Can rodent (mouse or rat) sample be used for this kit?
  • Answer
    A.No, it cannot.
  • Question
    Q.How many time freeze-thaw can be repeated for human urine sample for the assay?
  • Answer
    A.It is stable up to 3 times freeze-thaw repeat. Please refer to the freeze-thaw stability test result from this link.
  • Question
    Q.Do you have any storage (temperature) stability data of urinary titin N-terminal fragment (UTF)?
  • Answer
    A.Yes, we do. Please refer to the data.
  • Question
    Q.Is composition of EIA buffer of each ELISA kit all same? Can it be mixed to use?
    ELISA common FAQ
  • Answer
    A.No it isn't. As constitute of each EIA buffer is different, it cannot be mixed with other lots or EIA buffers contained in other kind of ELISA kits.
  • Question
    Q.What is the composition of concentrated wash buffer?
    ELISA common FAQ
  • Answer
    A.It contains ordinary Tween and phosphate buffer (0.05% Tween-20 in PB).
  • Question
    Q.What is the feature of the plate?
    ELISA common FAQ
  • Answer
    A.We use plate that is flat bottom and removable strip type plate (8wellx 12 strips).
  • Question
    Q.Can I re-use standard after reconstitution?
    ELISA common FAQ
  • Answer
    A.Not recommended to re-use standard after reconstitution. Please use it at once after the reconstitution.
    Please note that there are some exceptions. One time freeze-thaw the standard is acceptable for use after reconstitution for some ELISAs.
    Please check the details on each product datasheet.
  • Question
    Q.What is different between reagent blank and test sample blank?
    ELISA common FAQ
  • Answer
    A.Reagent blank means a well is only added EIA buffer and the purpose is confirming whether the Test sample value is influenced by lack of washing process or other operations. Test sample blank means a well is added EIA buffer and HRP antibody and the purpose is to calculate the background.
  • Question
    Q.How many samples can be measured by this kit?
    ELISA common FAQ
  • Answer
    A.The pre-coated plate contained in our ELISA kit is 96 wells plate. We recommend to use 16 wells (2 slits) for standard and 80 wells (10 slits) for 40 samples in duplicate.
  • Question
    Q.What is LOD (Limit of Detection)?
    ELISA common FAQ
  • Answer
    A.It (LOD) is defined as sensitivity that is calculated using the NCCSL method. Please refer to a datasheet of each product.
  • Question
    Q.What is LOQ (Limit of Quantification)?
    ELISA common FAQ
  • Answer
    A.It (LOQ) is the lowest value of measurement (standard) range. Please refer to a datasheet of each product.
  • Question
    Q.What is the definition of Over Night (O/N) reaction?
    ELISA common FAQ
  • Answer
    A.It means that the reaction is required more than 16 hours unless otherwise specifically defined it on a datasheet of each ELISA product.
  • Question
    Q.What is the specification of quality control for ELISA product release?
    ELISA common FAQ
  • Answer
    A.The information of specification is available on individual lot specific CoA. Please contact us with your reference lot number for obtaining of specific CoA.
  • Question
    Q.What is the number (e.g. 432143214321) at the edge of strips of the plate?
    ELISA common FAQ
  • Answer
    A.According to the plate maker (ThermoFisher), it does not have any specific meaning as it is just the number of molds.
  • Question
    Q.How to wash an ELISA plate?
    ELISA common FAQ
  • Answer
    A.Washing it by an auto-washer is highly recommended.
    If it is not available, please refer to the demo video (only 2 mins) using a washing bottle.
  • Question
    Q.The wells turned black during the test with the kit.
    ELISA common FAQ
  • Answer
    A.It is possible that the wells were not washed sufficiently during the washing process after the HRP-labeled antibody reaction.
    Be sure to wash the wells enough times as described in the data sheet with washing buffer of more than 350 µL.
  • Question
    Q.How do you define the acceptance range of quality control of control sets?
    Control Set Common FAQ
  • Answer
    A.In principal, it is defined as ±15%-20% based the standard value described on each datasheet.
  • Question
    Q.What is the shelf life of control set?
    Control Set Common FAQ
  • Answer
    A.The shelf life is 12 months upon the goods is dispatched from our lab if the goods is unopned.
  • Question
    Q.What is the storage condition of control sets?
    Control Set Common FAQ
  • Answer
    A.It should be stored under chilled condition (2～10℃).
  • Question
    Q.Can the control set be used after reconstitution?
    Control Set Common FAQ
  • Answer
    A.No it cannot be re-used after the reconstitution.
  • Question
    Q.What is the package form of control sets?
    Control Set Common FAQ
  • Answer
    A.It is lyophilized form.
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